Production of soluble αβ T-cell receptor heterodimers suitable for biophysical analysis of ligand binding

Protein Science

Volumn 8, Issue 11, 1999, Pages 2418-2423

  Willcox, Benjamin E ^a   Gao, George F ^a   Wyer, Jessica R ^a   O'Callaghan, Christopher A ^a   Boulter, Jonathan M ^a   Jones, E Yvonne ^a,b   Van Der Merwe, P Anton ^c   Bell, John I ^a   Jakobsen, Bent K ^a  

^a JOHN RADCLIFFE HOSPITAL   (United Kingdom)

^b UNIVERSITY OF OXFORD   (United Kingdom)

^c UNIVERSITY OF OXFORD   (United Kingdom)

Author keywords

Coiled coil; Isothermal titration calorimetry; Protein refolding; Surface plasmon resonance; T cell receptor

Indexed keywords

HYBRID PROTEIN; POLYPEPTIDE; PROTEIN V FOS; STRESS ACTIVATED PROTEIN KINASE; T LYMPHOCYTE RECEPTOR; HLA A2 ANTIGEN; LEUCINE ZIPPER PROTEIN; LIGAND; LYMPHOCYTE ANTIGEN RECEPTOR; PROTEIN C JUN;

ARTICLE; CALORIMETRY; DIMERIZATION; ESCHERICHIA COLI; HLA SYSTEM; INFLUENZA VIRUS A; LIGAND BINDING; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN FOLDING; SURFACE PLASMON RESONANCE; T LYMPHOCYTE; AMINO ACID SEQUENCE; BINDING SITE; BIOPHYSICS; CHEMICAL STRUCTURE; CHEMISTRY; HUMAN; MAJOR HISTOCOMPATIBILITY COMPLEX; METABOLISM; METHODOLOGY; MOLECULAR GENETICS; PROTEIN CONFORMATION; PROTEIN DENATURATION; SOLUBILITY;

ESCHERICHIA COLI; MURINAE; NEGIBACTERIA;

AMINO ACID SEQUENCE; BINDING SITES; BIOPHYSICS; DIMERIZATION; HLA-A2 ANTIGEN; HUMANS; LEUCINE ZIPPERS; LIGANDS; MAJOR HISTOCOMPATIBILITY COMPLEX; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; ONCOGENE PROTEINS V-FOS; PROTEIN CONFORMATION; PROTEIN DENATURATION; PROTEIN FOLDING; PROTO-ONCOGENE PROTEINS C-JUN; RECEPTORS, ANTIGEN, T-CELL, ALPHA-BETA; SOLUBILITY; SURFACE PLASMON RESONANCE;

EID: 0032586023     PISSN: 09618368     EISSN: None     Source Type: Journal    
DOI: 10.1110/ps.8.11.2418     Document Type: Article

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