Hepatic stellate cell-specific gene silencing induced by an artificial MicroRNA for antifibrosis in vitro

Digestive Diseases and Sciences

Volumn 55, Issue 3, 2010, Pages 642-653

  Chang, Ying ^a   Jiang, Hua Jun ^a   Sun, Xue Mei ^a   Cai, Xiao Kun ^a   He, Xing Xing ^a   Li, Pei Yuan ^a   Tang, Wang Xian ^a   Song, Yu Hu ^a   Lin, Ju Sheng ^a  

^a HUAZHONG UNIVERSITY OF SCIENCE AND TECHNOLOGY   (China)

Author keywords

SMA promoter; Liver fibrosis; MicroRNA; RNA interference; TGF 1

Indexed keywords

ALPHA SMOOTH MUSCLE ACTIN; COLLAGEN TYPE 1; ENHANCED GREEN FLUORESCENT PROTEIN; GELATINASE A; MICRORNA; TISSUE INHIBITOR OF METALLOPROTEINASE 1; TRANSFORMING GROWTH FACTOR BETA1; SYNTHETIC DNA;

ANIMAL CELL; ARTICLE; CELL SPECIFICITY; CELL STRAIN HEPG2; CONTROLLED STUDY; DNA MODIFICATION; DRUG MECHANISM; FEASIBILITY STUDY; GENE EXPRESSION REGULATION; GENE EXPRESSION SYSTEM; GENE SILENCING; GENE THERAPY; HUMAN; HUMAN CELL; IN VITRO STUDY; INTRON; LIVER FIBROSIS; MALE; NONHUMAN; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN SYNTHESIS INHIBITION; RAT; REAL TIME POLYMERASE CHAIN REACTION; REPORTER GENE; RNA INTERFERENCE; RNA SEQUENCE; STELLATE CELL; ANIMAL; CELL CULTURE; GENETIC TRANSFECTION; GENETICS; LIVER CIRRHOSIS; POLYMERASE CHAIN REACTION; WISTAR RAT;

ANIMALS; CELLS, CULTURED; COLLAGEN TYPE I; FEASIBILITY STUDIES; GENE SILENCING; GENES, SYNTHETIC; HEPATIC STELLATE CELLS; INTRONS; LIVER CIRRHOSIS; MALE; MATRIX METALLOPROTEINASE 2; MICRORNAS; POLYMERASE CHAIN REACTION; RATS; RATS, WISTAR; TISSUE INHIBITOR OF METALLOPROTEINASE-1; TRANSFECTION; TRANSFORMING GROWTH FACTOR BETA1;

EID: 77949316975     PISSN: 01632116     EISSN: None     Source Type: Journal    
DOI: 10.1007/s10620-009-1021-z     Document Type: Article

References (47)

1. AM Gressner R Weiskirchen 2006 Modern pathogenetic concepts of liver fibrosis suggest stellate cells and TGF-beta as major players and therapeutic targets J Cell Mol Med 10 76 99 10.1111/j.1582-4934.2006.tb00292.x 1:CAS:528:DC%2BD2sXlvF2msL4%3D 16563223 (Pubitemid 44874293)
2. R Bataller DA Brenner 2005 Liver fibrosis J Clin Invest 115 209 218 1:CAS:528:DC%2BD2MXhtFyjs70%3D 15690074 (Pubitemid 40385459)
3. R Bataller YH Paik JN Lindquist JJ Lemasters DA Brenner 2004 Hepatitis C virus core and nonstructural proteins induce fibrogenic effects in hepatic stellate cells Gastroenterology 126 529 540 10.1053/j.gastro.2003.11.018 1:CAS:528:DC%2BD2cXhvV2rtr0%3D 14762790 (Pubitemid 38182310)
4. KR Bridle DH Crawford GA Ramm 2003 Identification and characterization of the hepatic stellate cell transferrin receptor Am J Pathol 162 1661 1667 1:CAS:528:DC%2BD3sXjsl2lsrw%3D 12707050
5. JP Iredale 2007 Models of liver fibrosis: Exploring the dynamic nature of inflammation and repair in a solid organ J Clin Invest 117 539 548 10.1172/JCI30542 1:CAS:528:DC%2BD2sXis12htr4%3D 17332881 (Pubitemid 46348508)
6. Z Qi N Atsuchi A Ooshima A Takeshita H Ueno 1999 Blockade of type beta transforming growth factor signaling prevents liver fibrosis and dysfunction in the rat Proc Natl Acad Sci USA 96 2345 2349 10.1073/pnas.96.5.2345 1:CAS:528:DyaK1MXhvVSqtL0%3D 10051644 (Pubitemid 29117917)
7. EM Zardi A Dobrina G Ambrosino D Margiotta F Polistina A Afeltra 2008 New therapeutic approaches to liver fibrosis: A practicable route? Curr Med Chem 15 1628 1644 10.2174/092986708784911560 1:CAS:528:DC%2BD1cXht1Sgur7I 18673229
8. YH Song XL Chen XJ Kong NZ Liu W Li XL Wu JS Lin YX Jin 2005 Ribozymes against TGFbeta1 reverse character of activated hepatic stellate cells in vitro and inhibit liver fibrosis in rats J Gene Med 7 965 976 10.1002/jgm.744 1:CAS:528:DC%2BD2MXos1aqs7k%3D 15772939 (Pubitemid 41015545)
9. Y Xu B Pasche 2007 TGF-beta signaling alterations and susceptibility to colorectal cancer Hum Mol Genet 16 1 R14 20 10.1093/hmg/ddl486 1:CAS:528:DC%2BD2sXosFOhtLY%3D 17613544 (Pubitemid 47241839)
10. D Schaffert E Wagner 2008 Gene therapy progress and prospects: Synthetic polymer-based systems Gene Ther 15 1131 1138 10.1038/gt.2008.105 1:CAS:528:DC%2BD1cXptlyhsLY%3D 18528432
11. M Zhang EP Smith H Kuroda W Banach SD Chernausek JA Fagin 2002 Targeted expression of a protease-resistant IGFBP-4 mutant in smooth muscle of transgenic mice results in IGFBP-4 stabilization and smooth muscle hypotrophy J Biol Chem 277 21285 21290 10.1074/jbc.M112082200 1:CAS:528:DC%2BD38Xksl2hs7s%3D 11923290 (Pubitemid 34952266)
12. J George D Roulot VE Koteliansky DM Bissell 1999 In vivo inhibition of rat stellate cell activation by soluble transforming growth factor beta type II receptor: A potential new therapy for hepatic fibrosis Proc Natl Acad Sci USA 96 12719 12724 10.1073/pnas.96.22.12719 1:CAS:528:DyaK1MXnt1yqsb4%3D 10535989 (Pubitemid 29513569)
13. R Shi VL Chiang 2005 Facile means for quantifying microRNA expression by real-time PCR Biotechniques 39 519 525 10.2144/000112010 1:CAS:528: DC%2BD2MXhtFWgsL7N 16235564
14. Y Zeng EJ Wagner BR Cullen 2002 Both natural and designed micro RNAs can inhibit the expression of cognate mRNAs when expressed in human cells Mol Cell 9 1327 1333 10.1016/S1097-2765(02)00541-5 1:CAS:528:DC%2BD38Xlt1Sktrk%3D 12086629 (Pubitemid 34722311)
15. Y Zeng BR Cullen 2003 Sequence requirements for micro RNA processing and function in human cells RNA 9 112 123 10.1261/rna.2780503 1:CAS:528: DC%2BD3sXhtFahs7Y%3D 12554881 (Pubitemid 36206307)
16. H Zhou XG Xia Z Xu 2005 An RNA polymerase II construct synthesizes short-hairpin RNA with a quantitative indicator and mediates highly efficient RNAi Nucleic Acids Res 33 e62 10.1093/nar/gni061 15805121 (Pubitemid 41748359)
17. AH Buck J Santoyo-Lopez KA Robertson DS Kumar M Reczko P Ghazal 2007 Discrete clusters of virus-encoded micrornas are associated with complementary strands of the genome and the 7.2-kilobase stable intron in murine cytomegalovirus J Virol 81 13761 13770 10.1128/JVI.01290-07 1:CAS:528: DC%2BD2sXhsValsbbM 17928340 (Pubitemid 350247869)
18. M Castoldi S Schmidt V Benes M Noerholm AE Kulozik MW Hentze MU Muckenthaler 2006 A sensitive array for microRNA expression profiling (miChip) based on locked nucleic acids (LNA) RNA 12 913 920 10.1261/rna.2332406 1:CAS:528:DC%2BD28XksVKrs7g%3D 16540696
19. M Rojkind MA Giambrone L Biempica 1979 Collagen types in normal and cirrhotic liver Gastroenterology 76 710 719 1:CAS:528:DyaE1MXhs1Kjsbc%3D 421999 (Pubitemid 9162968)
20. ER Rayburn R Zhang 2008 Antisense, RNAi, and gene silencing strategies for therapy: Mission possible or impossible? Drug Discov Today 13 513 521 10.1016/j.drudis.2008.03.014 1:CAS:528:DC%2BD1cXnt1als7g%3D 18549978
21. D Kim J Rossi 2008 RNAi mechanisms and applications Biotechniques 44 613 616 10.2144/000112792 1:CAS:528:DC%2BD1cXltlWru7Y%3D 18474035 (Pubitemid 351656279)
22. C Huang M Li C Chen Q Yao 2008 Small interfering RNA therapy in cancer: mechanism, potential targets, and clinical applications Expert Opin Ther Targets 12 637 645 10.1517/14728222.12.5.637 1:CAS:528:DC%2BD1cXks1Sht7s%3D 18410245 (Pubitemid 351699481)
23. DC Rockey 1999 Gene therapy for hepatic fibrosis-bringing treatment into the new millennium Hepatology 30 816 818 10.1002/hep.510300335 1:STN:280:DyaK1MzptFyjtg%3D%3D 10462392 (Pubitemid 29409430)
24. B Spankuch K Strebhardt 2005 RNA interference-based gene silencing in mice: The development of a novel therapeutical strategy Curr Pharm Des 11 3405 3419 10.2174/138161205774370834 16250844 (Pubitemid 41300767)
25. XG Xia H Zhou Z Xu 2006 Transgenic RNAi: Accelerating and expanding reverse genetics in mammals Transgenic Res 15 271 275 10.1007/s11248-006-0023-2 1:CAS:528:DC%2BD28XlvVGrt7Y%3D 16779643 (Pubitemid 43901486)
26. X Cai J Zhou Y Chang X Sun P Li J Lin 2008 Targeting gene therapy for hepatocarcinoma cells with the E. coli purine nucleoside phosphorylase suicide gene system directed by a chimeric alpha-fetoprotein promoter Cancer Lett 264 71 82 10.1016/j.canlet.2008.01.027 1:CAS:528:DC%2BD1cXls12isrw%3D 18407409
27. J Wang W Niu Y Nikiforov S Naito S Chernausek D Witte D LeRoith A Strauch JA Fagin 1997 Targeted overexpression of IGF-I evokes distinct patterns of organ remodeling in smooth muscle cell tissue beds of transgenic mice J Clin Invest 100 1425 1439 10.1172/JCI119663 1:CAS:528:DyaK2sXmt1CqsLg%3D 9294108 (Pubitemid 27425767)
28. RK Moreira 2007 Hepatic stellate cells and liver fibrosis Arch Pathol Lab Med 131 1728 1734 1:CAS:528:DC%2BD2sXhsVSisbrI 17979495
29. S Vogel R Piantedosi J Frank A Lalazar DC Rockey SL Friedman WS Blaner 2000 An immortalized rat liver stellate cell line (HSC-T6): A new cell model for the study of retinoid metabolism in vitro J Lipid Res 41 882 893 1:CAS:528:DC%2BD3cXkt1aktL0%3D 10828080 (Pubitemid 30434319)
30. Y Kim V Ratziu SG Choi A Lalazar G Theiss Q Dang SJ Kim SL Friedman 1998 Transcriptional activation of transforming growth factor beta1 and its receptors by the Kruppel-like factor Zf9/core promoter-binding protein and Sp1. Potential mechanisms for autocrine fibrogenesis in response to injury J Biol Chem 273 33750 33758 10.1074/jbc.273.50.33750 1:CAS:528:DyaK1MXivFE%3D 9837963 (Pubitemid 29005766)
31. XM Zhou JS Lin Y Shi DA Tian HJ Huang HJ Zhou YX Jin 2005 Establishment of a screening system for selection of siRNA target sites directed against hepatitis B virus surface gene Acta Biochim Biophys Sin (Shanghai) 37 310 316 10.1111/j.1745-7270.2005.00049.x 1:CAS:528:DC%2BD2MXlsFSjsL0%3D (Pubitemid 41169478)
32. P Cramer KJ Armache S Baumli S Benkert F Brueckner C Buchen GE Damsma S Dengl SR Geiger AJ Jasiak A Jawhari S Jennebach T Kamenski H Kettenberger CD Kuhn E Lehmann K Leike JF Sydow A Vannini 2008 Structure of eukaryotic RNA polymerases Annu Rev Biophys 37 337 352 10.1146/annurev.biophys.37.032807.130008 1:CAS:528:DC%2BD1cXnsVGluro%3D 18573085
33. Y Lee M Kim J Han KH Yeom S Lee SH Baek VN Kim 2004 MicroRNA genes are transcribed by RNA polymerase II EMBO J 23 4051 4060 10.1038/sj.emboj.7600385 1:CAS:528:DC%2BD2cXotlCrsrs%3D 15372072 (Pubitemid 39472426)
34. SL Lin JD Miller SY Ying 2006 Intronic microRNA (miRNA) J Biomed Biotechnol 2006 26818 17057362
35. N Rajewsky 2006 microRNA target predictions in animals Nat Genet 38 Suppl S8 13 10.1038/ng1798 1:CAS:528:DC%2BD28XltVOmtL4%3D 16736023 (Pubitemid 43920937)
36. A Krek D Grun MN Poy R Wolf L Rosenberg EJ Epstein P MacMenamin I da Piedade KC Gunsalus M Stoffel N Rajewsky 2005 Combinatorial microRNA target predictions Nat Genet 37 495 500 10.1038/ng1536 1:CAS:528:DC%2BD2MXjsF2ksrw%3D 15806104 (Pubitemid 40617277)
37. BP Lewis IH Shih MW Jones-Rhoades DP Bartel CB Burge 2003 Prediction of mammalian microRNA targets Cell 115 787 798 10.1016/S0092-8674(03)01018-3 1:CAS:528:DC%2BD2cXhsFCnsw%3D%3D 14697198 (Pubitemid 38058492)
38. B Zhang Q Wang X Pan 2007 MicroRNAs and their regulatory roles in animals and plants J Cell Physiol 210 279 289 10.1002/jcp.20869 1:CAS:528: DC%2BD28XhtlGqsbjF 17096367 (Pubitemid 44956872)
39. MW Jones-Rhoades DP Bartel B Bartel 2006 MicroRNAS and their regulatory roles in plants Annu Rev Plant Biol 57 19 53 10.1146/annurev.arplant.57.032905. 105218 1:CAS:528:DC%2BD28XosVKhsb0%3D 16669754 (Pubitemid 44061017)
40. MW Rhoades BJ Reinhart LP Lim CB Burge B Bartel DP Bartel 2002 Prediction of plant microRNA targets Cell 110 513 520 10.1016/S0092-8674(02)00863-2 1:CAS:528:DC%2BD38Xmslyjt7s%3D 12202040 (Pubitemid 35232294)
41. TL Cuellar MT McManus 2005 MicroRNAs and endocrine biology J Endocrinol 187 327 332 10.1677/joe.1.06426 1:CAS:528:DC%2BD28XitlOrsQ%3D%3D 16423811 (Pubitemid 43020253)
42. DP Bartel 2004 MicroRNAs: Genomics, biogenesis, mechanism, and function Cell 116 281 297 10.1016/S0092-8674(04)00045-5 1:CAS:528:DC%2BD2cXhtVals7o%3D 14744438 (Pubitemid 38167318)
43. K Cheng N Yang RI Mahato 2009 TGF-beta1 gene silencing for treating liver fibrosis Mol Pharm 6 772 779 10.1021/mp9000469 1:CAS:528:DC%2BD1MXls1Gkurc%3D 19388665
44. KH Kim HC Kim MY Hwang HK Oh TS Lee YC Chang HJ Song NH Won KK Park 2006 The antifibrotic effect of TGF-beta1 siRNAs in murine model of liver cirrhosis Biochem Biophys Res Commun 343 1072 1078 10.1016/j.bbrc.2006.03.087 1:CAS:528:DC%2BD28XksFaiurc%3D 16579972
45. G Li Q Xie Y Shi D Li M Zhang S Jiang H Zhou H Lu Y Jin 2006 Inhibition of connective tissue growth factor by siRNA prevents liver fibrosis in rats J Gene Med 8 889 900 10.1002/jgm.894 1:CAS:528:DC%2BD28XotlSqtb8%3D 16652398 (Pubitemid 44097143)
46. W Xu LW Wang JZ Shi ZJ Gong 2009 Effects of RNA interference targeting transforming growth factor-beta 1 on immune hepatic fibrosis induced by Concanavalin A in mice Hepatobiliary Pancreat Dis Int 8 300 308 1:CAS:528:DC%2BD1MXotlSjtbg%3D 19502172
47. B Schnabl YO Kweon JP Frederick XF Wang RA Rippe DA Brenner 2001 The role of Smad3 in mediating mouse hepatic stellate cell activation Hepatology 34 89 100 10.1053/jhep.2001.25349 1:CAS:528:DC%2BD3MXltlKjs7g%3D 11431738 (Pubitemid 32591487)