The influence of putrefaction and sample storage on post-mortem toxicology results

Forensic Science, Medicine, and Pathology

Volumn 6, Issue 1, 2010, Pages 35-45

  Butzbach, Danielle M ^a  

^a FLINDERS UNIVERSITY   (Australia)

Author keywords

Bacteria; Degradation; Drug stability; Putrefaction; Toxicology

Indexed keywords

ADENOSINE DIPHOSPHATE; ADENOSINE TRIPHOSPHATE; AMPHETAMINE DERIVATIVE; COCAINE; MORPHINE;

BACTERIUM; CADAVER; CLASSIFICATION; FORENSIC TOXICOLOGY; HUMAN; ISOLATION AND PURIFICATION; METABOLISM; METHODOLOGY; OXIDATIVE PHOSPHORYLATION; REVIEW; TIME; URINE;

ADENOSINE DIPHOSPHATE; ADENOSINE TRIPHOSPHATE; AMPHETAMINES; BACTERIA; COCAINE; FORENSIC TOXICOLOGY; HUMANS; MORPHINE; OXIDATIVE PHOSPHORYLATION; POSTMORTEM CHANGES; TIME FACTORS;

EID: 77949284107     PISSN: 1547769X     EISSN: None     Source Type: Journal    
DOI: 10.1007/s12024-009-9130-8     Document Type: Review

References (87)

1. Madea B. Is there recent progess in the estimation of the postmortem interval by means of thanatochemistry? Forensic Sci Int. 2005; 151: 139-49.
2. Vass AA, Barshick S-A, Sega G, Caton J, Skeen JT, JC Love, et al. Decomposition chemistry of human remains: a new methodology for determining the postmortem interval. J Forensic Sci. 2002; 47(3): 542-53.
3. Evans WED. The chemistry of death. Springfield: Charles C Thomas; 1963.
4. Yarema MC, Becker CE. Key concepts in postmortem drug redistribution. Clin Toxicol. 2005; 43: 235-41.
5. Robertson MD, Drummer OH. Postmortem drug metabolism by bacteria. J Forensic Sci. 1995; 40(3): 382-6.
6. Pounder DJ, Jones GR. Post-mortem drug redistribution-a toxicological nightmare. Forensic Sci Int. 1990; 45: 253-63.
7. Paterson S. Drugs and decomposition. Med Sci Law. 1993; 33(2): 103-9.
8. Haglund WD, Sorg MH. Forensic taphonomy: the postmortem fate of human remains. Boca Raton: CRC Press LLC; 1997.
9. Spitz WU. Spitz and Fisher's medicolegal investigation of death. 3rd ed. Springfield: Charles C Thomas; 1993.
10. Bugg T. An introduction to enzyme and coenzyme chemistry. Oxford: Blackwell Science Ltd; 1997.
11. Yamazaki M, Wakasugi C. Postmortem changes in drug-metabolizing enzymes of rat liver microsome. Forensic Sci Int. 1994; 67: 155-68.
12. Micozzi MS. Postmortem change in human and animal remains: a systematic approach. Springfield: Charles C Thomas; 1991.
13. Morris JA, Harrison LM, Partridge SM. Practical and theoretical aspects of postmortem bacteriology. Curr Diagn Pathol. 2007; 13: 65-74.
14. Knight B, Saukko P. Knight's forensic pathology. 3rd ed. London: Arnold Publishers; 2004.
15. Morris JA, Harrison LM, Partridge SM. Postmortem bacteriology: a re-evaluation. J Clin Pathol. 2006; 59: 1-9.
16. Tsokos M, Puschel K. Postmortem bacteriology in forensic pathology: diagnostic value and interpretation. Legal Med. 2001; 3: 15-22.
17. Dix J, Calaluce R, Ernst MF. Guide to forensic pathology. Boca Raton: CRC Press; 1998.
18. Skopp G. Preanalytical aspects in postmortem toxicology. Forensic Sci Int. 2004; 142: 75-100.
19. Dick GL, Stone HM. Alcohol loss arising from microbial contamination of drivers' blood specimens. Forensic Sci Int. 1987; 34: 17-27.
20. Batziris HP, McIntyre IM, Drummer OH. The effect of sulfur-metabolising bacteria on sulfur-containing psychotropic drugs. Int Biodeterior Biodegradation. 1999; 44: 111-6.
21. Rose GW, Hockett RN. The microbiologic evaluation and enumeration of postmortem specimens from human remains. Health Lab Sci. 1971; 8(2): 75-8.
22. Robertson MD, Drummer OH. Stability of nitrobenzodiazepines in postmortem blood. J Forensic Sci. 1998; 43(1): 5-8.
23. Robertson MD, Drummer OH. Postmortem distribution and redistribution of nitrobenzodiazepines in man. J Forensic Sci. 1998; 43(1): 9-13.
24. Robertson MD (1996) The effect of the post-mortem interval on the concentrations of nitrobenzodiazepines. Doctor of Philosophy Thesis, Monash University.
25. Anderson WH, Prouty RW. Postmortem redistribution of drugs. In: Baselt RC, editor. Advances in analytical toxicology VII. Chigaco: Year Book Medical Publishers; 1989. p. 70-102.
26. Rodda KE, Drummer OH. The redistribution of selected psychiatric drugs in post-mortem cases. Forensic Sci Int. 2006; 164: 235-9.
27. Drummer OH. Post-mortem toxicology. Forensic Sci Int. 2007; 165: 199-203.
28. Bertol E, Trignano C, Di Milia MG, Di Padua M, Mari F. Cocaine-related deaths: an enigma still under investigation. Forensic Sci Int. 2008; 176(2-3): 121-3.
29. Logan BK, Smirnow D, Gullberg RG. Lack of predictable site-dependent differences and time-dependent changes in postmortem concentrations of cocaine, benzoylecgonine, and cocaethylene in humans. J Anal Toxicol. 1997; 21: 23-31.
30. Prouty RW, Anderson WH. The forensic science implications of site and temporal influences on postmortem blood-drug concentrations. J Forensic Sci. 1990; 35(2): 243-70.
31. Yonemitsu K, Pounder DJ. Postmortem changes in blood tranylcypromine concentration: competing redistribution and degradation effects. Forensic Sci Int. 1993; 59: 177-84.
32. Drummer OH. Postmortem toxicology of drugs of abuse. Forensic Sci Int. 2004; 142(2-3): 101-13.
33. Curry AS, Sunshine I. The liver: Blood ratio in cases of barbiturate poisoning. Toxicol Appl Pharmacol. 1960; 2: 602-6.
34. Miyaishi S, Moriya F. Immunoassays, forensic applications. In: Payne-James J, editor. Encyclopedia of forensic and legal medicine. 1st ed. Oxford: Elsevier Academic Press; 2005. p. 73-9.
35. Lokan RJ (1977) The forensic significance of putrefactive bases. Masters Thesis, University of Strathclyde.
36. Goeringer KE, Raymon L, Christian GD, Logan BK. Postmortem forensic toxicology of selective serotonin reuptake inhibitors: a review of pharmacology and report of 168 cases. J Forensic Sci. 2000; 45(3): 633-48.
37. Saar E, Gerostamoulos D, Drummer OH. Comparison of extraction efficiencies and LC-MS-MS matrix effects using LLE and SPE methods for 19 antipsychotics in human blood. Anal Bioanal Chem. 2009; 393(2): 727-34.
38. Moriya F, Hashimoto Y. Distribution of free and conjugated morphine in body fluids and tissues in a fatal heroin overdose: is conjugated morphine stable in postmortem specimens? J Forensic Sci. 1997; 42(4): 736-40.
39. Sawyer WR, Forney RB. Postmortem disposition of morphine in rats. Forensic Sci Int. 1988; 38(3-4): 259-73.
40. Spiehler V, Brown R. Unconjugated morphine in blood by radioimmunoassay and gas chromatography/mass spectrometry. J Forensic Sci. 1987; 32(4): 906-16.
41. Mather LE. Opioids: a pharmacologist's delight! Clin Exp Pharmacol Physiol. 1995; 22: 833-6.
42. Rop PP, Grimaldi F, Burle J, De Saint Leger MN, Viala A. Determination of 6-monoacetylmorphine and morphine in plasma, whole blood and urine using high-performance liquid chromatography with electrochemical detection. J Chromatogr B. 1994; 661: 245-53.
43. Skopp G, Potsch L, Klingmann A, Mattern R. Stability of morphine, morphine-3-glucuronide, and morphine-6-glucuronide in fresh blood and plasma and postmortem blood samples. J Anal Toxicol. 2001; 25: 2-7.
44. Stevens HM. The stability of some drugs and poisons in putrefying human liver tissues. J Forensic Sci Soc. 1984; 24: 577-89.
45. Jimenez C, de la Torre R, Ventura M, Segura J, Ventura R. Stability studies of amphetamine and ephedrine derivatives in urine. J Chromatogr B. 2006; 843: 84-93.
46. Nagata T, Kimura K, Hara K, Kudo K. Methamphetamine and amphetamine concentrations in postmortem rabbit tissues. Forensic Sci Int. 1990; 48: 39-47.
47. Moriya F, Hashimoto Y. Postmortem stability of cocaine and cocaethylene in blood and tissues of humans and rabbits. J Forensic Sci. 1996; 41(4): 612-6.
48. Johansen SS, Bhatia HM. Quantitative analysis of cocaine and its metabolites in whole blood and urine by high-performance liquid chromatography coupled with tandem mass spectrometry. J Chromatogr B. 2007; 852: 338-44.
49. Bouis P, Taccard G, Boelsterli UA. Determination of cocaine and norcocaine in plasma and cell cultures using high-performance liquid chromatography. J Chromatogr. 1990; 526: 447-59.
50. Skopp G, Klingman A, Potsch L, Mattern R. In vitro stability of cocaine in whole blood and plasma including ecgonine as a target analyte. Ther Drug Monit. 2001; 23: 174-81.
51. Suzuki O, Watanabe K. Drugs and poisons in humans: a handbook of practical analysis. Basel: Birkhauser; 2005.
52. Levine BS, Blanke RV, Valentour JC. Postmortem stability of benzodiazepines in blood and tissues. J Forensic Sci. 1983; 28(1): 102-15.
53. Entwistle N, Owen P, Patterson DA, Jones LV. The occurrence of chlordiazepoxide degradation products in sudden deaths associated with chlordiazepoxide overdosage. J Forensic Sci Soc. 1986; 26: 45-54.
54. Al-Hadidi KA, Oliver JS. Stability of temazepam in blood. J Forensic Sci Soc. 1995; 35(2): 105-8.
55. Skopp G, Potsch L, Konig I, Mattern R. A preliminary study on the stability of benzodiazepines in blood and plasma stored at 4C. Int J Legal Med. 1998; 111: 1-5.
56. Mahjoub AE. Stability of benzodiazepines in whole blood samples stored at varying temperatures. J Pharm Biomed Anal. 2000; 23(6): 1057-63.
57. Pounder DJ, Hartley AK, Watmough PJ. Postmortem redistribution and degradation of dothiepin. Am J Forensic Med Pathol. 1994; 15(3): 231-5.
58. Caddy B, Stead AH. Three cases of poisoning involving the drug phenelzine. J Forensic Sci Soc. 1978; 18: 207-8.
59. Kwon J-W, Armbrust KL. Laboratory persistence and fate of fluoxetine in aquatic environments. Environ Toxicol Chem. 2006; 25(10): 2561-8.
60. Sykutera M, Pufal E, Sliwka K. The influence of temperature and time of storage on the stability of fluoxetine and biological material. Z Zagadnien Nauk Sadowych. 2002; 50: 5-16.
61. Binsumait IA, Hadidi KA, Abu-Al Raghib S. Stability of fluoxetine in stored plasma, aqueous and methanolic solutions determined by HPLC with UV detection. Die Pharm. 2001; 56(4): 311-3.
62. Asafu-Adjaye EB, Faustino PJ, Tawakkul MA, Anderson LW, Yu LX, Kwon H, et al. Validation and application of a stability-indicating HPLC method for the in vitro determination of gastric and intestinal stability of venlafaxine. J Pharm Biomed Anal. 2007; 43: 1854-9.
63. Coutselinis A, Dimopoulos G, Dritsas C. Fatal intoxication with chlorpromazine with special regard to the influence of putrefaction on its toxicological analysis. Forensic Sci. 1974; 4(2): 191-4.
64. Catlow JT, Barton RD, Clemens M, Gillespie TA, Goodwin M, Swanson SP. Analysis of olanzapine in human plasma utilizing reversed-phase high-performance liquid chromatography with electrochemical detection. J Chromatogr B. 1995; 668: 85-90.
65. Kostakis C, Kenneally M, Felgate P, Walker GS. Difficulties encountered in the analysis of olanzapine from spiked psot-mortem blood samples. Melbourne: TIAFT; 2003.
66. van der Zwaal EM, Luijendijk MCM, Adan RAH, la Fleur SE. Olanzapine-induced weight gain: Chronic infusion using osmotic minipumps does not result in stable plasma levels due to degradation of olanzapine in solution. Eur J Pharmacol. 2008; 585: 130-6.
67. Takayasu T, Ohshima T, Tanaka N, Maeda H, Kondo T, Nishigami J, et al. Postmortem degradation of administered ethanol-d6 and production of endogenous ethanol: experimental studies using rats and rabbits. Forensic Sci Int. 1995; 76: 129-40.
68. Hoiseth G, Karinen R, Chrisophersen AS, Olsen L, Normann PT, Morland J. A study of ethyl glucuronide in post-mortem blood as a marker of ante-mortem ingestion of alcohol. Forensic Sci Int. 2007; 165: 41-5.
69. Canfield D, Brink J, Johnson R, Lewis R, Dubowski K. Clarification of ethanol-positive case using urine serotonin metabolite ratio. J Anal Toxicol. 2007; 31: 592-5.
70. Hoiseth G, Karinen R, Johnson L, Normann PT, Christopherson AS, Morland J. Disappearance of ethyl glucuronide during heavy putrefaction. Forensic Sci Int. 2008; 176(2-3): 147-51.
71. Helander A, Olssen I, Dahl H. Postcollection synthesis of ethyl glucuronide by bacteria in urine may cause false identification of alcohol consumption. Clin Chem. 2007; 53(10): 1855-7.
72. Halter CC, Laengin A, Al-Ahmad A, Wurst FM, Weinmann W, Keummerer K. Assessment of the stability of the ethanol metabolite ethyl sulfate in standardized degradation tests. Forensic Sci Int. 2009; 186: 52-5.
73. Elliot S, Burgess V. Investigative implications of the instability and metabolism of mebeverine. J Anal Toxicol. 2006; 30: 91-7.
74. Koves EM, Lawrence K, Mayer JM. Stability of diltiazem in whole blood: forensic implications. J Forensic Sci. 1998; 43(3): 587-97.
75. 2-agonist concentrations. J Forensic Sci. 1999; 44(3): 523-6.
76. Ramagiri S, Kosanam H, Sai Prakash PK. Stability study of propoxur (baygon) in whole blood and urine stored at varying temperature conditions. J Anal Toxicol. 2006; 30: 313-6.
77. Holmgren P, Druid H, Holmgren A, Ahlner J. Stability of drugs in stored postmortem femoral blood and vitreous humor. J Forensic Sci. 2004; 49(4): 6.
78. Moody DE, Monti KM, Spanbauer AC. Long-term stability of abused drugs and antiabuse chemotherapeutical agents stored at -20C. J Anal Toxicol. 1999; 23: 535-40.
79. Eenoo PV, Lootens L, Spaerkeer A, van Thuyne W, Deventer K, Delbeke FT. Results of stability studies with doping agents in urine. J Anal Toxicol. 2007; 31: 543-8.
80. Lokan RJ, James RA, Dymock RB. Apparent post-mortem production of high levels of cyanide in blood. J Forensic Sci Soc. 1987; 27: 253-9.
81. Elliot SP, Lowe P, Symonds A. The possible influence of micro-organisms and putrefaction in the production of GHB in post-mortem biological fluid. Forensic Sci Int. 2004; 139(2-3): 183-90.
82. Baselt RC. Disposition of toxic drugs and chemicals in man. 6th ed. Foster City: Biomedical Publications; 2002.
83. Berankova K, Mutnanska K, Balikova M. Gamma-hydroxybutyric acid stability and formation in blood and urine. Forensic Sci Int. 2006; 161(2-3): 158-62.
84. Hardman JK, Stadtman TC. Metabolism of g-amino acids: fermentation of g-aminobutyric acid by Clostridium aminobutyricum. J Bacteriol. 1960; 79: 544-8.
85. Levine BS, Blanke RV, Valentour JC. Postmortem stability of barbiturates in blood and tissues. J Forensic Sci. 1984; 29(1): 131-8.
86. Boumba VA, Ziavrou KS, Vougiouklakis T. Biochemical pathways generating post-mortem volatile compounds co-detected during forensic ethanol analyses. Forensic Sci Int. 2008; 174: 133-51.
87. Zaitsu K, Miki A, Katagi M, Tsuchihashi H. Long-term stability of various drugs and metabolites in urine, and preventative measures against their decomposition with special attention to filtration sterilization. Forensic Sci Int. 2008; 174(2-3): 189-96.