Use of fluorescence labelling to monitor protein fractionation by ultrafiltration under controlled permeate flux

Journal of Membrane Science

Volumn 155, Issue 2, 1999, Pages 209-230

  Crespo, J P S G ^a   Trotin, M ^b   Hough, D ^c   Howell, J A ^b  

^a UNIVERSIDADE NOVA DE LISBOA   (Portugal)

^b University of Bath   (United Kingdom)

^c University of Bath   (United Kingdom)

Author keywords

Controlled flux filtration; Fluorescence labelling; Protein fractionation; Protein transmission; Ultrafiltration

Indexed keywords

FLUORESCENCE; MECHANICAL PERMEABILITY; MONITORING; PROTEINS; ULTRAFILTRATION;

CONTROLLED FLUX FILTRATIONS; FLUORESCENCE LABELING; PROTEIN FRACTIONATIONS;

BIOLOGICAL MEMBRANES;

BETA LACTOGLOBULIN; BOVINE SERUM ALBUMIN; FLUORESCENT DYE; IMMUNOGLOBULIN; PROTEIN;

ARTICLE; FLUORESCENCE MICROSCOPY; FRACTIONATION; MEMBRANE; PRIORITY JOURNAL; PROTEIN ANALYSIS; ULTRAFILTRATION;

EID: 0344507361     PISSN: 03767388     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0376-7388(98)00309-3     Document Type: Article

References (24)

1. Nakatsuka S., Michaels A.S. Transport and separation of proteins by ultrafiltration through sorptive and non-sorptive membranes. J. Membr. Sci. 69:1992;189.
2. Rodgers V.G.J., Sparks R.E. Reduction of membrane fouling in ultrafiltration of binary protein mixtures. AIChE J. 37:1991;1517.
3. Kelly S.T., Opong W.S., Zydney A.L. The influence of protein aggregates on the fouling of microfiltration membranes during stirred cell filtration. J. Membr. Sci. 80:1993;175.
4. P. Langer, U. Eichhorn, R. Schnabel, Bioran porous glass membranes for solvent purification, Proceedings of the Fifth World Filtration Congress, Nice, France, 1990, p. 134.
5. M. Nystrom, P. Aimar, S. Luque, M. Kulovaara, S. Metsamuuronen, Fractionation of model proteins using their physiochemical properties, Colloids and Surfaces, A: Physicochemical and Engineering Aspects, vol. 138, 1998, p. 185.
6. Saksena S., Zydney A.L. Effect of solution pH and ionic strength on the separation of albumin from immunoglobulins (IgG) by selective filtration. Biotechnol. Bioeng. 43:1994;960.
7. Nakao S., Osada H., Kurata H., Tsuru T., Kimura S. Separation of proteins by charged ultrafiltration membranes. Desalination. 70:1988;191.
8. Robertson B.C., Zydney A.L. Protein adsorption in asymmetric ultrafiltration membranes with highly constricted pores. J. Colloid Interface Sci. 134:1990;563.
9. Nystrom M., Jarvinen P. Modification of polysulfone ultrafiltration membranes with UV irradiation and hydrophilicity increasing agents. J. Membr. Sci. 60:1991;275.
10. Opong W.S., Zydney A.L. Diffusive and convective protein transport through asymmetric membranes. AIChE J. 37:1991;1497.
11. Balakrishnan M., Agarwal G.P. Protein fractionation in a vortex flow filter. II: Separation of simulated mixtures. J. Membr. Sci. 112:1996;75.
12. Wang Y.Y., Howell J.A., Field R.W., Wu D.X. Simulation of cross-flow filtration for baffled tubular channels and pulsatile flow. J. Membr. Sci. 95:1994;243.
13. Mercier M., Fonade C., Lafforgue-Delorme C. How slug flow can enhance the ultrafiltration flux in mineral tubular membranes. J. Membr. Sci. 128:1997;103.
14. Cabassud C., Laborie S., Laine J.M. How slug flow can improve ultrafiltration flux in organic hollow fibres. J. Membr. Sci. 128:1997;93.
15. Ghosh R., Li Q., Cui Z. Fractionation of BSA and Lysozyme using ultrafiltration: effect of gas sparging. AIChE J. 44:1998;61.
16. Howell J.A., Wu D., Field R.W. Transmission of Bovine Albumin under controlled flux ultrafiltration. J. Membr. Sci. 152:1998;117.
17. Titus J.A., Haugland R., Sharrow S.O., Segal D.M. Texas Red, a hydrophilic, red-emitting fluorophore for the use with fluorescein in dual parameter flow microfluorimetric and fluorescence microscopic studies. J. Immunol. Methods. 50:1982;193.
18. Putting FITC on proteins: look before you label, in: Sigma. Com, vol. 3 (no. 1), 1997, p. 4.
19. Wu D., Howell J.A., Field R.W. Critical flux measurement for model colloids. J. Membr. Sci. 152:1998;89-98.
20. Bradford M.M. A rapid and sensitive method for the quantification of microgram quantities of protein utilising the principle of protein-dye binding. Anal. Biochem. 72:1976;249.
21. Aymard P., Durand D., Nicolai T. The effect of temperature and ionic strength on the dimerisation of β-lactoglobulin. Int. J. Biol. Macromolecules. 19:1996;213.
22. Lentsch S., Aimar P., Orozco J.L. Separation Albumin-PEG: transmission of PEG through ultrafiltration membranes. Biotechnol. Bioeng. 41:1993;1039.
23. van Eijndhoven R.H.C.M., Saksena S., Zydney A.L. Protein fractionation using electrostatic interactions in membrane filtration. Biotechnol. Bioeng. 48:1995;406.
24. Balakrishnan M., Agarwal G.P. Protein fractionation in a vortex flow filter. I: Effect of system hydrodynamics and solution environment on single protein transmission. J. Membr. Sci. 112:1996;47.