Coupling effects of distal loops on structural stability and enzymatic activity of Escherichia coli dihydrofolate reductase revealed by deletion mutants

Biochimica et Biophysica Acta - Proteins and Proteomics

Volumn 1804, Issue 4, 2010, Pages 846-855

  Horiuchi, Yuji ^a   Ohmae, Eiji ^a   Tate, Shin ichi ^a   Gekko, Kunihiko ^a  

^a HIROSHIMA UNIVERSITY   (Japan)

Author keywords

Deletion mutant; DHFR; Enzymatic reaction; Protein folding

Indexed keywords

DIHYDROFOLATE REDUCTASE;

ARTICLE; CATALYSIS; CIRCULAR DICHROISM; DELETION MUTANT; ENZYME ACTIVITY; ENZYME STRUCTURE; ESCHERICHIA COLI; GENE AMPLIFICATION; NONHUMAN; PRIORITY JOURNAL; SITE DIRECTED MUTAGENESIS; STEADY STATE;

CATALYTIC DOMAIN; ENZYME STABILITY; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; KINETICS; MODELS, MOLECULAR; MUTANT PROTEINS; PROTEIN CONFORMATION; PROTEIN FOLDING; RECOMBINANT PROTEINS; SEQUENCE DELETION; TETRAHYDROFOLATE DEHYDROGENASE; THERMODYNAMICS;

ESCHERICHIA COLI;

EID: 76849087798     PISSN: 15709639     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bbapap.2009.12.011     Document Type: Article

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