Stretching exercises - Flexibility in dihydrofolate reductase catalysis

Chemistry and Biology

Volumn 5, Issue 5, 1998, Pages

  Miller, Grover Paul ^a   Benkovic, Stephen J ^a  

^a PENNSYLVANIA STATE UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 0031808658     PISSN: 10745521     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1074-5521(98)90616-0     Document Type: Review

References (31)

1. Charlton, P.A., Young, D.W., Birdsall, B., Feeny, J. & Roberts, G.C.K. (1979). Sterochemistry of reduction of folio acid using dihydrofolate reductase. J. Chem. Soc. Chem. Commun. 922-924.
2. Fierke, C.A, Johnson, K.A. & Benkovic, S.J. (1987). Construction and evaluation of the kinetic scheme associated with dihydrofolate reductase from Escherichia coli. Biochemistry 26, 4085-4092.
3. Sawaya, M.R. & Kraut, J. (1997). Loop and subdomain movements in the mechanism of Escherichia coli dihydrofolate reductase: crystallographic evidence. Biochemistry 36, 586-603.
4. 1H assignments of the Escherichia coli dihydrofolate reductase complex with folate: Evidence for a unique conformation of bound folate. Biochemistry 29, 9667-9677.
5. Mesecar, A.D., Stoddard, B.L. & Koshland, D.E. (1997). Orbital steering in the catalytic power of enzymes: small structural changes with large catalytic consequences. Science 277, 202-206.
6. Benkovic, S.J., Fierke, C.A. & Naylor, A.M. (1988). Insights into enzyme function from studies on mutants of dihydrofolate reductase. Science 239, 1105-1110.
7. Wu, Y.D. & Houk, K.N. (1987). Theoretical transition structures for hydride transfer to metheniminium ion from methylamine and dihydropyridine. On the nonlinearity of hydride transfers. J. Am. Chem. Soc. 109, 2226-2227.
8. + ternary complex. Substrate binding and a model for the transition state. Biochemistry 29, 3263-3277.
9. Appleman, J.R., Beard, W.A., Delcamp, T.J., Prendergast, N.J., Freisheim, J.H. & Blakely, R.L. (1990). Role of aspartate 27 of dihydrofolate reductase from Escherichia coli in interconversion of active and inactive enzyme conformers and binding of NADPH. J. Biol. Chem. 265, 2740-2748.
10. Howell, E.E., Villafranca, J.E., Warren, M.S., Oatley, S.J. & Kraut, J. (1986). Functional role of aspartic acid 27 in dihydrofolate reductase revealed by site-directed mutagenesis. Science 231, 1123-1128.
11. Wagner, C.R., Thillet, J. & Benkovic, S.J. (1992). Complementary perturbation of the kinetic mechanism and catalytic effectiveness of dihydrofolate reductase by side-chain interchange. Biochemistry 31, 7834-7840.
12. Huang, Z., Wagner, C.R. & Benkovic, S.J. (1994). Nonadditivity of mutational effects at the folate binding site of Escherichia coli dihydrofolate reductase. Biochemistry 33, 11576-11585.
13. Chen, J.-T., Taira, K., Tu, C.P.D. & Benkovic, S.J. (1987). Probing the functional role of phenylalanine-31 of Escherichia coli dihydrofolate reductase by site-directed mutagenesis. Biochemistry 26, 4093-4100.
14. Murphy, D.J. & Benkovic, S.J. (1989). Hydrophobic interactions via mutants of Escherichia coli dihydrofolate reductase: Separation of binding and catalysis. Biochemistry 28, 3025-3031.
15. Fierke, C.A. & Benkovic, S.J. (1989). Probing the functional role of threonine-113 of Escherichia coli dihydrofolate reductase for its effect on turnover efficiency, catalysis, and binding. Biochemistry 28, 478-486.
16. Adams, J., Johnson, K., Matthews, R. & Benkovic, S.J. (1989). Effects of distal point-site mutations on the binding and catalysis of dihydrofolate reductase from Escherichia coli. Biochemistry 28, 6611-6618.
17. Adams, J.A., Fierke, C.A. & Benkovic, S.J. (1991). The function of the amino acid residues contacting the nicotinamide ring of NADPH in dihydrofolate reductase from Escherichia coli. Biochemistry 30, 11046-11054.
18. Warren, M.S., Brown, K.A., Farnum, M.F., Howell, E.E. & Kraut, J. (1991). Investigation of the functional role of tryptophan 22 in Escherichia coli dihydrofolate reductase by site-directed mutagenesis. Biochemistry 30, 11092-11103.
19. Lee, H., Reyes, V.M. & Kraut, J. (1996). Crystal structures of Escherichia coli dihydrofolate reductase complexed with 5-formyltetrahydrofolate (folinic acid), in two space groups: evidence for enolization of pteridine O4. Biochemistry 35, 7012-7020.
20. Chen, Y.Q., Kraut, J., Blakely, R.L. & Callender, R. (1994). Determination by Raman spectroscopy of the pKa of N5 of dihydrofolate bound to dihydrofolate reductase: mechanistic implications. Biochemistry 33, 7021-7026.
21. Cannon, W.R., Garrison, B.J. & Benkovic, S.J. (1997). Electrostatic characterization of enzyme complexes: evalution of the mechanism of catalysis of dihydrofolate reductase. J. Am. Chem. Soc. 119, 2386-2395.
22. Li, L., Falzone, C.J., Wright, P.E. & Benkovic, S.J. (1992). Functional role of a mobile loop of Escherichia coli dihydrofolate reductase in transition-state stabilization. Biochemistry 31, 7826-7833.
23. Gerstein, M., Lesk, A.M. & Chothia, C. (1994). Structural mechanisms for domain movements in proteins. Biochemistry 33, 6739-6749.
24. Wagner, C.R., Huang, Z., Singleton, S. & Benkovic, S.J.(1995). Molecular basis for nonadditive mutational effects in Escherichia coli dihydrofolate reductase. Biochemistry 34, 15671-15680.
25. Zheng, J., Chen, Y.Q. & Callender, R. (1993). A study of the binding of NADP coenzymes to dihydrofolate reductase by Raman difference spectroscopy. Eur. J. Biochem. 215, 9-16.
26. Bystroff, C. & Kraut, J. (1991). Crystal structures of unliganded Escherichia coli dihydrofolate reductase: Ligand-induced conformational changes and cooperativity in binding. Biochemistry 30, 2227-2239.
27. Falzone, C.J., Wright, P.E. & Benkovic, S.J. (1994). Dynamics of a flexible loop in dihydrofolate reductase from Escherichia coli and its importance for catalysis. Biochemistry 33, 439-442.
28. Epstein, D.M., Benkovic, S.J. & Wright, P.E. (1995). Dynamics of the dihydrofolate reductase-folate compelx: Catalytic sites and regions known to undergo conformational changes exhibit diverse dynamical features. Biochemistry 34, 11037-11048.
29. Cameron, C.E. & Benkovic, S.J. (1997). Evidence for a functional role of the dynamics of glycine 121 of Escherichia coli dihydrofolate reductase obtained from kinetic analysis of a sitedirected mutant. Biochemistry, 36, 15792-15800.
30. Miller, G.P. & Benkovic, S.J. (1998). Deletion of a highly motional residue affects formation of the Michaelis complex for Escherichia coli dihydrofolate reductase. Biochemistry, in press.
31. Miller, G.P. & Benkovic, S.J. (1988). Strength of a hydrogen bond determines the kinetic pathway in catalysis by Escherichia coli dihydrofolate reductase. Biochemistry, in press.