-
1
-
-
0020441466
-
Crystal structures of Escherichia coli and Lactobacillus casei dihydrofolate reductase refined at 1.7 Å resolution
-
Bolin, J.T., Filman, D.J., Matthews, D.A., Hamlin, R.C., and Kraut, J. (1982) Crystal structures of Escherichia coli and Lactobacillus casei dihydrofolate reductase refined at 1.7 Å resolution. J. Biol. Chem. 257, 13650-13662
-
(1982)
J. Biol. Chem.
, vol.257
, pp. 13650-13662
-
-
Bolin, J.T.1
Filman, D.J.2
Matthews, D.A.3
Hamlin, R.C.4
Kraut, J.5
-
2
-
-
0025270551
-
+ ternary complex. Substrate binding and a model for the transition state
-
+ ternary complex. Substrate binding and a model for the transition state. Biochemistry 29, 3263-3277
-
(1990)
Biochemistry
, vol.29
, pp. 3263-3277
-
-
Bystroff, C.1
Oatley, S.J.2
Kraut, J.3
-
3
-
-
0026065644
-
Crystal structure of unliganded Escherichia coli dihydrofolate reductase: Ligand-induced conformational changes and cooperativity in binding
-
Bystroff, C. and Kraut, J. (1991) Crystal structure of unliganded Escherichia coli dihydrofolate reductase: Ligand-induced conformational changes and cooperativity in binding. Biochemistry 30, 2227-2239
-
(1991)
Biochemistry
, vol.30
, pp. 2227-2239
-
-
Bystroff, C.1
Kraut, J.2
-
4
-
-
0029102089
-
Dynamics of the dihydrofolate reductase-folate complex: Catalytic sites and regions known to undergo conformational change exhibit diverse dynamical features
-
Epstein, D.M., Benkovic, S.J., and Wright, P.E. (1995) Dynamics of the dihydrofolate reductase-folate complex: catalytic sites and regions known to undergo conformational change exhibit diverse dynamical features. Biochemistry 34, 11037-11048
-
(1995)
Biochemistry
, vol.34
, pp. 11037-11048
-
-
Epstein, D.M.1
Benkovic, S.J.2
Wright, P.E.3
-
5
-
-
0027411746
-
Effects of point mutations in a flexible loop on the stability and enzymatic function of Escherichia coli dihydrofolate reductase
-
Gekko, K., Yamagami, K., Kunori, Y., Ichihara, S., Kodama, M., and Iwakura, M. (1993) Effects of point mutations in a flexible loop on the stability and enzymatic function of Escherichia coli dihydrofolate reductase. J. Biochem. 113, 74-80
-
(1993)
J. Biochem.
, vol.113
, pp. 74-80
-
-
Gekko, K.1
Yamagami, K.2
Kunori, Y.3
Ichihara, S.4
Kodama, M.5
Iwakura, M.6
-
6
-
-
0027998347
-
Point mutations at glycine-121 of Escherichia coli dihydrofolate reductase: Important roles of a flexible loop in the stability and function
-
Gekko, K., Kunori, Y., Takeuchi, H., Ichihara, S., and Kodama, M. (1994) Point mutations at glycine-121 of Escherichia coli dihydrofolate reductase: Important roles of a flexible loop in the stability and function. J. Biochem. 116, 34-41
-
(1994)
J. Biochem.
, vol.116
, pp. 34-41
-
-
Gekko, K.1
Kunori, Y.2
Takeuchi, H.3
Ichihara, S.4
Kodama, M.5
-
7
-
-
0029939820
-
Effects of point mutations at the flexible loop glycine-67 of Escherichia coli dihydrofolate reductase on its stability and function
-
Ohmae, E., Iriyama, K., Ichihara, S., and Gekko, K. (1996) Effects of point mutations at the flexible loop glycine-67 of Escherichia coli dihydrofolate reductase on its stability and function. J. Biochem. 119, 703-710
-
(1996)
J. Biochem.
, vol.119
, pp. 703-710
-
-
Ohmae, E.1
Iriyama, K.2
Ichihara, S.3
Gekko, K.4
-
8
-
-
0022994226
-
Mutant forms of staphylococcal nuclease with altered patterns of guanidine hydrochloride and urea denaturation
-
Shortle, D. and Meeker, A.K. (1986) Mutant forms of staphylococcal nuclease with altered patterns of guanidine hydrochloride and urea denaturation. Proteins: Struct. Funct. Genet. 1, 81-89
-
(1986)
Proteins: Struct. Funct. Genet.
, vol.1
, pp. 81-89
-
-
Shortle, D.1
Meeker, A.K.2
-
9
-
-
0022965255
-
Stabilization of lambda repressor against thermal denaturation by site-directed Gly→Ala changes in alpha-helix 3
-
Hecht, M.H., Sturtevant, J.M., and Sauer, R.T. (1986) Stabilization of lambda repressor against thermal denaturation by site-directed Gly→Ala changes in alpha-helix 3. Proteins: Struct. Funct. Genet. 1, 43-46
-
(1986)
Proteins: Struct. Funct. Genet.
, vol.1
, pp. 43-46
-
-
Hecht, M.H.1
Sturtevant, J.M.2
Sauer, R.T.3
-
10
-
-
0023039204
-
Synergism in folding of a double mutant of the alpha subunit of tryptophan synthase
-
Hurle, M.R., Tweedy, N.B., and Matthews, C.R. (1986) Synergism in folding of a double mutant of the alpha subunit of tryptophan synthase. Biochemistry 25, 6356-6360
-
(1986)
Biochemistry
, vol.25
, pp. 6356-6360
-
-
Hurle, M.R.1
Tweedy, N.B.2
Matthews, C.R.3
-
11
-
-
0023776704
-
Disulfide bonds and thermal stability in T4 lysozyme
-
Wetzel, R., Perry, L.J., Baase, W.A., and Becktel, W.J. (1988) Disulfide bonds and thermal stability in T4 lysozyme. Proc. Natl. Acad. Sci. USA 85, 401-405
-
(1988)
Proc. Natl. Acad. Sci. USA
, vol.85
, pp. 401-405
-
-
Wetzel, R.1
Perry, L.J.2
Baase, W.A.3
Becktel, W.J.4
-
12
-
-
0027248706
-
Engineering multiple properties of a protein by combinatorial mutagenesis
-
Sandberg, W.S. and Terwilliger, T.C. (1993) Engineering multiple properties of a protein by combinatorial mutagenesis. Proc. Natl. Acad. Sci. USA 90, 8367-8371
-
(1993)
Proc. Natl. Acad. Sci. USA
, vol.90
, pp. 8367-8371
-
-
Sandberg, W.S.1
Terwilliger, T.C.2
-
13
-
-
0029078751
-
High resistance of Escherichia coli ribonuclease HI variant with quintuple thermostabilizing mutations to thermal denaturation, acid denaturation, and proteolytic degradation
-
Akasako, A., Haruki, M., Oobatake, M., and Kanaya, S. (1995) High resistance of Escherichia coli ribonuclease HI variant with quintuple thermostabilizing mutations to thermal denaturation, acid denaturation, and proteolytic degradation. Biochemistry 34, 8115-8122
-
(1995)
Biochemistry
, vol.34
, pp. 8115-8122
-
-
Akasako, A.1
Haruki, M.2
Oobatake, M.3
Kanaya, S.4
-
14
-
-
0031021982
-
Analysis of binding interactions in an idiotope-anti-idiotope protein-protein complex by double mutant cycles
-
Goldman, E.R., Dall'Acqua, W., Braden, B.C., and Mariuzza, R.A. (1997) Analysis of binding interactions in an idiotope-anti-idiotope protein-protein complex by double mutant cycles. Biochemistry 36, 49-56
-
(1997)
Biochemistry
, vol.36
, pp. 49-56
-
-
Goldman, E.R.1
Dall'Acqua, W.2
Braden, B.C.3
Mariuzza, R.A.4
-
15
-
-
0025093185
-
Estimating the contribution of engineered surface electrostatic interactions to protein stability by using double-mutant cycles
-
Serrano, L., Horovitz, A., Avron, B., Bycroft, M., and Fersht, A.R. (1990) Estimating the contribution of engineered surface electrostatic interactions to protein stability by using double-mutant cycles. Biochemistry 29, 9343-9352
-
(1990)
Biochemistry
, vol.29
, pp. 9343-9352
-
-
Serrano, L.1
Horovitz, A.2
Avron, B.3
Bycroft, M.4
Fersht, A.R.5
-
16
-
-
0027383834
-
Patterns of nonadditivity between pairs of stability mutations in staphylococcal nuclease
-
Green, S.M. and Shortle, D. (1993) Patterns of nonadditivity between pairs of stability mutations in staphylococcal nuclease. Biochemistry 32, 10131-10139
-
(1993)
Biochemistry
, vol.32
, pp. 10131-10139
-
-
Green, S.M.1
Shortle, D.2
-
17
-
-
0028920304
-
Alanine scanning mutagenesis of the alpha-helix 115-123 of phage T4 lysozyme: Effects on structure, stability and the binding of solvent
-
Blaber, M., Baase, W.A., Gassner, N., and Matthews, B.W. (1995) Alanine scanning mutagenesis of the alpha-helix 115-123 of phage T4 lysozyme: Effects on structure, stability and the binding of solvent. J. Mol. Biol. 246, 317-330
-
(1995)
J. Mol. Biol.
, vol.246
, pp. 317-330
-
-
Blaber, M.1
Baase, W.A.2
Gassner, N.3
Matthews, B.W.4
-
18
-
-
0024457568
-
Long-range electrostatic interactions can influence the folding, stability, and cooperativity of dihydrofolate reductase
-
Perry, K.M., Onuffer, J.J., Gittelman, M.S., Barmat, L., and Matthews, C.R. (1989) Long-range electrostatic interactions can influence the folding, stability, and cooperativity of dihydrofolate reductase. Biochemistry 28, 7961-7968
-
(1989)
Biochemistry
, vol.28
, pp. 7961-7968
-
-
Perry, K.M.1
Onuffer, J.J.2
Gittelman, M.S.3
Barmat, L.4
Matthews, C.R.5
-
19
-
-
0026648195
-
Intramolecular catalysis of a proline isomerization reaction in the folding of dihydrofolate reductase
-
Texter, F.L., Spencer, D.B., Rosenstein, R., and Matthews, C.R. (1992) Intramolecular catalysis of a proline isomerization reaction in the folding of dihydrofolate reductase. Biochemistry 31, 5687-5691
-
(1992)
Biochemistry
, vol.31
, pp. 5687-5691
-
-
Texter, F.L.1
Spencer, D.B.2
Rosenstein, R.3
Matthews, C.R.4
-
20
-
-
0027174068
-
How do mutations at phenylalanine-153 and isoleucine-155 partially suppress the effects of the aspartate-27→ serine mutation in Escherichia coli dihydrofolate reductase?
-
Dion, A., Linn, C.E., Bradrick, T.D., Georghiou, S., and Howell, E.E. (1993) How do mutations at phenylalanine-153 and isoleucine-155 partially suppress the effects of the aspartate-27→ serine mutation in Escherichia coli dihydrofolate reductase? Biochemistry 32, 3479-3487
-
(1993)
Biochemistry
, vol.32
, pp. 3479-3487
-
-
Dion, A.1
Linn, C.E.2
Bradrick, T.D.3
Georghiou, S.4
Howell, E.E.5
-
21
-
-
0026537527
-
Dihydrofolate reductase gene as a versatile expression marker
-
Iwakura, M. and Tanaka, T. (1992) Dihydrofolate reductase gene as a versatile expression marker. J. Biochem. 111, 31-36
-
(1992)
J. Biochem.
, vol.111
, pp. 31-36
-
-
Iwakura, M.1
Tanaka, T.2
-
22
-
-
0017681196
-
DNA sequencing with chain-terminating inhibitors
-
Sanger, F., Nicklen, S., and Coulson, A.R. (1977) DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA 74, 5463-5467
-
(1977)
Proc. Natl. Acad. Sci. USA
, vol.74
, pp. 5463-5467
-
-
Sanger, F.1
Nicklen, S.2
Coulson, A.R.3
-
23
-
-
0022344741
-
Isolation of a dihydrofolate reductase-deficient mutant of Escherichia coli
-
Singer, S., Ferone, R., Walton, L., and Elwell, L. (1985) Isolation of a dihydrofolate reductase-deficient mutant of Escherichia coli. J. Bacteriol. 164, 470-472
-
(1985)
J. Bacteriol.
, vol.164
, pp. 470-472
-
-
Singer, S.1
Ferone, R.2
Walton, L.3
Elwell, L.4
-
24
-
-
0023668190
-
Construction and evaluation of the kinetic scheme associated with dihydrofolate reductase from Escherichia coli
-
Fierke, C.A., Johnson, K.A., and Benkovic, S.J. (1987) Construction and evaluation of the kinetic scheme associated with dihydrofolate reductase from Escherichia coli. Biochemistry 26, 4085-4092
-
(1987)
Biochemistry
, vol.26
, pp. 4085-4092
-
-
Fierke, C.A.1
Johnson, K.A.2
Benkovic, S.J.3
-
25
-
-
0002465965
-
-
(Matsushita, K., ed.) North Holland Publishing Company, Amsterdam
-
Nakagawa, T. and Oyanagi, Y. (1980) Program system SALS for nonlinear least-squares fitting in experimental sciences in Recent Developments in Statistical Inference and Data Analysis (Matsushita, K., ed.) pp. 221-225, North Holland Publishing Company, Amsterdam
-
(1980)
Program System SALS for Nonlinear Least-squares Fitting in Experimental Sciences in Recent Developments in Statistical Inference and Data Analysis
, pp. 221-225
-
-
Nakagawa, T.1
Oyanagi, Y.2
-
26
-
-
0000562154
-
-
(Hirs, C.H.W. and Timasheff, S.N., eds.) Academic Press, New York
-
Pace, C.N. (1985) Determination and analysis of urea and guanidine hydrochloride denaturation curves in Methods in Enzymology (Hirs, C.H.W. and Timasheff, S.N., eds.) Vol. 131, pp. 267-280, Academic Press, New York
-
(1985)
Determination and Analysis of Urea and Guanidine Hydrochloride Denaturation Curves in Methods in Enzymology
, vol.131
, pp. 267-280
-
-
Pace, C.N.1
-
27
-
-
0018759051
-
Methotrexate, a high-affinity pseudosubstrate of dihydrofolate reductase
-
Williams, J.W., Morrison, J.F., and Duggleby, R.G. (1979) Methotrexate, a high-affinity pseudosubstrate of dihydrofolate reductase. Biochemistry 18, 2567-2573
-
(1979)
Biochemistry
, vol.18
, pp. 2567-2573
-
-
Williams, J.W.1
Morrison, J.F.2
Duggleby, R.G.3
-
28
-
-
0021872251
-
Substrate-induced hysteresis in the activity of Escherichia coli dihydrofolate reductase
-
Penner, M.H. and Frieden, C. (1985) Substrate-induced hysteresis in the activity of Escherichia coli dihydrofolate reductase. J. Biol. Chem. 260, 5366-5369
-
(1985)
J. Biol. Chem.
, vol.260
, pp. 5366-5369
-
-
Penner, M.H.1
Frieden, C.2
-
29
-
-
0020466628
-
Kinetic mechanism of the reaction catalyzed by dihydrofolate reductase from Escherichia coli
-
Stone, S.R. and Morrison, J.F. (1982) Kinetic mechanism of the reaction catalyzed by dihydrofolate reductase from Escherichia coli. Biochemistry 21, 3757-3765
-
(1982)
Biochemistry
, vol.21
, pp. 3757-3765
-
-
Stone, S.R.1
Morrison, J.F.2
-
30
-
-
0026005997
-
Transient intermediates in the folding of dihydrofolate reductase as detected by far-ultraviolet circular dichroism spectroscopy
-
Kuwajima, K., Garvey, E.P., Finn, B.E., Matthews, C.R., and Sugai, S. (1991) Transient intermediates in the folding of dihydrofolate reductase as detected by far-ultraviolet circular dichroism spectroscopy. Biochemistry 30, 7693-7703
-
(1991)
Biochemistry
, vol.30
, pp. 7693-7703
-
-
Kuwajima, K.1
Garvey, E.P.2
Finn, B.E.3
Matthews, C.R.4
Sugai, S.5
-
31
-
-
0023055739
-
Folding of dihydrofolate reductase from Escherichia coli
-
Touchette, N.A., Perry, K.M., and Matthews, C.R. (1986) Folding of dihydrofolate reductase from Escherichia coli. Biochemistry 25, 5445-5452
-
(1986)
Biochemistry
, vol.25
, pp. 5445-5452
-
-
Touchette, N.A.1
Perry, K.M.2
Matthews, C.R.3
-
32
-
-
0023240190
-
Effect for single amino acid replacements on the folding and stability of dihydrofolate reductase from Escherichia coli
-
Perry, K.M., Onuffer, J.J., Touchette, N.A., Herndon, C.S., Gittelman, M.S., Matthews, C.R., Chen, J.T., Mayer, R.J., Taira, K., Benkovic, S.J., Howell, E.E., and Kraut, J. (1987) Effect for single amino acid replacements on the folding and stability of dihydrofolate reductase from Escherichia coli. Biochemistry 26, 2674-2682
-
(1987)
Biochemistry
, vol.26
, pp. 2674-2682
-
-
Perry, K.M.1
Onuffer, J.J.2
Touchette, N.A.3
Herndon, C.S.4
Gittelman, M.S.5
Matthews, C.R.6
Chen, J.T.7
Mayer, R.J.8
Taira, K.9
Benkovic, S.J.10
Howell, E.E.11
Kraut, J.12
-
33
-
-
0024963670
-
Effects of multiple replacements at a single position on the folding and stability of dihydrofolate reductase from Escherichia coli
-
Garvey, E.P. and Matthews, C.R. (1989) Effects of multiple replacements at a single position on the folding and stability of dihydrofolate reductase from Escherichia coli. Biochemistry 28, 2083-2093
-
(1989)
Biochemistry
, vol.28
, pp. 2083-2093
-
-
Garvey, E.P.1
Matthews, C.R.2
-
34
-
-
0027315969
-
A reexamination of the folding mechanism of dihydrofolate reductase from Escherichia coli: Verification and refinement of a four-channel model
-
Jennings, P.A., Finn, B.E., Jones, B.E., and Matthews, C.R. (1993) A reexamination of the folding mechanism of dihydrofolate reductase from Escherichia coli: Verification and refinement of a four-channel model. Biochemistry 32, 3783-3789
-
(1993)
Biochemistry
, vol.32
, pp. 3783-3789
-
-
Jennings, P.A.1
Finn, B.E.2
Jones, B.E.3
Matthews, C.R.4
-
35
-
-
0027715218
-
Collapse of parallel folding channels in dihydrofolate reductase from Escherichia coli by site-directed mutagenesis
-
Iwakura, M., Jones, B.E., Falzone, C.J., and Matthews, C.R. (1993) Collapse of parallel folding channels in dihydrofolate reductase from Escherichia coli by site-directed mutagenesis. Biochemistry 32, 13566-13574
-
(1993)
Biochemistry
, vol.32
, pp. 13566-13574
-
-
Iwakura, M.1
Jones, B.E.2
Falzone, C.J.3
Matthews, C.R.4
-
36
-
-
0026788011
-
Structure and function of alternative proton-relay mutants of dihydrofolate reductase
-
David, C.L., Howell, E.E., Farnum, M.F., Villafranca, J.E., Oatley, S.J., and Kraut, J. (1992) Structure and function of alternative proton-relay mutants of dihydrofolate reductase. Biochemistry 31, 9813-9822
-
(1992)
Biochemistry
, vol.31
, pp. 9813-9822
-
-
David, C.L.1
Howell, E.E.2
Farnum, M.F.3
Villafranca, J.E.4
Oatley, S.J.5
Kraut, J.6
-
37
-
-
0023668136
-
Probing the functional role of phenylalanine-31 of Escherichia coli dihydrofolate reductase by site-directed mutagenesis
-
Chen, J.-T., Taira, K., Tu, C.-P.D., and Benkovic, S.J. (1987) Probing the functional role of phenylalanine-31 of Escherichia coli dihydrofolate reductase by site-directed mutagenesis. Biochemistry 26, 4093-4100
-
(1987)
Biochemistry
, vol.26
, pp. 4093-4100
-
-
Chen, J.-T.1
Taira, K.2
Tu, C.-P.D.3
Benkovic, S.J.4
-
38
-
-
0023667702
-
Construction of an altered proton donation mechanism in Escherichia coli dihydrofolate reductase
-
Howell, E.E., Warren, M.S., Booth, C.L., Villafranca, J.E., and Kraut, J. (1987) Construction of an altered proton donation mechanism in Escherichia coli dihydrofolate reductase. Biochemistry 26, 8591-8598
-
(1987)
Biochemistry
, vol.26
, pp. 8591-8598
-
-
Howell, E.E.1
Warren, M.S.2
Booth, C.L.3
Villafranca, J.E.4
Kraut, J.5
-
39
-
-
0026347044
-
Investigation of the functional role of tryptophan-22 in Escherichia coli dihydrofolate reductase by site-directed mutagenesis
-
Warren, M.S., Brown, K.A., Farnum, M.F., Howell, E.E., and Kraut, J. (1991) Investigation of the functional role of tryptophan-22 in Escherichia coli dihydrofolate reductase by site-directed mutagenesis. Biochemistry 30, 11092-11103
-
(1991)
Biochemistry
, vol.30
, pp. 11092-11103
-
-
Warren, M.S.1
Brown, K.A.2
Farnum, M.F.3
Howell, E.E.4
Kraut, J.5
-
40
-
-
0021107943
-
Site-directed mutagenesis as a probe of enzyme structure and catalysis: Tyrosyl-tRNA synthetase cysteine-35 to glycine-35 mutation
-
Wilkinson, A.J., Fersht, A.R., Blow, D.M., and Winter, G. (1983) Site-directed mutagenesis as a probe of enzyme structure and catalysis: tyrosyl-tRNA synthetase cysteine-35 to glycine-35 mutation. Biochemistry 22, 3581-3586
-
(1983)
Biochemistry
, vol.22
, pp. 3581-3586
-
-
Wilkinson, A.J.1
Fersht, A.R.2
Blow, D.M.3
Winter, G.4
-
41
-
-
0025082684
-
Additivity of mutational effects in proteins
-
Wells, J.A. (1990) Additivity of mutational effects in proteins. Biochemistry 29, 8509-8517
-
(1990)
Biochemistry
, vol.29
, pp. 8509-8517
-
-
Wells, J.A.1
-
42
-
-
0015217634
-
The solubility of amino acids and two glycine peptides in aqueous ethanol and dioxane solutions
-
Nozaki, Y. and Tanford, C. (1971) The solubility of amino acids and two glycine peptides in aqueous ethanol and dioxane solutions. J. Biol. Chem. 246, 2211-2217
-
(1971)
J. Biol. Chem.
, vol.246
, pp. 2211-2217
-
-
Nozaki, Y.1
Tanford, C.2
-
43
-
-
0021195067
-
Amino acid, peptide, and protein volume in solution
-
Zamyatnin, A.A. (1984) Amino acid, peptide, and protein volume in solution. Annu. Rev. Biophys. Bioeng. 13, 145-165
-
(1984)
Annu. Rev. Biophys. Bioeng.
, vol.13
, pp. 145-165
-
-
Zamyatnin, A.A.1
-
44
-
-
0023645203
-
Interior and surface of monomeric proteins
-
Miller, S., Janin, J., Lesk, A.M., and Chothia, C. (1987) Interior and surface of monomeric proteins. J. Mol. Biol. 196, 641-656
-
(1987)
J. Mol. Biol.
, vol.196
, pp. 641-656
-
-
Miller, S.1
Janin, J.2
Lesk, A.M.3
Chothia, C.4
|