Monitoring of diguanylate cyclase activity and of cyclic-di-GMP biosynthesis by whole-cell assays suitable for high-throughput screening of biofilm inhibitors

Applied Microbiology and Biotechnology

Volumn 85, Issue 4, 2010, Pages 1095-1104

  Antoniani, Davide ^a   Bocci, Paola ^b   Maciąg, Anna ^a   Raffaelli, Nadia ^b   Landini, Paolo ^a  

^a UNIVERSITY OF MILAN   (Italy)

^b UNIVERSITÀ POLITECNICA DELLE MARCHE   (Italy)

Author keywords

Antimicrobial drugs; Biofilm formation; C di GMP; Diguanylate cyclase; High throughput screening; Sulfathiazole

Indexed keywords

ANTIMICROBIAL AGENTS; ASSAYS; AZO DYES; BIOCHEMISTRY; BIOFILMS; BIOSYNTHESIS; DRUG PRODUCTS; ENCODING (SYMBOLS); ESCHERICHIA COLI; GENE ENCODING; GENE EXPRESSION; MOLECULAR BIOLOGY; SIGNAL ENCODING;

ANTIMICROBIAL DRUG; BIOFILM FORMATION; CYCLASES; HIGH THROUGHPUT SCREENING; SULFATHIAZOLE;

THROUGHPUT;

ADRA PROTEIN; BACTERIAL PROTEIN; BIS (3',5') CYCLIC DIGUANYLIC ACID; CONGO RED; DIGUANYLATE CYCLASE; ENZYME INHIBITOR; GUANOSINE PHOSPHATE; GUANYLATE CYCLASE; SULFATHIAZOLE; UNCLASSIFIED DRUG;

ANTIMICROBIAL ACTIVITY; BACTERIUM; BIOFILM; DRUG; ENZYME ACTIVITY; GENE EXPRESSION; METABOLISM; POLYSACCHARIDE; PROTEIN;

ARTICLE; BACTERIAL GENE; BIOFILM; CULTURE MEDIUM; ENZYME ACTIVITY; ENZYME BINDING; ENZYME INHIBITION; ENZYME METABOLISM; ESCHERICHIA COLI; GENE ACTIVITY; GENE LIBRARY; GENE OVEREXPRESSION; HIGH THROUGHPUT SCREENING; INHIBITION KINETICS; MICROTITER PLATE ASSAY; NONHUMAN; NUCLEOTIDE METABOLISM; PHENOTYPE; REPORTER GENE;

ESCHERICHIA COLI; NEGIBACTERIA;

EID: 76649134086     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00253-009-2199-x     Document Type: Article

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