Quantitative determination of cyclic diguanosine monophosphate concentrations in nucleotide extracts of bacteria by matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry

Analytical Biochemistry

Volumn 386, Issue 1, 2009, Pages 53-58

  Simm, Roger ^a   Morr, Michael ^b   Remminghorst, Uwe ^a   Andersson, Mats ^a   Römling, Ute ^a  

^a KAROLINSKA INSTITUTE   (Sweden)

^b HELMHOLTZ CENTRE FOR INFECTION RESEARCH   (Germany)

Author keywords

c di GMP; HPLC; MALDI TOF; Quantification

Indexed keywords

BIOCHEMISTRY; CELL SIGNALING; ESCHERICHIA COLI; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; PHYSIOLOGICAL MODELS; SALMONELLA;

HPLC; MALDI-TOF; MATRIX-ASSISTED LASER DESORPTION/IONIZATION-TIME-OF-FLIGHT MASS SPECTROMETRY; PHYSIOLOGICAL RESPONSE; PSEUDOMONAS AERUGINOSA; QUANTIFICATION; QUANTITATIVE DETERMINATIONS; SALMONELLA TYPHIMURIUM;

C (PROGRAMMING LANGUAGE);

CYCLIC DIGUANOSINE MONOPHOSPHATE; GUANOSINE DERIVATIVE; NUCLEOTIDE; UNCLASSIFIED DRUG;

ANALYTIC METHOD; ARTICLE; BACTERIAL CELL; CONCENTRATION (PARAMETERS); ESCHERICHIA COLI; GROWTH CURVE; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; NONHUMAN; PRIORITY JOURNAL; PSEUDOMONAS AERUGINOSA; QUANTITATIVE ANALYSIS; SALMONELLA TYPHIMURIUM; SIGNAL TRANSDUCTION; VIBRIO CHOLERAE;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; PSEUDOMONAS AERUGINOSA; SALMONELLA TYPHIMURIUM; VIBRIO CHOLERAE;

EID: 58749088368     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2008.12.013     Document Type: Article

References (22)

1. Römling U., Gomelsky M., and Galperin M.Y. C-di-GMP: the dawning of a novel bacterial signalling system. Mol. Microbiol. 57 (2005) 629-639
2. Galperin M.Y. A census of membrane-bound and intracellular signal transduction proteins in bacteria: bacterial IQ, extroverts, and introverts. BMC Microbiol. 5 (2005) 35
3. Paul R., Weiser S., Amiot N.C., Chan C., Schirmer T., Giese B., and Jenal U. Cell cycle-dependent dynamic localization of a bacterial response regulator with a novel di-guanylate cyclase output domain. Genes Dev. 18 (2004) 715-727
4. Schmidt A.J., Ryjenkov D.A., and Gomelsky M. The ubiquitous protein domain EAL is a cyclic diguanylate-specific phosphodiesterase: enzymatically active and inactive EAL domains. J. Bacteriol. 187 (2005) 4774-4781
5. Ryan R.P., Fouhy Y., Lucey J.F., Crossman L.C., Spiro S., He Y.W., Zhang L.H., Heeb S., Camara M., Williams P., and Dow J.M. Cell-cell signaling in Xanthomonas campestris involves an HD-GYP domain protein that functions in cyclic di-GMP turnover. Proc. Natl. Acad. Sci. USA 103 (2006) 6712-6717
6. Simm R., Morr M., Kader A., Nimtz M., and Römling U. GGDEF and EAL domains inversely regulate cyclic di-GMP levels and transition from sessility to motility. Mol. Microbiol. 53 (2004) 1123-1134
7. Tischler A.D., and Camilli A. Cyclic diguanylate (c-di-GMP) regulates Vibrio cholerae biofilm formation. Mol. Microbiol. 53 (2004) 857-869
8. Bochner B.R., and Ames B.N. Complete analysis of cellular nucleotides by two-dimensional thin layer chromatography. J. Biol. Chem. 257 (1982) 9759-9769
9. Vorm O., and Roepstorff P. Peptide sequence information derived by partial acid hydrolysis and matrix-assisted laser desorption/ionization mass spectrometry. Biol. Mass Spectrom. 23 (1994) 734-740
10. Hsu C.Y., and Dennis D. RNA polymerase: linear competitive inhibition by bis-(3′ to 5′)-cyclic dinucleotides, NpNp. Nucleic Acids Res. 10 (1982) 5637-5647
11. de Vroom E., van der Marel G.A., and van Boom J.H. Synthesis of ribonucleoside 3′, 5′-cyclic phosphorothioates using a modified hydroxybenzotriazole phosphotriester approach. Recl. Trav. Chim. Pays-Bas 106 (1987) 577-580
12. Römling U., Sierralta W.D., Eriksson K., and Normark S. Multicellular and aggregative behavior of Salmonella typhimurium strains is controlled by mutations in the agfD promoter. Mol. Microbiol. 28 (1998) 249-264
13. Römling U., and Amikam D. Cyclic di-GMP as a second messenger. Curr. Opin. Microbiol. 9 (2006) 218-228
14. Kader A., Simm R., Gerstel U., Morr M., and Römling U. Hierarchical involvement of various GGDEF domain proteins in rdar morphotype development of Salmonella enterica serovar Typhimurium. Mol. Microbiol. 60 (2006) 602-616
15. Kulesekara H., Lee V., Brencic A., Liberati N., Urbach J., Miyata S., Lee D.G., Neely A.N., Hyodo M., Hayakawa Y., Ausubel F.M., and Lory S. Analysis of Pseudomonas aeruginosa diguanylate cyclases and phosphodiesterases reveals a role for bis-(3′-5′)-cyclic-GMP in virulence. Proc. Natl. Acad. Sci. USA 103 (2006) 2839-2844
16. Witte G., Hartung S., Buttner K., and Hopfner K.P. Structural biochemistry of a bacterial checkpoint protein reveals diadenylate cyclase activity regulated by DNA recombination intermediates. Mol. Cell 30 (2008) 167-178
17. Simm R., Lusch A., Kader A., Andersson M., and Römling U. Role of EAL-containing proteins in multicellular behavior of Salmonella enterica serovar Typhimurium. J. Bacteriol. 189 (2007) 3613-3623
18. Ilic B., Craighead H.G., Krylov S., Senaratne W., Ober C., and Neuzil P. Attogram detection using nanoelectromechanical oscillators. J. Appl. Phys. 95 (2004) 3694-3703
19. Opheim D., and Bernlohr R.W. Purification and regulation of glucose-6-phosphate dehydrogenase from Bacillus licheniformis. J. Bacteriol. 116 (1973) 1150-1159
20. Jonas K., Edwards A.N., Simm R., Romeo T., Römling U., and Melefors O. The RNA binding protein CsrA controls c-di-GMP metabolism by directly regulating the expression of GGDEF proteins. Mol. Microbiol. 70 (2008) 236-257
21. Meissner A., Wild V., Simm R., Rohde M., Erck C., Bredenbruch F., Morr M., Römling U., and Häussler S. Pseudomonas aeruginosa cupA-encoded fimbriae expression is regulated by a GGDEF and EAL domain-dependent modulation of the intracellular level of cyclic diguanylate. Environ. Microbiol. 9 (2007) 2475-2485
22. Römling U., Bian Z., Hammar M., Sierralta W.D., and Normark S. Curli fibers are highly conserved between Salmonella typhimurium and Escherichia coli with respect to operon structure and regulation. J. Bacteriol. 180 (1998) 722-731