Effects of acetic acid and its assimilation in fed-batch cultures of recombinant Escherichia coli containing human-like collagen cDNA

Journal of Bioscience and Bioengineering

Volumn 109, Issue 3, 2010, Pages 257-261

  Xue, Wenjiao ^a   Fan, Daidi ^a,c   Shang, Longan ^b,c   Zhu, Chenhui ^a,c   Ma, Xiaoxuan ^a,c   Zhu, Xiaoli ^a,c   Yu, Yuanyuan ^a  

^a NORTHWEST UNIVERSITY   (China)

^b ZHEJIANG UNIVERSITY   (China)

^c Shaanxi R and D Center of Biomaterials and Fermentation Engineering   (China)

Author keywords

Acetic acid; Assimilation; Escherichia coli; Fed batch culture; Glucose starvation; Human like collagen

Indexed keywords

ACETIC ACID CONCENTRATION; BATCH CULTURE; CULTURE MEDIA; E. COLI; FED-BATCH CULTURES; FERMENTATION PROCESS; HUMAN LIKE COLLAGEN; INHIBITORY EFFECT; PRIMARY PROCESSING; RECOMBINANT E. COLI; RECOMBINANT ESCHERICHIA COLI; RECOMBINANT PROTEIN PRODUCTIONS; SCALING-UP; SYSTEMATIC STUDY;

ACETIC ACID; ACIDS; CELL CULTURE; CELL GROWTH; COLLAGEN; ESCHERICHIA COLI; FERMENTATION; GENE EXPRESSION; GLUCOSE; GROWTH KINETICS; METAL ANALYSIS; PH EFFECTS;

BATCH CELL CULTURE;

ACETIC ACID; COLLAGEN; COMPLEMENTARY DNA; GLUCOSE; RECOMBINANT PROTEIN;

ARTICLE; BACTERIUM CULTURE; CELL GROWTH; CULTURE MEDIUM; ESCHERICHIA COLI; FED BATCH CULTURE; FERMENTATION; NONHUMAN; STARVATION;

ACETIC ACID; CELL CULTURE TECHNIQUES; COLLAGEN; DNA, COMPLEMENTARY; ESCHERICHIA COLI; GENETIC ENGINEERING; HUMANS; RECOMBINATION, GENETIC;

ESCHERICHIA COLI;

EID: 76049106890     PISSN: 13891723     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jbiosc.2009.08.008     Document Type: Article

References (26)

1. Eiteman M.A., and Altman E. Overcoming acetate in Escherichia coli recombinant protein fermentations. Trends Biotechnol. 24 (2006) 530-536
2. De Mey M., De Maeseneire S., Soetaert W., and Vandamme E. Minimizing acetate formation in E. coli fermentations. J. Ind. Microbiol. Biotechnol. 34 (2007) 689-700
3. Johnston W., Cord-Ruwisch R., and Cooney M.J. Industrial control of recombinant E. coli fed-batch culture: new perspectives on traditional controlled variables. Bioprocess Biosyst. Eng. 25 (2002) 111-120
4. Lee S.Y. High cell-density culture of Escherichia coli. Trends Biotechnol. 14 (1996) 98-105
5. Turner C., Gregory M.E., and Turner M.K. A study of the effect of specific growth rate and acetate on recombinant protein production of Escherichia coli JM107. Biotechnol. Lett. 16 (1994) 891-896
6. Xu B., Jahic M., Blomsten G., and Enfors S.-O. Glucose overflow metabolism and mixed-acid fermentation in aerobic large-scale fed-batch processes with Escherichia coli. Appl. Microbiol. Biotechnol. 51 (1999) 564-571
7. Ko Y.-F., Bentley W.E., and Weigand W.A. An integrated metabolic modeling approach to describe the energy efficiency of Escherichia coli fermentations under oxygen-limited conditions: cellular energetics, carbon flux, and acetate production. Biotechnol. Bioeng. 42 (1993) 843-853
8. Faulkner E., Barrett M., Okor S., Kieran P., Casey E., Paradisi F., Engel P., and Glennon B. Use of fed-batch cultivation for achieving high cell densities for the pilot-scale production of a recombinant protein (phenylalanine dehydrogenase) in Escherichia coli. Biotechnol. Prog. 22 (2006) 889-897
9. Luli G.W., and Strohl W.R. Comparison of growth, acetate production, and acetate inhibition of Escherichia coli strains in batch and fed-batch fermentations. Appl. Environ. Microbiol. 56 (1990) 1004-1011
10. Kleman G.L., and Strohl W.R. Acetate metabolism by Escherichia coli in high-cell-density fermentation. Appl. Environ. Microbiol. 60 (1994) 3952-3958
11. Nakano K., Rischke M., Sato S., and Märkl H. Influence of acetic acid on the growth of Escherichia coli K12 during high-cell-density cultivation in a dialysis reactor. Appl. Microbiol. Biotechnol. 48 (1997) 597-601
12. Fuchs C., Köster D., Wiebusch S., Mahr K., Eisbrenner G., and Märkl H. Scale-up of dialysis fermentation for high cell density cultivation of Escherichia coli. J. Biotechnol. 93 (2002) 243-251
13. Chen X.A., Cen P.L., and Chen J.S. Enhanced production of human epidermal growth factor by a recombinant Escherichia coli integrated with in situ exchange of acetic acid by macroporous ion-exchange resin. J. Biosci. Bioeng. 100 (2005) 579-581
14. Wolfe A.J. The acetate switch. Microbiol. Mol. Biol. Rev. 69 (2005) 12-50
15. Castan A., Näsman A., and Enfors S.O. Oxygen enriched air supply in Escherichia coli processes: production of biomass and recombinant human growth hormone. Enzyme Microb. Technol. 30 (2002) 847-854
16. Suárez D.C., and Kilikian B.V. Acetic acid accumulation in aerobic growth of recombinant Escherichia coli. Process Biochem. 35 (2000) 1051-1055
17. Luo Y.E., Fan D.D., Ma X.X., Wang D.W., Mi Y., Hua X.F., and Li W.H. Process control for production of human-like collagen in fed-batch culture of Escherichia coli BL21. Chin. J. Chem. Eng. 13 (2005) 276-279
18. Fan D.D., Luo Y.E., Mi Y., Ma X.X., and Shang L.A. Characteristics of fed-batch cultures of recombinant Escherichia coli containing human-like collagen cDNA at different specific growth rates. Biotechnol. Lett. 27 (2005) 865-870
19. Hua X.F., Fan D.D., Luo Y.E., Zhang X., Shi H.J., Mi Y., Ma X.X., Shang L.A., and Zhao G.F. Kinetics of high cell density fed-batch culture of recombinant Escherichia coli producing human-like collagen. Chin. J. Chem. Eng. 14 (2006) 242-247
20. Singer V.L., Jones L.J., Yue S.T., and Haugland R.P. Characterization of PicoGreen reagent and development of a fluorescence-based solution assay for double-stranded DNA quantification. Anal. Biochem. 249 (1997) 228-238
21. Castan A., and Enfors S.-O. Characteristics of a DO-controlled fed-batch culture of Escherichia coli. Bioprocess Eng. 22 (2000) 509-515
22. Lin H.Y., Mathiszik B., Xu B., Enfors S.-O., and Neubauer P. Determination of the maximum specific uptake capacities for glucose and oxygen in glucose-limited fed-batch cultivations of Escherichia coli. Biotechnol. Bioeng. 73 (2001) 347-357
23. Nancib N., Branlant C., and Boudrant J. Metabolic roles of peptone and yeast extract for the culture of a recombinant strain of Escherichia coli. J. Ind. Microbiol. 8 (1991) 165-169
24. Luo Y.E., Fan D.D., Shang L.A., Shi H.J., Ma X.X., Mi Y., and Zhao G.F. Analysis of metabolic flux in Escherichia coli expressing human-like collagen in fed-batch culture. Biotechnol. Lett. 30 (2008) 637-643
25. Lischke H.H., Brandes L., Wu X., and Schügerl K. Influence of acetate on the growth of recombinant Escherichia coli JM103 and product formation. Bioprocess Eng. 9 (1993) 155-157
26. Gschaedler A., Robas N., Boudrant J., and Branlant C. Effects of pulse addition of carbon sources on continuous cultivation of Escherichia coli containing a recombinant E. coli gapA gene. Biotechnol. Bioeng. 63 (1999) 712-720