Directed evolution of chemotaxis inhibitory protein of Staphylococcus aureus generates biologically functional variants with reduced interaction with human antibodies

Protein Engineering, Design and Selection

Volumn 23, Issue 2, 2010, Pages 91-101

  Gustafsson, Erika ^a,b   Rosén, Anna ^a   Barchan, Karin ^a   Van Kessel, Kok P M ^d   Haraldsson, Karin ^a   Lindman, Stina ^b   Forsberg, Cecilia ^a   Ljung, Lill ^a   Bryder, Karin ^a   Walse, Björn ^c   Haas, Pieter Jan ^d   Van Strijp, Jos A G ^d   Furebring, Christina ^a  

^a Alligator Bioscience AB   (Sweden)

^b LUND UNIVERSITY   (Sweden)

^c SAomics AB   (Sweden)

^d UNIVERSITY MEDICAL CENTER UTRECHT   (Netherlands)

Author keywords

Antibodies; Directed evolution; Inflammation; Phage selection

Indexed keywords

AMINO ACID SUBSTITUTION; ANTI-INFLAMMATORY DRUGS; BIOLOGICAL FUNCTIONS; BLOCKING CAPACITY; DIRECTED EVOLUTION; DNA RECOMBINATION; HUMAN ANTIBODIES; HUMAN IGG; IMMUNE CELLS; INFLAMMATION; INHIBITORY PROTEINS; PEPTIDE RECEPTOR; PHAGE SELECTION; RANDOM MUTAGENESIS; RATIONAL DESIGN; STAPHYLOCOCCUS AUREUS;

AMINO ACIDS; BIOASSAY; BIOCHEMISTRY; BIOCHIPS; ORGANIC ACIDS; PATHOLOGY;

ANTIBODIES;

ANTIBODY; BACTERIAL PROTEIN; CHEMOTAXIS INHIBITORY PROTEIN OF STAPHYLOCOCCUS AUREUS; COMPLEMENT COMPONENT C5A RECEPTOR; FORMYLPEPTIDE RECEPTOR; IMMUNOGLOBULIN G; UNCLASSIFIED DRUG;

AMINO ACID SUBSTITUTION; ARTICLE; BACTERIAL GENE; BACTERIOPHAGE; CHIPS GENE; DNA RECOMBINATION; ENZYME LINKED IMMUNOSORBENT ASSAY; EVOLUTION; GENE MUTATION; HUMAN; HUMAN CELL; IMMUNOCOMPETENT CELL; INFLAMMATION; MUTAGENESIS; NONHUMAN; PRIORITY JOURNAL; PROTEIN PROTEIN INTERACTION; PROTEIN VARIANT; STAPHYLOCOCCUS AUREUS;

AMINO ACID SEQUENCE; BACTERIAL PROTEINS; CELL LINE; DIRECTED MOLECULAR EVOLUTION; DRUG DESIGN; GENE EXPRESSION; HUMANS; IMMUNOGLOBULIN G; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; NEUTROPHILS; RECEPTOR, ANAPHYLATOXIN C5A; RECOMBINANT PROTEINS; SEQUENCE ALIGNMENT; STAPHYLOCOCCUS AUREUS;

STAPHYLOCOCCUS AUREUS;

EID: 75649131490     PISSN: 17410126     EISSN: 17410134     Source Type: Journal    
DOI: 10.1093/protein/gzp062     Document Type: Article

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