Limited mutagenesis increases the stability of human carboxypeptidase U (TAFIa) and demonstrates the importance of CPU stability over proCPU concentration in down-regulating fibrinolysis

FEBS Journal

Volumn 273, Issue 4, 2006, Pages 778-792

  Knecht, Wolfgang ^a   Willemse, Johan ^c   Stenhamre, Hanna ^a   Andersson, Mats ^b   Berntsson, Pia ^a   Furebring, Christina ^b   Harrysson, Anna ^a   Hager, Ann Christin Malmborg ^b   Wissing, Britt Marie ^a   Hendriks, Dirk ^c   Cronet, Philippe ^a  

^a ASTRAZENECA R AND D   (Sweden)

^b Alligator Bioscience AB   (Sweden)

^c UNIVERSITY OF ANTWERP   (Belgium)

Author keywords

Carboxypeptidase; Coagulation; Directed evolution; Fibrinolysis; Protease

Indexed keywords

MUTANT PROTEIN; THROMBIN ACTIVATABLE FIBRINOLYSIS INHIBITOR; TRYPSIN;

ANIMAL CELL; ARTICLE; CONTROLLED STUDY; DOWN REGULATION; DRUG SCREENING; ENZYME ACTIVITY; ENZYME STABILITY; FIBRINOLYSIS; MOLECULAR MECHANICS; MOUSE; MUTAGENESIS; MUTATION; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; TEMPERATURE DEPENDENCE; THERMOSTABILITY;

AMINO ACID SEQUENCE; ANIMALS; BLOOD COAGULATION; CARBOXYPEPTIDASE U; CELL LINE; DOWN-REGULATION; ENZYME ACTIVATION; ENZYME STABILITY; FIBRIN; FIBRINOLYSIS; HEAT; HUMANS; LYSINE; MOLECULAR SEQUENCE DATA; MUTAGENESIS; POINT MUTATION; PROTEIN DENATURATION; PROTEIN PRECURSORS; SEQUENCE ALIGNMENT;

ANIMALIA;

EID: 33644952604     PISSN: 1742464X     EISSN: 17424658     Source Type: Journal    
DOI: 10.1111/j.1742-4658.2006.05110.x     Document Type: Article

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