Nucleosomes are depleted at the VSG expression site transcribed by RNA polymerase I in African trypanosomes

Eukaryotic Cell

Volumn 9, Issue 1, 2010, Pages 148-154

  Figueiredo, Luisa M ^a   Cross, George A M ^a  

^a ROCKEFELLER UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DNA DIRECTED RNA POLYMERASE; HISTONE; VARIANT SURFACE GLYCOPROTEIN;

ARTICLE; CHEMISTRY; CHROMATIN; CONFORMATION; GENE EXPRESSION REGULATION; GENETIC TRANSCRIPTION; GENETICS; METABOLISM; NUCLEOSOME; TRYPANOSOMA BRUCEI;

CHROMATIN; GENE EXPRESSION REGULATION; HISTONES; NUCLEIC ACID CONFORMATION; NUCLEOSOMES; RNA POLYMERASE I; TRANSCRIPTION, GENETIC; TRYPANOSOMA BRUCEI BRUCEI; VARIANT SURFACE GLYCOPROTEINS, TRYPANOSOMA;

EUKARYOTA; TRYPANOSOMA;

EID: 75649110809     PISSN: 15359778     EISSN: None     Source Type: Journal    
DOI: 10.1128/EC.00282-09     Document Type: Article

References (34)

1. Alsford, S., T. Kawahara, L. Glover, and D. Horn. 2005. Tagging a T. brucei RRNA locus improves stable transfection efficiency and circumvents inducible expression position effects. Mol. Biochem. Parasitol. 144:142-148.
2. Boothroyd, C. E., et al. 2009. A yeast-endonuclease-generated DNA break induces antigenic switching in Trypanosoma brucei. Nature 459:278-281.
3. Brandenburg, J., et al. 2007. Multifunctional class I transcription in Trypano-soma brucei depends on a novel protein complex. EMBO J. 26:4856-4866.
4. Dammann, R., R. Lucchini, T. Koller, and J. M. Sogo. 1993. Chromatin structures and transcription of rDNA in yeast Saccharomyces cerevisiae. Nucleic Acids Res. 21:2331-2338.
5. Dreesen, O., B. Li, and G. A. M. Cross. 2007. Telomere structure and function in trypanosomes: a proposal. Nat. Rev. Microbiol. 5:70-75.
6. Elgin, S. C., and S. I. Grewal. 2003. Heterochromatin: silence is golden. Curr. Biol. 13:R895-R898.
7. Figueiredo, L. M., G. A. M. Cross, and C. J. Janzen. 2009. Epigenetic regulation in African trypanosomes: a new kid on the block. Nat. Rev. Microbiol. 7:504-513.
8. Figueiredo, L. M., C. J. Janzen, and G. A. M. Cross. 2008. A histone methyltransferase modulates antigenic variation in African trypanosomes. PLoS Biol. 6:e161.
9. Greaves, D. R., and P. Borst. 1987. Trypanosoma brucei variant-specific glycoprotein gene chromatin is sensitive to single-strand-specific endonuclease digestion. J. Mol. Biol. 197:471-483.
10. Hertz-Fowler, C., et al. 2008. Telomeric expression sites are highly conserved in Trypanosoma brucei. PLoS One 3:e3527.
11. Hirumi, H., and K. Hirumi. 1989. Continuous cultivation of Trypanosoma brucei blood stream forms in a medium containing a low concentration of serum protein without feeder cell layers. J. Parasitol. 75:985-989.
12. Hitchcock, R. A., S. Thomas, D. A. Campbell, and N. R. Sturm. 2007. The promoter and transcribed regions of the Leishmania tarentolae spliced leader RNA gene array are devoid of nucleosomes. BMC Microbiol. 7:44.
13. Hughes, K., et al. 2007. A novel ISWI is involved in VSG expression site downregulation in African trypanosomes. EMBO J. 26:2400-2410.
14. Johnson, J. G., and G. A. M. Cross. 1979. Selective cleavage of variant surface glycoproteins from Trypanosoma brucei. Biochem. J. 178:689-697.
15. Jones, H. S., et al. 2007. RNA polymerase I in yeast transcribes dynamic nucleosomal rDNA. Nat. Struct. Mol. Biol. 14:123-130.
16. Lee, C. K., Y. Shibata, B. Rao, B. D. Strahl, and J. D. Lieb. 2004. Evidence for nucleosome depletion at active regulatory regions genome-wide. Nat. Genet. 36:900-905.
17. Li, B., M. Carey, and J. L. Workman. 2007. The role of chromatin during transcription. Cell 128:707-719.
18. Lowell, J. E., and G. A. M. Cross. 2004. A variant histone H3 is enriched at telomeres in Trypanosoma brucei. J. Cell Sci. 117:5937-5947.
19. Lowell, J. E., F. Kaiser, C. J. Janzen, and G. A. M. Cross. 2005. Histone H2AZ dimerizes with a novel variant H2B and is enriched at repetitive DNA in Trypanosoma brucei. J. Cell Sci. 118:5721-5730.
20. Mandava, V., et al. 2007. Histone modifications in Trypanosoma brucei. Mol. Biochem. Parasitol. 156:41-50.
21. McAndrew, M., S. Graham, C. Hartmann, and C. Clayton. 1998. Testing promoter activity in the trypanosome genome: isolation of a metacyclic-type VSG promoter, and unexpected insights into RNA polymerase II transcription. Exp. Parasitol. 90:65-76.
22. McStay, B., and I. Grummt. 2008. The epigenetics of rRNA genes: from molecular to chromosome biology. Annu. Rev. Cell Dev. Biol. 24:131-157.
23. Merz, K., et al. 2008. Actively transcribed rRNA genes in S. cerevisiae are organized in a specialized chromatin associated with the high-mobility group protein Hmo1 and are largely devoid of histone molecules. Genes Dev. 22:1190-1204.
24. Nagy, P. L., M. L. Cleary, P. O. Brown, and J. D. Lieb. 2003. Genomewide demarcation of RNA polymerase II transcription units revealed by physical fractionation of chromatin. Proc. Natl. Acad. Sci. U.S.A. 100:6364-6369.
25. Navarro, M., and G. A. M. Cross. 1998. In situ analysis of a variant surface glycoprotein expression-site promoter region in Trypanosoma brucei. Mol. Biochem. Parasitol. 94:53-66.
26. Navarro, M., and K. Gull. 2001. A Pol I transcriptional body associated with VSG mono-allelic expression in Trypanosoma brucei. Nature 414:759-763.
27. Pays, E., M. Lheureux, and M. Steinert. 1981. The expression-linked copy of a surface antigen gene in Trypanosoma is probably the one transcribed. Nature 292:265-267.
28. Siegel, T. N., et al. 2009. Four histone variants mark the boundaries of polycistronic transcription units in Trypanosoma brucei. Genes Dev. 23:1063-1076.
29. Stanne, T. M., and G. Rudenko. 2010. Active VSG expression sites in Trypanosoma brucei are depleted of nucleosomes. Eukaryot. Cell 9:136-147.
30. Stockdale, C., M. R. Swiderski, J. D. Barry, and R. McCulloch. 2008. Antigenic variation in Trypanosoma brucei: joining the DOTs. PLoS Biol. 6:e185.
31. Tongaonkar, P., et al. 2005. Histones are required for transcription of yeast rRNA genes by RNA polymerase I. Proc. Natl. Acad. Sci. U.S.A. 102: 10129-10134.
32. Vanhamme, L., et al. 2000. Differential RNA elongation controls the variant surface glycoprotein gene expression sites of Trypanosoma brucei. Mol. Microbiol. 36:328-340.
33. Yang, X., L. M. Figueiredo, A. Espinal, E. Okubo, and B. Li. 2009. RAP1 is essential for silencing telomeric Variant Surface Glycoprotein genes in Trypanosoma brucei. Cell 137:99-109.
34. Yuan, X., W. Feng, A. Imhof, I. Grummt, and Y. Zhou. 2007. Activation of RNA polymerase I transcription by cockayne syndrome group B protein and histone methyltransferase G9a. Mol. Cell 27:585-595.