In situ analysis of a variant surface glycoprotein expression-site promoter region in Trypanosoma brucei

Molecular and Biochemical Parasitology

Volumn 94, Issue 1, 1998, Pages 53-66

  Navarro, Miguel ^a   Cross, George A M ^a  

^a ROCKEFELLER UNIVERSITY   (United States)

Author keywords

Antigenic variation; Expression site promoter; Nuclease hypersensitive sites; Trypanosoma brucei; Variant surface glycoprotein expression site

Indexed keywords

NUCLEASE; VARIANT SURFACE GLYCOPROTEIN;

ANTIGENIC VARIATION; ARTICLE; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; TRYPANOSOMA BRUCEI;

TRYPANOSOMA BRUCEI;

EID: 0032128354     PISSN: 01666851     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0166-6851(98)00049-8     Document Type: Article

References (51)

1. [1] de Lange T, Borst P. Genomic environment of the expression-linked extra copies of genes for surface antigens of Trypanosoma brucei resembles the end of a chromosome. Nature 1982;299:451-3.
2. [2] Pays E, Lheureux M, Steinert M. The expression linked copy of surface antigen gene in Trypanosoma brucei is probably the one transcribed. Nature 1981;292:265-7.
3. [3] Cross GAM. Antigenic variation in trypanosomes: secrets surface slowly. Bioessays 1996;18:283-91.
4. [4] Cross GAM. Cellular and genetic aspects of antigenic variation in trypanosomes. Annu Rev Immunol 1990;8:83-110.
5. [5] Vanhamme L, Pays E. Control of gene expression in trypanosomes. Microbiol Rev 1995;59:223-40.
6. [6] Cross GAM. Identification, purification and properties of variant-specific glycoprotein antigens constituting the surface coat of Trypanosoma brucei. Parasitology 1975;71:393-417.
7. [7] Borst P, Gommers-Ampt JH, Ligtenberg MJL, Rudenko G, Kieft R, Taylor MC, Blundell PA, van Leeuwen F. Control of antigenic variation in African trypanosomes. Cold Spring Harbor Symp Quant Biol 1993;58:105-14.
8. [8] Kooter JM, van der Spek HJ, Wagter R, d'Oliveira CE, van der Hoeven F, Johnson PJ, Borst P. The anatomy and transcription of a telomeric expression site for variant-specific surface antigens in T. brucei. Cell 1987;51:261-72.
9. [9] Johnson PJ, Kooter JM, Borst P. Inactivation of transcription by UV irradiation of T. brucei provides evidence for a multicistronic transcription unit including a VSG gene. Cell 1987;51:273-81.
10. [10] Zomerdijk JCBM, Kieft R, Shiels PG, Borst P. Alpha-amanitin-resistant transcription units in trypanosomes: A comparison of promoter sequences for a VSG gene expression site and for the ribosomal RNA genes. Nucleic Acids Res 1991;19:5153-8.
11. [11] Pays E, Coquelet H, Tebabi P, Pays A, Jefferies D, Steinert M, Koenig E, Williams RO, Roditi I. Trypanosoma brucei: Constitutive activity of the VSG and procyclin gene promoters. EMBO J 1990;9:3145-51.
12. [12] Gottesdiener K, Chung H-M, Brown SD, Lee MG-S, van der Ploeg LHT. Characterization of VSG gene expression site promoters and promoter-associated DNA rearrangement events. Mol Cell Biol 1991;11:2467-80.
13. [13] Cully DF, Ip HS, Cross GA. Coordinate transcription of variant surface glycoprotein genes and an expression site associated gene family in Trypanosoma brucei. Cell 1985;42:173-82.
14. [14] Zomerdijk JCBM, Ouellete M, ten Asbroek ALMA, Kieft R, Bommer AMM, Clayton CE, Borst P. The promoter for a variant surface glycoprotein gene expression site in Trypanosoma brucei. EMBO J 1990;9:2791-801.
15. [15] Navarro M, Cross GAM. DNA rearrangements associated with multiple consecutive directed antigenic switches in Trypanosoma brucei. Mol Cell Biol 1996;16:3615-25.
16. [16] Kooter JM, Borst P. Alpha-amanitin-insensitive transcription of variant surface glycoprotein genes provides further evidence for discontinuous transcription in trypanosomes. Nucleic Acids Res 1984;12:9457-72.
17. [17] Rudenko G, Bishop D, Gottesdiener K, van der Ploeg LHT. Alpha-amanitin resistant transcription of protein coding genes in insect and bloodstream form Trypanosoma brucei. EMBO J 1989;8:4259-63.
18. [18] Rudenko G, Lee MG-S, van der Ploeg LHT. The PARP and VSG genes of Trypanosoma brucei do not resemble RNA polymerase II transcription units in sensitivity to Sarkosyl in nuclear run-on assays. Nucleic Acids Res 1992;20:303-6.
19. [19] Gross DS, Garrard WT. Nuclease hypersensitive sites in chromatin. Annu Rev Biochem 1988;57:159-97.
20. [20] Greaves DR, Borst P. Trypanosoma brucei variant-specific glycoprotein gene chromatin is sensitive to single-strand-specific endonuclease digestion. J Mol Biol 1987;197:471-83.
21. [21] Vanhamme L, Pays A, Tebabi P, Alexandre S, Pays E. Specific binding of proteins to the noncoding strand of a crucial element of the variant surface glycoprotein, procyclin, and ribosomal promoters of Trypanosoma brucei. Mol Cell Biol 1995;15:5598-606.
22. [22] Pham VP, Qi CC, Gottesdiener KM. A detailed mutational analysis of the VSG gene expression site promoter. Mol Biochem Parasitol 1996;75:241-54.
23. [23] Cully DF, Gibbs CP, Cross GAM. Identification of proteins encoded by variant surface glycoprotein expression site-associated genes in Trypanosoma brucei. Mol Biochem Parasitol 1986;21:189-97.
24. [24] Florent I, Baltz T, Raibaud A, Eisen H. On the role of repeated sequences 5′ to variant surface glycoprotein genes in African trypanosomes. Gene 1987;53:55-62.
25. [25] Murphy NB, Guyaux M, Pays E, Steinert M. Analysis of VSG expression site sequence in T. brucei. UCLA Symposium on Molecular Strategies of Parasite Invasion, New Series vol. 1987;42:449-469.
26. [26] Alexandre S, Guyaux M, Murphy NB, Coquelet H, Pays A, Steinert M, Pays E. Putative genes of a variant-specific antigen gene transcription unit in Trypanosoma brucei. Mol Cell Biol 1988;8:2367-78.
27. [27] Michels PAM, van der Ploeg LHT, Liu AYC, Borst P. The inactivation and reactivation of an expression-linked gene copy for a variant surface glycoprotein in Trypanosoma brucei. EMBO J 1984;3:1345-51.
28. [28] Michels PAM, Liu AYC, Bernards A, Sloof P, van der Bijl MMW, Schinkel AH, Menke HM, Borst P, Veenman GH, Tromp MC, van Boom JH. Activation of the genes for variant surface glycoproteins 117 and 118 in Trypanosoma brucei. J Mol Biol 1983;167:57-75.
29. [29] van der Ploeg L, Smith CL, Polvere RI, Gottesdiener KM. Improved separation of chromosome-sized DNA from Trypanosoma brucei, stock 427-60. Nucleic Acids Res 1989;17:3217-27.
30. [30] Gottesdiener K, Garcia-Anoveros J, Lee MGS, van der Ploeg LHT. Chromosome organization of the protozoan Trypanosoma brucei. Mol Cell Biol 1990;10:6079-83.
31. [31] Hirumi H, Hirumi K. Continuous cultivation of Trypanosoma brucei. blood stream forms in a medium containing a low concentration of serum protein without feeder cell layers. J Parasitol 1989;75:985-9.
32. [32] Rudenko G, McCulloch R, Dirksmulder A, Borst P. Telomere exchange can be an important mechanism of variant surface glycoprotein gene switching in Trypanosoma brucei. Mol Biochem Parasitol 1996;80:65-75.
33. [33] Hug M, Carruthers VB, Hartmann C, Sherman DS, Cross GAM, Clayton C. A possible role for the 3′-untranslated region in developmental regulation in Trypanosoma brucei. Mol Biochem Parasitol 1993;61:87-96.
34. [34] Wirtz E, Clayton C. Inducible gene expression in trypanosomes mediated by a prokaryotic repressor. Science 1995;268:1179-83.
35. [35] Hotz HR, Lorenz P, Fischer R, Krieger S, Clayton C. Role of 3′-untranslated regions in the regulation of hexose transporter mRNAs in Trypanosoma brucei. Mol Biochem Parasitol 1995;75:1-14.
36. [36] van den Hoff MJB, Moorman AFM, Lamers WH. Electroporation in 'intracellular' buffer increases cell survival. Nucleic Acids Res 1992;20:2902.
37. [37] Sambrook J, Fritsch EF, Maniatis T. Molecular Cloning; A Laboratory Manual. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press, 1989.
38. [38] Carruthers VB, van der Ploeg LHT, Cross GAM. DNA-mediated transformation of bloodstream-form Trypanosoma brucei. Nucleic Acids Res 1993;21:2537-8.
39. [39] Horn D, Cross GAM. A developmentally regulated position effect at a telomeric locus in Trypanosoma brucei. Cell 1995;83:555-61.
40. [40] Carruthers VB, Navarro M, Cross GAM. Targeted disruption of expression-site-associated gene-1 in bloodstream-form Trypanosoma brucei. Mol Biochem Parasitol 1996;81:65-79.
41. [41] Horn D, Cross GAM. Analysis of Trypanosoma brucei vsg expression site switching in vitro. Mol Biochem Parasitol 1997;84:189-201.
42. [42] Pays E, Tebabi P, Pays A, Coquelet H, Revelard P, Salmon D, Steinert M. The genes and transcripts of an antigen expression site from T. brucei. Cell 1989;57:835-45.
43. [43] Jefferies D, Tebabi P, Pays E. Transient activity assays of the Trypanosoma brucei variant surface glycoprotein gene promoter: Control of gene expression at the posttranscriptional level. Mol Cell Biol 1991;11:338-43.
44. [44] Urmenyi TP, van der Ploeg LHT. PARP promoter-mediated activation of a VSG expression site promoter in insect form Trypanosoma brucei. Nucleic Acids Res 1995;23:1010-8.
45. [45] Vanhamme L, Berberof M, Le Ray D, Pays E. Stimuli of differentiation regulate RNA elongation in the transcription units for the major stage-specific antigens of Trypanosoma brucei. Nucleic Acids Res 1995;23:1862-9.
46. [46] Moss T, Stefanovsky VY. Promotion and regulation of ribosomal transcription in eukaryotes by RNA polymerase I. Prog Nucleic Acid Res Mol Biol 1995;50:25-66.
47. [47] Brown SD, Huang J, van der Ploeg LHT. The promoter for the procyclic acidic repetitive protein (PARP) genes of Trypanosoma brucei shares features with RNA polymerase I promoters. Mol Cell Biol 1992;12:2644-52.
48. [48] Rudenko G, Blundell PA, Taylor MC, Kieft R, Borst P. VSG gene expression site control in insect form Trypanosoma brucei. EMBO J 1994;13:5470-82.
49. [49] El-Sayed NMA, Alarcon CM, Beck JC, Sheffield VC, Donelson JE. cDNA expressed sequence tags of Trypanosoma brucei rhodesiense. Mol Biochem Parasitol 1995;73:75-90.
50. [50] Hoffmann A, Oelgeschlager T, Roeder RG. Considerations of transcriptional control mechanisms: do TFIID-core promoter complexes recapitulate nucleosome-like functions. Proc Natl Acad Sci USA 1997;94:8928-35.
51. [51] Cross GAM. Release and purification of Trypanosoma brucei variant surface glycoprotein. J Cell Biochem 1984;24:79-90.