Recent advances in microbial polyhydroxyalkanoates

Process Biochemistry

Volumn 40, Issue 2, 2005, Pages 607-619

  Khanna, Shilpi ^a   Srivastava, Ashok K ^a  

^a INDIAN INSTITUTE OF TECHNOLOGY DELHI   (India)

Author keywords

Biodegradable; Fermentation; Polyhydroxyalkanoates; Polyhydroxybutyrate; Recombinant E. coli; Transgenic plants

Indexed keywords

BACTERIA; CYTOLOGY; INDUSTRIAL APPLICATIONS; MONOMERS; POLYMERS;

BIODEGRADABLE POLYMERS; INTRACELLULAR CARBON; TRANSGENIC PLANTS;

POLYESTERS;

ALCALIGENES; BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; PSEUDOMONAS;

EID: 7444226964     PISSN: 13595113     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.procbio.2004.01.053     Document Type: Article

References (120)

1. Brandl H., Gross R.A., Lenz R.W., Fuller R.C. Plastics from bacteria and for bacteria: poly(β-hydroxyalkanoates) as natural, biocompatible, and biodegradable polyesters. Adv. Biochem. Eng. Biotechnol. 41:1990;77-93
2. Byrom D. Polymer synthesis by microorganisms: technology and economics. Trends Biotechnol. 5:1987;246-250
3. Steinbuchel A. Polyhydroxyalkanoic acids. In: Byrom D, editor. Biomaterials: novel materials from biological sources. New York: Stockton; 1991. p. 124-213.
4. Bonthrone K.M., Clauss J., Horowitz D.M., Hunter B.K., Sanders J.K.M. The biological and physical chemistry of polyhydroxyalkanoates as seen by NMR spectroscopy. FEMS Microbiol. Rev. 10:1992;269-278
5. Mergaert J., Anderson C., Wouters A., Swings J., Kersters K. Biodegradation of polyhydroxyalkanoates. FEMS Microbiol. Rev. 10:1992;317-322
6. Byrom D. Polyhydroxyalkanoates. In: Mobley DP, editor. Plastics from microbes: microbial synthesis of polymers and polymer precursors. Munich: Hanser; 1994. p. 5-33.
7. Dawes E.A., Senior P.J. The role and regulation of energy reserve polymers in microorganisms. Adv. Microbiol. Physiol. 10:1973;135-266
8. Burdon K.L. Fatty material in bacteria and fungi revealed by staining dried, fixed slide preparations. J. Bacteriol. 52:1946;665-678
9. Williamson D.H., Wilkinson J.F. The isolation and estimation of the poly-β-hydroxybutyrate inclusions of Bacillus species. J. Gen. Microbiol. 19:1958;198-209
10. Kallio R.E., Harrington A.A. Sudanophillic granules and lipid of Pseudomonas methanica. J. Bacteriol. 80:1960;321-324
11. Ostle A.G., Holt J.G. Nile Blue A as a fluorescent stain for poly-β-hydroxybutyrate. Appl. Environ. Microbiol. 44:1982;238-241
12. Lemoigne M. Products of dehydration and of polymerization of β-hydroxybutyric acid. Bull. Soc. Chem. Biol. 8:1926;770-782
13. Anderson A.J., Dawes E.A. Occurrence, metabolism, metabolic role, and industrial uses of bacterial polyhydroxyalkanoates. Microbiol. Rev. 54:1990;450-472
14. Lagaveen R.G., Huisman G.W., Preusting H., Ketelaar P., Eggink G., Witholt B. Formation of polyesters by Pseudomonas oleovorans; effect of substrates on formation and composition of poly-(R)-3-hydroxyalkanoates and poly-(R)-3-hydroxyalkenoates. Appl. Environ. Microbiol. 54:1988;2924-2932
15. Brandl H., Gross R.A., Lenz R.W., Fuller R.C. Pseudomonas oleovorans as a source of poly(β-hydroxyalkanoates) for potential applications as biodegradable polyesters. Appl. Environ. Microbiol. 54:1988;1977-1982
16. De Smet M.J., Eggink G., Witholt B., Kingma J., Wynberg H. Characterization of intracellular inclusions formed by Pseudomonas oleovorans during growth on octane. J. Bacteriol. 154:1983;870-878
17. Kim B.S., Lee S.C., Lee S.Y., Chang H.N., Chang Y.K., Woo S.I. Production of polyhydroxybutyrate by fed batch with glucose concentration control in Ralstonia eutropha. Biotechnol. Bioeng. 43:1994;892-898
18. Yamane T. Yield of poly-D(-)-3-hydroxybutyrate from various carbon sources; a theoretical study. Biotechnol. Bioeng. 41:1993;167-170
19. Linko S., Vaheri H.J.S. Production poly-β-hydroxybutyrate on lactic acid by Alcaligenes eutrophus H16 in a 3-l bioreactor. Enzyme Microb. Technol. 15:1993;401-406
20. Tsuje T., Tanaka K., Shimoda M., Ishizaki A. Optimization of L-lactic acid feeding for the production of poly-β-hydroxybutyric acid by Alcaligenes eutrophus in fed batch culture. J. Biosci. Bioeng. 88:1999;404-409
21. Wang J., Yu J. Kinetic analysis on formation of poly(3-hydroxybutyrate) from acetic acid by Ralstonia eutropha under chemically defined conditions. J. Ind. Microbiol. Biotechnol. 26:2001;121-126
22. Du G., Chen J., Yu J., Lun S. Continuous production of poly-3-hydroxybutyrate by Ralstonia eutropha in a two-stage culture system. J. Biotechnol. 88:2001;59-65
23. Fukui T., Doi Y. Efficient production of polyhydroxyalkanoates from plant oils by Alcaligenes eutrophus and its recombinant strain. Appl. Microbiol. Biotechnol. 49:1998;333-336
24. Eggink G., van der Wal H., Huijberts G.N.M., de Waard P. Oleic acid as a substrate for poly-3-hydroxybutyrate formation in Alcaligenes eutrophus and Pseudomonas putida. Ind. Crop Prod. 1:1993;157-163
25. Bormann E., Roth M. The production of polyhydroxybutyrate by Methylobacterium rhodesianum and Ralstonia eutropha in media containing glycerol and casein hydrolysates. Biotechnol. Lett. 21:1999;1059-1063
26. Doi Y., Segawa A., Kunioka M. Biosynthesis and characterization of poly(3-hydroxybutyrate-co-4-hydroxybutyrate) in Alcaligenes eutrophus. Int. J. Biol. Macromolecules. 12:1990;106-111
27. Du G., Si Y., Yu J. Inhibitory effect of medium-chain-length fatty acids on synthesis of polyhydroxyalkanoates from volatile fatty acids by Ralstonia eutropha. Biotechnol. Lett. 23:2001;1613-1617
28. Akiyama M., Taima Y., Doi Y. Production of poly(3-hydroxyalkanoates) by a bacterium of the genus Alcaligenes utilizing long-chain fatty acids. Appl. Microbiol. Biotechnol. 37:1992;698-701
29. Fukui T., Kichise T., Yoshida Y., Doi Y. Biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxyvalerate-co-3-hydroxyheptanoate) terpolymers by recombinant Alcaligenes eutrophus. Biotechnol. Lett. 19:1997;1093-1097
30. Akiyama M., Tsuge T., Doi Y. Environmental life cycle comparison of polyhydroxyalkanoates produced from renewable carbon resources by bacterial fermentation. Polym. Degrad. Stab. 80:2003;183-194
31. Jung Y.M., Lee Y.H. Utilization of oxidative pressure for enhanced production of poly-beta-hydroxybutyrate and poly(3-hydroxybutyrate-3- hydroxyvalerate) in Ralstonia eutropha. J. Ferment. Bioeng. 90:2000;266-270
32. Fuechtenbusch B., Wullbrandt D., Steinbuechel A. Production of polyhydroxyalkanoic acids by Ralstonia eutropha and Pseudomonas oleovorans from oil remaining from biotechnological rhamnose production. Appl. Microbiol. Biotechnol. 53:2000;167-172
33. Tsuje T., Tanaka K., Ishizaki A. Development of novel method for feeding a mixture of L-lactic acid and acetic acid in fed-batch culture of Ralstonia eutropha for poly-D-3-hydroxybutyrate production. J. Ferment. Bioeng. 91:2001;545-550
34. Tohyama M., Takagi S., Shimizu K. Effect of controlling lactate concentration and periodic change in DO concentration on fermentation characteristics of a mixed culture of Lactobacillus delbrueckii and Ralstonia eutropha for PHB production. J. Ferment. Bioeng. 89:2000;323-328
35. Tohyama M., Shimizu K. Control of a mixed culture of Lactobacillus delbrueckii and Ralstonia eutropha for the production of PHB from glucose via lactate. Biochem. Eng. J. 4:1999;45-53
36. Katoh T., Yuguchi D., Yoshii H., Shi H., Shimizu K. Dynamics and modeling on fermentative production of poly(β-hydroxybutyric acid) from sugars via lactate by a mixed culture of Lactobacillus delbrueckii and Alcaligenes eutrophus. J. Biotechnol. 67:1999;113-134
37. Tohyama M., Patarinska T., Qiang Z., Shimizu K. Modeling of the mixed culture and periodic control for PHB production. Biochem. Eng. J. 10:2002;157-173
38. Kobayashi G., Tanaka K., Itoh H., Tsuge T., Sonomoto K., Ishikazi A. Fermentative production of P(HB-co-3HV) from propionic acid by Alcaligenes eutrophus in fed-batch culture with pH-stat continuous substrate feeding method. Biotechnol. Lett. 22:2000;1067-1069
39. Song C., Zhao L., Ono S., Shimasaki C., Inoue M. Production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) from cottonseed oil and valeric acid in batch culture of Ralstonia sp. strain JC-64. Appl. Biochem. Biotechnol. 94:2001;169-178
40. Wang F., Lee S.Y. Poly(3-hydroxybutyrate) production with high productivity and high polymer content by a fed-batch culture of Alcaligenes latus under nitrogen limitation. Appl. Environ. Microbiol. 63:1997;3703-3706
41. Grothe E., Moo-Young M., Chisti Y. Fermentation optimization for the production of poly(β-hydroxybutyric acid) microbial thermoplastic. Enzyme Microb. Technol. 25:1999;132-141
42. Grothe E., Chisti Y. Poly(β-hydroxybutyric acid) thermoplastic production by Alcaligenes latus: behavior of fed-batch cultures. Bioprocess Eng. 22:2000;441-449
43. Bourque D., Ouellette B., Andre G., Groleau D. Production of poly-beta-hydroxybutyrate from methanol: characterization of a new isolate of Methylobacterium extorquens. Appl. Microbiol. Biotechnol. 37:1992;7-12
44. Suzuki T., Yamane T., Shimizu S. Mass production of poly-beta- hydroxybutyric acid by fed-batch culture with controlled carbon/nitrogen feeding. Appl. Microbiol. Biotechnol. 24:1986;370-374
45. Preusting H., Hazenberg W., Witholt B. Continuous production of poly(3-hydroxyalkanoates) by Pseudomonas oleovorans in a high cell density, two-liquid-phase chemostat. Enzyme Microb. Technol. 15:1993;311-316
46. Ashby R.D., Solaiman D.K.Y., Foglia T.A. The synthesis of short- and medium-chain-length poly(hydroxyalkanoates) mixtures from glucose- or alkanoic acid-grown Pseudomonas oleovorans. J. Ind. Microbiol. Biotechnol. 28:2002;147-153
47. Fuechtenbusch B., Steinbuechel A. Biosynthesis of polyhydroxyalkanoates from low rank coal liquefaction products by Pseudomonas oleovorans. Appl. Microbiol. Biotechnol. 52:1999;91-95
48. Guo-Qiang C., Jun X., Qiong W., Zengming Z., Kwok-Ping H. Synthesis of copolyesters consisting of medium-chain-length β-hydroxyalkanoates by Pseudomonas stutzeri 1317. React. Funct. Polym. 48:2001;107-112
49. Dennis D., McCoy M., Stangl A., Valentin H.E., Wu Z. Formation of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) by PHA synthase from Ralstonia eutropha. J. Biotechnol. 64:1998;177-186
50. Fidler S., Dennis D. Polyhydroxyalkanoate production in recombinant Escherichia coli. FEMS Microbiol. Rev. 103:1992;231-236
51. Hahn S.K., Chang Y.K., Lee S.Y. Recovery and characterization of poly(3-hydroxybutyric acid) synthesized in Alcaligenes eutrophus and recombinant Escherichia coli. Appl. Environ. Microbiol. 61:1995;34-39
52. Kim B.S., Lee S.Y., Chang H.N. Production of poly-beta-hydroxybutyrate by fed-batch culture of recombinant Escherichia coli. Biotechnol. Lett. 14:1992;811-816
53. Liu F., Li W., Ridgway D., Gu T. Production of poly-beta-hydroxybutyrate on molasses by recombinant Escherichia coli. Biotechnol. Lett. 20:1998;345-348
54. Song S., Hein S., Steinbuechel A. Production of poly(4-hydroxybutyric acid) by fed-batch cultures of recombinant strains of Escherichia coli. Biotechnol. Lett. 21:1999;193-197
55. Park S.J., Ahn W.S., Green P.R., Lee S.Y. Biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxyvalerate-co-3-hydroxyhexanoate) by metabolically engineered Escherichia coli. Biotechnol. Bioeng. 74:2001;81-86
56. Valentin H.E., Dennis D. Production of poly(3-hydroxybutyrate-co-4- hydroxybutyrate) in recombinant Escherichia coli grown on glucose. J. Biotechnol. 58:1997;33-38
57. Ramachander T.V.N., Rohini D., Belhekar A., Rawal S.K. Synthesis of PHB by recombinant E. coli harboring an approximately 5 kb genomic DNA fragment from Streptomyces aureofaciens NRRL 2209. Int. J. Biol. Macromolecules. 31:2002;63-69
58. Ahn W.S., Park S.J., Lee S.Y. Production of poly(3-hydroxybutyrate) by fed-batch culture of recombinant Escherichia coli with a highly concentrated whey solution. Appl. Environ. Microbiol. 66:2000;3624-3627
59. Yu H., Shi Y., Zhang Y., Yang S., Shen Z. Effect of Vitreoscilla hemoglobin biosynthesis in Escherichia coli on production of poly(β-hydroxybutyrate) and fermentative parameters. FEMS Microbiol. Lett. 214:2002;223-227
60. van Niel W.J., Robertson L.A., Kuenen J.G. Rapid short-term poly-β-hydroxybutyrate production by Thiosphaera pantotropha in the presence of excess acetate. Enzyme Microb. Technol. 17:1995;977-982
61. Qi Q., Rehm B.H.A. Polyhydroxybutyrate biosynthesis in Caulobacter crescentus: molecular characterization of the polyhydroxybutyrate-synthase. Microbiology. 147:2001;3353-3358
62. Pettinari M.J., Vazquez G.J., Silberschmidt D., Rehm B., Steinbuechel A., Mendez B.S. Poly(3-hydroxybutyrate) synthesis genes in Azotobacter sp. strain FA8. Appl. Environ. Microbiol. 67:2001;5331-5334
63. Miyake M., Erata M., Asada Y. A thermophillic cyanobacterium, Synechococcus sp. MA19, capable of accumulating poly-β-hydroxybutyrate. J. Ferment. Bioeng. 82:1996;512-514
64. Nishioka M., Nakai K., Miyake M., Asada Y., Taya M. Production of poly-beta-hydroxybutyrate by thermophillic cyanobacterium, Synechococcus sp. MA19, under phosphate-limited conditions. Biotechnol. Lett. 23:2001;1095-1099
65. Wu G.F., Wu Q.Y., Shen Z.Y. Accumulation of poly-beta-hydroxybutyrate in cyanobacterium Synechocystis sp. PCC6803. Bioresour. Technol. 76:2001;85-90
66. Carlozzi P., Sacchi A. Biomass production and studies on Rhodopseudomonas palustris grown in an outdoor, temperature controlled, underwater tubular photobioreactor. J. Biotechnol. 88:2001;239-249
67. Labuzek S., Radecka I. Biosynthesis of PHB tercopolymer by Bacillus cereus UW85. J. Appl. Microbiol. 90:2001;353-357
68. Huang J.X., Wang R., Qin H.M. Fermentation conditions for producing poly-beta-hydroxybutyric acid by strain Z5-GII. Ind. Microbiol. 29:1999;20-24
69. Ostafin M., Haber J., Doronina N.V., Sokolov A.P., Trotsenko Y.A. Methylobacterium extroquens strain P14, a new methylotrophic bacteria producing poly-beta-hydroxybutyrate (PHB). Acta Microbiol. Pol. 48:1999;39-51
70. Borah B., Thakur P.S., Nigam J.N. The influence of nutritional and environmental conditions on the accumulation of poly-beta-hydroxybutyrate in Bacillus mycoides RLJ B-017. J. Appl. Microbiol. 92:2002;776-783
71. Daniel K., Saul B., Edouard J., Yaacov O. Identification and isolation of genes involved in poly(beta-hydroxybutyrate) biosynthesis in Azospirillum brasilense and characterization of a phbC mutant. Appl. Environ. Microbiol. 68:2002;2943-2949
72. Falvo A., Levantesi C., Rossetti S., Seviour R.J., Tandoi V. Synthesis of intracellular storage polymers by Amaricoccus kaplicensis, a tetrad forming bacterium present in activated sludge. J. Appl. Microbiol. 91:2001;299-305
73. Poirier Y., Nawrath C., Somerville C. Production of polyhydroxyalkanoates, a family of biodegradable plastics and elastomers, in bacteria and plants. Biotechnology. 13:1995;142-150
74. Ramsay J.A., Hassan M.C.A., Ramsay B.A. Hemicellulose as a potential substrate for production of poly(beta-hydroxyalkanoates). Can. J. Microbiol. 41:1995;262-266
75. Hu W.F., Chua H., Yu P.H.F. Synthesis of poly(3-hydroxybutyrate-co-3- hydroxyvalerate) from activated sludge. Biotechnol. Lett. 19:1997;695-698
76. Bormann E.J., Leissner M., Roth M., Beer B., Metzner K. Production of polyhydroxybutyrate by Ralstonia eutropha from protein hydrolyzates. Appl. Microbiol. Biotechnol. 50:1998;604-607
77. Omar S., Rayes A., Eqaab A., Viss I., Steinbuechel A. Optimization of cell growth and poly(3-hydroxybutyrate) accumulation on date syrup by a Bacillus megaterium strain. Biotechnol. Lett. 23:2001;1119-1123
78. Poirier Y., Dennis D.E., Klomparens K., Somerville C. Polyhydroxybutyrate, a biodegradable thermoplastic, produce in transgenic plants. Science. 256:1992;520-523
79. John M.E., Keller G. Metabolic pathway engineering in cotton: biosynthesis of polyhydroxybutyrate in fiber cells. Proc. Natl. Acad. Sci. U.S.A. 93:1996;12768-12773
80. Nakashita H., Arai Y., Yoshioka K., Fukui T., Doi Y., Usami R., et al. Production of biodegradable polyester by a transgenic tobacco. Biosci. Biotechnol. Biochem. 63:1999;870-874
81. Nawrath C., Poirier Y., Somerville C. Targeting of the polyhydroxybutyrate biosynthetic pathway to the plastids of Arabidopsis thaliana results in high levels of polymer accumulation. Proc. Natl. Acad. Sci. U.S.A. 91:1994;12760-12764
82. Houmiel K.L., Slater S., Broyles D., Casagrande L., Colburn S., Gonzalez K., et al. Poly(β-hydroxybutyrate) production in oilseed leucoplasts of Brassica napus. Planta. 209:1999;547-550
83. Rehm B.H.A., Kruger N., Steinbuchel A. The new metabolic link between fatty acid de novo synthesis and polyhydroxyalkanoic acid synthesis. J. Biol. Chem. 273:1998;044-51
84. Van der Leij F.R., Witholt B. Strategies for the sustainable production of new biodegradable polyesters in plants: a review. Can. J. Microbiol. 41:1995;222-238
85. Sudesh K., Abe H., Doi Y. Synthesis, structure and properties of polyhydroxyalkanoates: biological polyesters. Prog. Polym. Sci. 25:2000;1503-1555
86. Barnard G.N., Sanders J.K. The poly-β-hydroxybutyrate granule in vivo. A new insight based on NMR spectroscopy of whole cells. J. Biol. Chem. 264:1989;3286-3291
87. Preusting H., Nijenhuis A., Witholt B. Physical characteristics of poly(3-hydroxyalkanoates) and poly(3-hydroxyalkenoates) produced by Pseudomonas oleovorans grown on aliphatic hydrocarbons. Macromolecules. 23:1990;4220-4224
88. Macrae R.M., Wilkinson J.R. Poly-β-hydroxybutyrate metabolism in washed suspensions of Bacillus cereus and Bacillus megaterium. J. Gen. Microbiol. 19:1958;210-222
89. De Koning G.J.M., Lemastra P.J., Hill D.J.T., Carswell T.G., O'Donell J.H. Ageing phenomena in bacterial poly[(R)-3-hydroxybutyrate]. Polymer. 33:1992;3295-3297
90. De Koning G.J.M., Lemstra P.J. Crystallization phenomena in bacterial poly[(R)-3-hydroxybutyrate]. 2. Embrittlement and rejuvenation. Polymer. 34:1993;4089-4094
91. De Koning G.J.M., Scheeren A.H.C., Lemstra P.J., Peeters M., Reynaers H. Crystallization phenomena in bacterial poly[(R)-3-hydroxybutyrate]. 3 Toughening via texture changes. Polymer. 35:1994;4598-4605
92. Orts W.J., Marchessault R.H., Bluhm T.L. Thermodynamics of the melting point depression in poly(β-hydroxybutyrate-co-β-hydroxyvalerate) copolymers. Macromolecules. 24:1991;6435-6438
93. Oeding V., Schlegel H.G. Beta-ketothiolase from Hydrogenomonas eutropha HI6 and its significance in the regulation of poly-beta-hydroxybutyrate metabolism. Biochem. J. 134:1973;239-248
94. Senior P.J., Dawes E.A. The regulation of poly-beta-hydroxybutyrate metabolism in Azotobacter beirjerinckii. Biochem. J. 134:1973;225-238
95. Law J.H., Slepecky R.A. Assay of poly-β-hydroxybutyric acid. J. Bacteriol. 82:1969;52-55
96. Jüttner R.R., Lafferty R.M., Knackmuss H.J. A simple method for the determination of poly-β-hydroxybutyric acid in microbial biomass. Eur. J. Appl. Microbiol. 1:1975;233-237
97. Lee S.Y. Plastic bacteria? Progress and prospects for polyhydroxyalkanoates production in bacteria. Trends Biotechnol. 14:1996;431-438
98. Lafferty RM, Korsatko B, Korsatko W. Microbial production of poly-β-hydroxybutyric acid. In: Rehm HJ, Reed G, editors. Biotechnology. Special microbial processes. 6B ed. Weinheim: UCH; 1988. p. 136-76.
99. Braunegg G., Sonnleitner B., Lafferty R.M. A rapid gas chromatographic method for the determination of poly-n-hydroxybutyric acid in microbial diversity. Eur. J. Appl. Microbiol. Biotechnol. 6:1978;29-37
100. Riis V., Mai W. Gas chromatographic determination of poly-n- hydroxybutyric acid in microbial biomass after hydrochloric propanolysis. J. Chromatogr. 445:1988;285-289
101. Karr D.B., Waters J.K., Emerich D.W. Analysis of poly-β- hydroxybutyrate in Rhizobium japonicum bacteroids by ion-exclusion high-pressure liquid chromatography and UV detection. Appl. Environ. Microbiol. 46:1983;1339-1344
102. Hesselmann R.P.X., Fleischmann T., Hany R., Zehnder A.J.B. Determination of polyhydroxyalkanoates in activated sludge by ion chromatographic and enzymatic methods. J. Microbiol. Methods. 35:1999;111-119
103. Degelau A., Scheper T., Bailey J.E., Guske C. Fluorometric measurement of poly-β-hydroxybutyrate in Alcaligenes eutrophus by flow cytometry and spectrofluorometry. Appl. Microbiol. Biotechnol. 42:1995;653-657
104. Gorenflo V., Steinbuchel A., Marose S., Riesberg M., Scheper T. Quantification of bacterial polyhydroxyalkanoic acids by Nile Red staining. Appl. Microbiol. Biotechnol. 51:1999;765-772
105. Jendrossek D., Frisse A., Behrends A., Andermann M., Kratzin H.D., Stanislawski T., et al. Biochemical and molecular characterization of the Pseudomonas lemoignei polyhydroxyalkanoate depolymerase system. J. Bacteriol. 177:1995;596-607
106. Kasuya K., Inoue Y., Doi Y. Adsorption kinetics of bacterial PHB depolymerase on the surface of polyhydroxyalkanoate films. Int. J. Biol. Macromolecules. 19:1996;35-40
107. Kasuya K., Inoue Y., Yamada K., Doi Y. Kinetics of surface hydrolysis of poly((R)-3-hydroxybutyrate) film by PHB depolymerase from Alcaligenes faecalis T1. Polym. Degrad. Stab. 48:1995;167-174
108. Shirakura Y., Fukui T., Saito T., Okamoto Y., Narikawa T., Koide K., et al. Degradation of poly(3-hydroxybutyrate) by poly(3-hydroxybutyrate) depolymerase from Alcaligenes faecalis T1. Biochim. Biophys. Acta. 880:1986;46-53
109. Lee S.Y. Bacterial polyhydroxyalkanoates. Biotechnol. Bioeng. 49:1995;1-14
110. Doi Y., Segawa A., Kunioka M. Biosynthesis and characterization of poly(3-hydroxybutyrate-co-4-hydroxybutyrate) in Alcaligenes eutrophus. Int. J. Biol. Macromolecules. 12:1990;101-111
111. Kunioka M., Kawaguchi Y., Doi Y. Production of biodegradable copolyesters of 3-hydroxybutyrate and 4-hydroxybutyrate by Alcaligenes eutrophus. Appl. Microbiol. Biotechnol. 30:1989;569-573
112. Saito Y., Doi Y. Microbial synthesis and properties of poly(3-hydroxybutyrate-co-4-hydroxybutyrate) in Comamonas acidovorans. Int. J. Biol. Macromolecules. 16:1994;99-104
113. Mergaert J., Webb A., Anderson C., Wouters A., Swings J. Microbial degradation of poly(3-hydroxybutyrate) and poly(3-hydroxybutyrate-co-3- hydroxyvalerate) in soils. Appl. Environ. Microbiol. 59:1993;3233-3238
114. Jendrossek D., Knoke I., Habibian R.B., Steinbuchel A., Schlegel H.G. Degradation of poly(3-hydroxybutyrate), PHB, by bacteria and purification of a novel PHB depolymerase from Comamonas sp. J. Environ. Polym. Degrad. 1:1993;53-63
115. Delafield F.P., Douroroff M., Paileroni N.J., Lusty J., Contropoulos R. Decomposition of poly-β-hydroxybutyrate by pseudomonas. J. Bacteriol. 90:1965;1455-1466
116. Tanio T., Fukui T., Shirakura Y., Saito T., Tomita K., Kaiho T., et al. An extracellular poly(3-hydroxybutyrate) depolymerase from Alcaligenes faecalis. Eur. J. Biochem. 124:1982;71-77
117. Schirmer A., Jendrossek D., Schelgel H.G. Degradation of poly(3-hydroxyoctanoic acid) [P(3HO)] by bacteria: purification and properties of a P(3HO) depolymerase from Pseudomonas fluorescens GK13. Appl. Environ. Microbiol. 59:1993;1220-1227
118. Kasuya K., Doi Y., Yao T. Enzymatic degradation of poly[(R)-3- hydroxybutyrate] by Comamomas testosteroni ATSU of soil bacterium. Polym. Degrad. Stab. 45:1994;379-386
119. Mukai K., Yamada K., Doi Y. Efficient hydrolysis of poly- hydroxyalkanoates by Pseudomonas stutzeri YM1414 isolated from lake water. Polym. Degrad. Stab. 43:1994;319-327
120. Jansen P.H., Harfoot C.G. Ilyobacter delafieldii sp. nov., a metabolically restricted anaerobic bacterium fermenting PHB. Arch. Microbiol. 154:1990;253-259