Polyhydroxybutyrate biosynthesis in Caulobacter crescentus: Molecular characterization of the polyhydroxybutyrate synthase

Microbiology

Volumn 147, Issue 12, 2001, Pages 3353-3358

  Qi, Q ^a   Rehm, B H A ^a  

^a UNIVERSITY OF MÜNSTER   (Germany)

Author keywords

PHA depolymerase; PHA synthase; Polyhydroxyalkanoate

Indexed keywords

3 HYDROXYACYL COENZYME A DEHYDROGENASE; 3 HYDROXYBUTYRIC ACID; ACETYL COENZYME A ACYLTRANSFERASE; BACTERIAL PROTEIN; GLUCOSE; PEPTIDE SYNTHASE; POLY(3 HYDROXYBUTYRIC ACID); POLYESTER; POLYHYDROXYALKANOIC ACID;

ARTICLE; AZORHIZOBIUM CAULINODANS; BIOSYNTHESIS; CAULOBACTER CRESCENTUS; CONTROLLED STUDY; ENZYME SYNTHESIS; GAS CHROMATOGRAPHY; GEL PERMEATION CHROMATOGRAPHY; GENE CONTROL; GENE EXPRESSION; GENE FUNCTION; GENE SEQUENCE; GENETIC CODE; IMMUNOBLOTTING; IN VITRO STUDY; MASS SPECTROMETRY; MOLECULAR CLONING; MOLECULAR DYNAMICS; MOLECULAR WEIGHT; MUTANT; NONHUMAN; NUCLEOTIDE SEQUENCE; OPEN READING FRAME; PLASMID; PRIORITY JOURNAL; PROMOTER REGION; SEQUENCE HOMOLOGY; WAUTERSIA EUTROPHA;

AZORHIZOBIUM CAULINODANS; CAULOBACTER CRESCENTUS; RALSTONIA EUTROPHA;

EID: 0035218357     PISSN: 13500872     EISSN: None     Source Type: Journal    
DOI: 10.1099/00221287-147-12-3353     Document Type: Article

References (37)

1. Pseudomonas oleovorans as a source of poly(β-hydroxyalkanoates) for potential applications as biodegradable polyesters
2. PhaG-mediated synthesis of poly(3-hydroxyalkanoates) consisting of medium-chain-length constituents from nonrelated carbon sources in recombinant Pseudomonas fragi
3. Naturally occurring genetic transfer of hydrogen-oxidizing ability between strains of Alcaligenes eutrophus
4. Expression and characterization of (R)-specific enoyl coenzyme A hydratase involved in polyhydroxyalkanoate biosynthesis by Aeromonas caviae
5. Overexpression and purification of the soluble polyhydroxyalkanoate synthase from Alcaligenes eutrophus-evidence for a required posttranslational modification for catalytic activity
6. Mobilization of poly(3-hydroxybutyrate) in Ralstonia eutropha
7. Accumulation of a poly(hydroxyalkanoate) copolymer containing primarily 3-hydroxyvalerate from simple carbohydrate substrates by Rhodococcus sp. NCIMB 40126
8. The Pseudomonas aeruginosa phaG gene product is involved in the synthesis of polyhydroxyalkanoic acid consisting of medium-chain-length constituents from non-related carbon sources
9. Homologous functional expression of cryptic phaG from Pseudomonas oleovorans establishes the transacylase-mediated polyhydroxy-alkanoate biosynthetic pathway
10. Analysis of 4-phosphopantetheinylation of polyhydroxybutyrate synthase from Ralstonia eutropha: Generation of beta-alanine auxotrophic Tn5 mutants and cloning of the panD gene region
11. Lipases provide a new mechanistic model for polyhydroxybutyrate (PHB) synthases: Characterization of the functional residues in Chromatium vinosum PHB synthase
12. Mechanistic studies on class I polyhydroxybutyrate (PHB) synthase from Ralstonia eutropha: Class I and III synthases share a similar catalytic mechanism
13. 4 new derivatives of the broad-host-range cloning vector pBBR1MCS, carrying different antibiotic-resistance cassettes
14. Cleavage of structural proteins during the assembly of the head of bacteriophage T4
15. Functional expression of the PHA synthase gene phaC1 from Pseudomonas aeruginosa in Escherichia coli results in poly(3-hydroxyalkanoate) synthesis
16. Purification and characterization of the poly(hydroxyalkanoic acid) synthase from Chromatium vinosum and localization of the enzyme at the surface of poly(hydroxyalkanoic acid) granules
17. An improved procedure for direct phenol extraction of single-stranded M13-DNA for sequencing
18. Complete genome sequence of Caulobacter crescentus
19. Poly-β-hydroxybutyrate biosynthesis in Alcaligenes eutrophus H16 - Characterization of the genes encoding β-ketothiolase and acetoacetyl-CoA reductase
20. Poly-β-hydroxybutyrate biosynthesis in Alcaligenes eutrophus H16 - Identification and characterization of the PHB polymerase gene (phbC)
21. The Caulobacters - Ubiquitous unusual bacteria
22. Synthesis of poly(3-hydroxyalkanoates) in Escherichia coli expressing the PHA synthase gene phaC2 from Pseudomonas aeruginosa: Comparison of PhaC1 and PhaC2
23. Metabolic routing towards polyhydroxyalkanoic acid synthesis in recombinant Escherichia coli (fadR): Inhibition of fatty acid beta-oxidation by acrylic acid
24. In vitro synthesis of poly(3-hydroxydecanoate): Purification and enzymatic characterization of type II polyhydroxyalkanoate synthases PhaC1 and PhaC2 from Pseudomonas aeruginosa
25. Biochemical and genetic analysis of PHA synthases and other proteins required for PHA synthesis
26. Heterologous expression of the acyl-acyl carrier protein thioesterase gene from the plant Umbellularia california mediates polyhydroxyalkanoate biosynthesis in recombinant Escherichia coli
27. PHA synthases - The key enzymes of PHA synthesis
28. A new metabolic link between fatty acid de novo synthesis and poly-hydroxyalkanoic acid synthesis - The phaG gene from Pseudomonas putida KT2440 encodes a 3-hydroxyacyl-acyl carrier protein coenzyme A transferase
29. Matrix-assisted in vitro refolding of Pseudomonas aeruginosa class II polyhydroxyalkanoate synthase from inclusion bodies produced in recombinant Escherichia coli
30. Biosynthesis of latex-like polyhydroxyalkanoates
31. The isolation of mutants not accumulating poly-beta-hydroxybutyric acid
32. Cloning of the Alcaligenes eutrophus genes for synthesis of poly-β-hydroxybutyric acid (PHB) and synthesis of PHB in Escherichia coli
33. Molecular analysis of the Alcaligenes eutrophus poly(3-hydroxybutyrate) biosynthetic operon - Identification of the N-terminus of poly(3-hydroxybutyrate) synthase and identification of the promoter
34. A broad host range mobilization system for in vivo gentetic engineering-transposon mutagenesis in Gram-negative bacteria
35. A sensitive, viable-colony staining method using Nile red for direct screening of bacteria that accumulate polyhydroxyalkanoic acids and other lipid storage compounds
36. Cloning and characterization of the Methylobacterium extorquens polyhydroxyalkanoic-acid-synthase structural gene