Determination of polyhydroxyalkanoates in activated sludge by ion chromatographic and enzymatic methods

Journal of Microbiological Methods

Volumn 35, Issue 2, 1999, Pages 111-119

  Hesselmann, Rolf P X ^a   Fleischmann, Thomas ^a   Hany, Roland ^b   Zehnder, Alexander J B ^a  

^a ETH ZURICH   (Switzerland)

^b SWISS FEDERAL LABORATORIES FOR MATERIALS SCIENCE AND TECHNOLOGY   (Switzerland)

Author keywords

Analysis; Enzymatic; Ion chromatographic; PHB; PHV; Polyhydroxyalkanoates

Indexed keywords

POLYHYDROXYALKANOIC ACID;

ACTIVATED SLUDGE; ARTICLE; DEPOLYMERIZATION; DRUG DETERMINATION; ENZYME ASSAY; HYDROLYSIS; ION PAIR CHROMATOGRAPHY; PRIORITY JOURNAL; TECHNIQUE;

1-PROPANOL; CHROMATOGRAPHY, ION EXCHANGE; COLORIMETRY; ENZYMES; HYDROLYSIS; HYDROXYBUTYRATES; POLYESTERS; SEWAGE; VALERATES;

BACTERIA (MICROORGANISMS); PROSPECT HILL VIRUS;

EID: 0033105035     PISSN: 01677012     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0167-7012(98)00107-9     Document Type: Article

References (22)

1. Bergmeyer, H.U., Gawehn, K., Klotzsch, H., Krebs, H.A., Williamson, D.H., 1967. Purification and properties of crystalline 3-hydroxybutyrate dehydrogenase from Rhodopseudomonas spheroides. Biochem. J. 102, 423-431.
2. Braunegg, G., Sonnleitner, B., Lafferty, R.M., 1978. A rapid gas chromatographie method for the determination of poly-β-hydroxybutyric acid in microbial biomass. Eur. J. Appl. MiCrobiol. Biotechnol. 6, 29-37.
3. Comeau, Y., Hall, K.J., Hancock, R.E.W., Oldham, W.K., 1986. Biochemical model for enhanced biological phosphorus removal. Water Res. 20, 1511-1521.
4. Comeau, Y., Hall, K.J., Oldham, W.K., 1988. Determination of poly-β-hydroxybutyrate and poly-β-hydroxyvalerate in activated sludge by gas-liquid chromatography. Appl. Environ. Microbiol. 54, 2325-2327.
5. Findlay, R.H., White, D.C., 1987. A simplified method for bacterial nutritional status based on the simultaneous determination of phospholipid and endogenous storage lipid poly betahydroxyalkanoate. J. Microbiol. Methods 6, 113-120.
6. Jan, S., Roblot, C., Goethals, G., Courtois, J., Courtois, B., Saucedo, J., Seguin, J., Barbotin, J., 1995. Study of parameters affecting poly(3-hydroxybutyrate) quantification by gas chromatography. Anal. Biochem. 225, 258-263.
7. Jüttner, R.-R., Lafferty, R.M., Knackmuss, H.-J., 1975. A simple method for the determination of poly-β-hydroxybutyric acid in microbial biomass. Eur. J. Appl. Microbiol. Biotechnol. 1, 233-237.
8. Karr, D.B., Waters, J.K., Emerich, D.W., 1983. Analysis of poly-β-hydroxybutyrate in Rhizobium japonicum bacteroids by ion-exclusion high pressure liquid chromatography and UV-detection. Appl. Environ. Microbiol. 46, 1339-1344.
9. Kurth, H., 1997. Determination of 3-hydroxybutyric acid by means of IEC. Customer Application Report. Dionex (Switzerland) AG, Olten, Switzerland.
10. Law, J.H., Slepecky, R.A., 1961. Assay of poly-β-hydroxybutyric acid. J. Bacteriol. 82, 33-36.
11. Lemoigne, M., 1926. Produits de déshydration et de polymérization de l'acid ß-oxybutyrique. Bull. Soc. Chim. Biol. 8, 770-782.
12. Ottaway, J.H., 1962. A preparation of D(-)-β-hydroxybutyric acid. Biochem. J. 84, 11-12.
13. Péaud-Lenoël, C., 1949. Préparation et purification de l'acid L-β-hydroxyoxybutyrique et de ses sels. Bull. Soc. Chim. Biol. 31, 1562-1563.
14. Riis, V., Mai, W., 1988. Gas chromatographic determination of poly-beta-hydroxybutyric acid in microbial biomass after hydrochloric acid propanolysis. J. Chromatogr. 445, 285-289.
15. Seebach, D., Züger, M., 1982. Über die Depolymerisierung von Poly-(R)-3-hydroxy-buttersäureester. Helv. Chim. Acta 65, 495-503.
16. Seebach, D., Roggio, S., Zimmermann, J., 1986. Biological-chemical preparation of 3-hydroxycarboxylic acids and their use in EPC-syntheses. In: Bartmann, W. (Ed.), Stereochemistry of Organic and Bioorganic Transformations. VCH, Weinheim, Germany, pp. 85-125.
17. Seebach, D., Beck, A., Breitschuh, R., Job, K., 1993. Direct degradation of the biopolymer poly[(R)-3-hydroxybutyric acid] to (R)-3-hydroxybutanoic acid and its methyl ester. Organ. Synth. 71, 39-47.
18. Sidler, A., 1997. Personal communication. Fluka Chemie AG, Buchs, Switzerland.
19. Smolders, G.J.F., van der Meij, J., van Loosdrecht, M., Heijnen. J.J., 1994. Model of the anaerobic metabolism of the biological phosphorus removal process: Stoichiometry and pH influence. Biotechnol. Bioeng. 43, 461-470.
20. Steinbuchel, A., 1996. PHB and other polyhydroxyalkanoic acids. In: Roehr, M. (Ed.), Products of Primary Metabolism. VCH, Weinheim, Germany, pp. 403-463.
21. Budavari, S. (Ed.), 1989. The Merck Index, 11th Edition. Merck, Rahway, New York, p. 862.
22. Williamson, D.H., Wilkinson, J.F., 1958. The isolation and estimation of poly-β-hydroxybutyrate inclusions of Bacillus species. J. Gen. Microbiol. 19, 198-209.