Facilitation of polymerase chain reaction with thermostable inorganic pyrophosphatase from hyperthermophilic archaeon Pyrococcus horikoshii

Applied Microbiology and Biotechnology

Volumn 85, Issue 3, 2010, Pages 807-812

  Park, Sun Young ^a   Lee, Bokhui ^a   Park, Kwang Su ^a   Chong, Youhoon ^a   Yoon, Moon Young ^b   Jeon, Sung Jong ^c   Kim, Dong Eun ^a  

^a Konkuk University   (South Korea)

^b Hanyang University   (South Korea)

^c Dong Eui University   (South Korea)

Author keywords

Hyperthermophilic archaeon; Inorganic pyrophosphatase; Polymerase chain reaction; Thermostability

Indexed keywords

CATALYTIC ACTIVITY; CHEMICAL EQUILIBRIUMS; COLORIMETRIC METHODS; DNA POLYMERIZATION; HIGH TEMPERATURE; HYPERTHERMOPHILIC ARCHAEON; INORGANIC PHOSPHATES; INORGANIC PYROPHOSPHATASES; NUMBER OF CYCLES; PCR PRODUCTS; PYROCOCCUS HORIKOSHII; PYROPHOSPHATASE ACTIVITIES;

DNA; ESCHERICHIA COLI; GENES; PHASE EQUILIBRIA; POLYMERIZATION; REDUCING AGENTS; SYNTHESIS (CHEMICAL);

POLYMERASE CHAIN REACTION;

DNA; INORGANIC PYROPHOSPHATASE; PHOSPHATE; RECOMBINANT PROTEIN;

DNA; ENZYME ACTIVITY; GENE EXPRESSION; MICROBIAL ACTIVITY; MOLYBDATE GROUP; POLYMERASE CHAIN REACTION; POLYMERIZATION; PROKARYOTE; REACTION KINETICS; THERMODYNAMICS;

ARTICLE; CATALYSIS; COLORIMETRY; CONTROLLED STUDY; DNA SYNTHESIS; HYPERTHERMOPHILIC ARCHAEON; NONHUMAN; POLYMERASE CHAIN REACTION; POLYMERIZATION; PYROCOCCUS HORIKOSHII; QUANTITATIVE ANALYSIS; THERMOSTABILITY;

ARCHAEAL PROTEINS; CLONING, MOLECULAR; DNA-DIRECTED DNA POLYMERASE; ENZYME STABILITY; ESCHERICHIA COLI; GENE EXPRESSION; HOT TEMPERATURE; INORGANIC PYROPHOSPHATASE; POLYMERASE CHAIN REACTION; PYROCOCCUS HORIKOSHII;

ARCHAEA; ESCHERICHIA COLI; PYROCOCCUS HORIKOSHII;

EID: 74149094746     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00253-009-2314-z     Document Type: Article

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