Characterization of a dUTPase from the hyperthermophilic archaeon Thermococcus onnurineus NA1 and its application in polymerase chain reaction amplification

Marine Biotechnology

Volumn 9, Issue 4, 2007, Pages 450-458

  Cho, Y ^a   Lee, H S ^a   Kim, Y J ^a   Kang, S G ^a   Kim, S J ^a   Lee, J H ^a  

^a Korea Research Institute of Ships and Ocean Engineering   (South Korea)

Author keywords

DNA polymerase; dUTPase; PCR amplification; Thermococcus onnurineus NA1

Indexed keywords

DNA POLYMERASE; DUTPASE; PCR AMPLIFICATION; THERMOCOCCUS ONNURINEUS NA1;

CLONING; CONCENTRATION (PROCESS); ESCHERICHIA COLI; GEL PERMEATION CHROMATOGRAPHY; GENE ENCODING;

ENZYMES;

DUTP PYROPHOSPHATASE; INORGANIC PYROPHOSPHATASE; PRIMER DNA; RECOMBINANT PROTEIN; UNCLASSIFIED DRUG;

BACTERIUM; CLONE; CONCENTRATION (COMPOSITION); ENZYME ACTIVITY; GENETIC ANALYSIS; POLYMERASE CHAIN REACTION; PROKARYOTE; THERMOPHILY;

AMINO ACID SEQUENCE; ARTICLE; BIOSYNTHESIS; CHEMISTRY; ENZYMOLOGY; ESCHERICHIA COLI; GENE EXPRESSION; GENETICS; HUMAN; METABOLISM; METHODOLOGY; MOLECULAR CLONING; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; SEQUENCE ALIGNMENT; TEMPERATURE; THERMOCOCCUS;

AMINO ACID SEQUENCE; BASE SEQUENCE; CLONING, MOLECULAR; DNA PRIMERS; ESCHERICHIA COLI; GENE EXPRESSION; HUMANS; MOLECULAR SEQUENCE DATA; POLYMERASE CHAIN REACTION; PYROPHOSPHATASES; RECOMBINANT PROTEINS; SEQUENCE ALIGNMENT; TEMPERATURE; THERMOCOCCUS;

ARCHAEA; BOVINAE; ESCHERICHIA COLI; PYROCOCCUS FURIOSUS; THERMOCOCCUS;

EID: 34848827123     PISSN: 14362228     EISSN: None     Source Type: Journal    
DOI: 10.1007/s10126-007-9002-8     Document Type: Article

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