Characterization of a dITPase from the hyperthermophilic archaeon Thermococcus onnurineus NA1 and its application in PCR amplification

Applied Microbiology and Biotechnology

Volumn 79, Issue 4, 2008, Pages 571-578

  Kim, Yun Jae ^a,b   Ryu, Yong Gu ^a   Lee, Hyun Sook ^a   Cho, Yona ^a   Kwon, Suk Tae ^b   Lee, Jung Hyun ^a   Kang, Sung Gyun ^a  

^a Korea Research Institute of Ships and Ocean Engineering   (South Korea)

^b Sungkyunkwan University   (South Korea)

Author keywords

dITP generation; dITPase; Family B type DNA polymerase; Hypoxanthine; Thermococcus

Indexed keywords

DNA; REACTION KINETICS;

DITP GENERATION; FAMILY B-TYPE DNA POLYMERASE; HYPOXANTHINE; THERMOCOCCUS;

ENZYME ACTIVITY;

DEOXYINOSINE PYROPHOSPHATASE; DEOXYINOSINE TRIPHOSPHATE; DNA DIRECTED DNA POLYMERASE BETA; INOSINE TRIPHOSPHATE;

BACTERIUM; DNA; GENE; GENOMICS; PHOSPHATE; POLYMERASE CHAIN REACTION; PROTEIN;

ARTICLE; COCCUS (BACTERIUM); ENZYME ANALYSIS; ESCHERICHIA COLI; GENE AMPLIFICATION; GENE EXPRESSION; GENE FUNCTION; GENOMICS; HYDROLYSIS; HYPERTHERMOPHILIC ARCHAEON; METHANOCALDOCOCCUS JANNASCHII; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; OPEN READING FRAME; POLYMERASE CHAIN REACTION; PROTEIN PURIFICATION; PYROCOCCUS FURIOSUS; THERMOCOCCUS ONNURINEUS;

AMINO ACID SEQUENCE; ARCHAEAL PROTEINS; CLONING, MOLECULAR; DEOXYRIBONUCLEOTIDES; DNA-DIRECTED DNA POLYMERASE; INOSINE TRIPHOSPHATE; KINETICS; MOLECULAR SEQUENCE DATA; POLYMERASE CHAIN REACTION; PYROPHOSPHATASES; SEQUENCE ALIGNMENT; THERMOCOCCUS;

ARCHAEA; ESCHERICHIA COLI; METHANOCALDOCOCCUS JANNASCHII; PYROCOCCUS FURIOSUS; THERMOCOCCUS;

EID: 44649096316     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00253-008-1467-5     Document Type: Article

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