Thermo Sequenase(TM) DNA polymerase and T. acidophilum pyrophosphatase: New thermostable enzymes for DNA sequencing

BioTechniques

Volumn 22, Issue 4, 1997, Pages 758-765

  Vander Horn, P B ^a   Davis, M C ^a   Cunniff, J J ^a   Ruan, C ^a   McArdle, B F ^a   Samols, S B ^a   Szasz, J ^a   Hu, G ^a   Hujer, K M ^a   Domke, S T ^a   Brummet, S R ^a   Moffett, R B ^a   Fuller, C W ^a,b  

^a Amersham Biosciences   (United States)

^b Amersham Life Science   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DNA POLYMERASE; INORGANIC PYROPHOSPHATASE; 2',3' DIDEOXYNUCLEOSIDE DERIVATIVE; BACTERIOPHAGE T7 INDUCED DNA POLYMERASE; DNA; DNA DIRECTED DNA POLYMERASE; PYROPHOSPHATE; TAQ POLYMERASE;

ACCURACY; ALLELE; ARTICLE; DNA SEQUENCE; ENZYME STABILITY; GENE AMPLIFICATION; GENETIC ENGINEERING; MOLECULAR CLONING; POLYMERASE CHAIN REACTION; THERMOPLASMA; THERMOSTABILITY; CHEMISTRY; ENZYMOLOGY; GENETICS; HETEROZYGOTE; HUMAN; METABOLISM; METHODOLOGY; MOLECULAR GENETICS; MUTAGENESIS;

CLONING, MOLECULAR; DIDEOXYNUCLEOSIDES; DIPHOSPHATES; DNA; DNA-DIRECTED DNA POLYMERASE; HETEROZYGOTE; HUMANS; INORGANIC PYROPHOSPHATASE; MOLECULAR SEQUENCE DATA; MUTAGENESIS; PYROPHOSPHATASES; SEQUENCE ANALYSIS, DNA; TAQ POLYMERASE; THERMOPLASMA;

EID: 0031115901     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/97224pf02     Document Type: Article

References (15)

1. Barnes, W.M. 1992. The fidelity of Taq polymerase catalyzing PCR is improved by an N-terminal deletion. Gene 112:29-35.
2. Braithwaite, D.K. and J. Ito. 1993. Compilation, alignment, and phylogenetic relationships of DNA polymerases. Nucleic Acids Res. 21:787-802.
3. Huibregtse, J.M. and D.R. Engelke. 1988. DNA Sequencing from complex double-stranded templates. DNA Prot. Eng. Techniques 1:39-41.
4. Koop, B.F., L. Rowan, W.-Q. Chen, P. Deshpande, H. Lee and L. Hood. 1993. Sequence length and error analysis of Sequenase® and automated Taq cycle sequencing methods. BioTechniques 14:442-447.
5. Mamone, J.T., S.B. Samols and C.W. Fuller. 1994. Use of the Sequenase PCR product sequencing kit for analysis of 16S rRNA genes from novel microorganisms. Comments 21:34-36.
6. McMahon, G., E. Davis and G.N. Wogan. 1987. Characterization of c-Ki-ras oncogene alleles by direct sequencing of enzymatically amplified DNA from carcinogen-induced tumors. Proc. Natl. Acad. Sci. USA 84:4974-4978.
7. Murray, V. 1989. Improved double-stranded DNA sequencing using the linear polymerase chain reaction. Nucleic Acids Res. 17:8889.
8. Reddy, P., A. Peterkofsky and K. McKenney. 1989. Hyperexpression and purification of Escherichia coli adenylate cyclase using a vector designed for expression of lethal gene products. Nucleic Acids Res. 17:10473-10488.
9. Richter, O.-M.H. and G. Schäfer. 1992. Purification and enzymatic characterization of the cytoplasmic pyrophosphatase from the thermoacidophilic archaebacterium Thermoplasma acidophilum. Eur. J. Biochem. 209:343-349.
10. Richter, O.-M.H. and G. Schäfer. 1992. Cloning and sequencing of the gene for the cytoplasmic inorganic pyrophosphatase from the thermoacidophilic archaebacterium Thermoplasma acidophilum. Eur. J. Biochem. 209:351-355.
11. Sambrook, J., E.F. Fritsch and T. Maniatis. 1989. Molecular Cloning: A Laboratory Manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
12. Sanger, F., S. Nicklen and A.R. Coulson. 1977. DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA 74:5463-5467.
13. Tabor, S. and C.C. Richardson. 1987. DNA sequence analysis with a modified bacteriophage T7 DNA polymerase. Proc. Natl. Acad. Sci. USA 84:4767-4771.
14. Tabor, S. and C.C. Richardson. 1990. DNA sequence analysis with a modified bacteriophage T7 DNA polymerase: effect of pyrophosphorolysis and metal ions. J. Biol. Chem. 265:8322-8328.
15. Tabor, S. and C.C. Richardson. 1995. A single hydroxyl moiety in Pol 1-type DNA polymerases is responsible for distinguishing between deoxy-and dideoxyribonucleotides. Proc. Natl. Acad. Sci. USA 92:6339-6343.