Molecular cloning, expression, and site-directed mutagenesis of inorganic pyrophosphatase from Thermus thermophilus HB8

Journal of Biochemistry

Volumn 124, Issue 1, 1998, Pages 79-88

  Satoh, Takanori ^a   Samejima, Tatsuya ^a   Watanabe, Machiko ^a   Nogi, Shin Ichi ^a   Takahashi, Yoshimasa ^a   Kaji, Hiroyuki ^a,d   Teplyakov, Alexei ^b   Obmolova, Galya ^b   Kuranova, Inna ^c   Ishii, Keisuke ^a,e  

^a AOYAMA GAKUIN UNIVERSITY   (Japan)

^b EUROPEAN MOLECULAR BIOLOGY LABORATORY   (Germany)

^c SHUBNIKOV INSTITUTE OF CRYSTALLOGRAPHY   (Russian Federation)

^d TOKYO METROPOLITAN UNIVERSITY   (Japan)

^e SHINO TEST CORPORATION   (Japan)

Author keywords

Amino acid sequence; Inorganic pyrophosphatase; Molecular cloning; Site directed mutagenesis; Thermus thermophilus

Indexed keywords

CYANOGEN BROMIDE; FORMIC ACID; INORGANIC PYROPHOSPHATASE; PLASMID VECTOR; PROTEINASE; TRYPTOPHAN;

ACHROMOBACTER; AMINO ACID SEQUENCE; ARTICLE; MOLECULAR CLONING; MOLECULAR HYBRIDIZATION; MOLECULAR WEIGHT; NONHUMAN; NUCLEOTIDE SEQUENCE; OPEN READING FRAME; POLYMERASE CHAIN REACTION; PROTEIN EXPRESSION; SITE DIRECTED MUTAGENESIS; STAPHYLOCOCCUS AUREUS; THERMOSTABILITY; THERMUS THERMOPHILUS;

ACHROMOBACTER; BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; NEGIBACTERIA; PROKARYOTA; STAPHYLOCOCCUS AUREUS; THERMUS THERMOPHILUS;

EID: 0031824615     PISSN: 0021924X     EISSN: None     Source Type: Journal    
DOI: 10.1093/oxfordjournals.jbchem.a022100     Document Type: Article

References (37)

1. Josse, J. and Wong, S.C.K. (1971) Inorganic pyrophosphatase of Escherichia coli in The Enzymes (Boyer, P.D., ed.) 3rd. Vol. 4, pp. 499-527, Academic Press, New York
2. Cooperman, B.S., Baykov, A.A., and Lahti, R. (1992) Evolutionary conservation of the active site of soluble inorganic pyrophosphatase. Trends Biochem. Sci. 17, 262-266
3. Salminen, T., Käplyä, J., Heikinheimo, P., Kankare, J., Goldman, A., Heinonen, J., Baykov, A.A., Cooperman, B.S., and Lahti, R. (1995) Structure and function analysis of Escherichia coli inorganic pyrophosphatase: Is a hydroxide ion the key to catalysis? Biochemistry 34, 782-791
4. 2+ dependence. Biochemistry 35, 4655-4661
5. Heikinheimo, P., Pohjanjoki, P., Helminen, A., Tasane, M., Cooperman, B.S., Goldman, A., Baykov, A.A., and Lahti, R. (1996) A site-directed mutagenesis study of Saccharomyces cerevisiae pyrophosphatase. Functional conservation of the active site of soluble inorganic pyrophosphatase. Eur. J. Biochem. 239, 138-143
6. Kolakowski, L.F., Schlösser, M., and Cooperman, B.S. (1988) Cloning, molecular characterization and chromosome localization of the inorganic pyrophosphatase (PPA) gene from S. cerevisiae. Nucleic Acids Res. 22, 10441-10452
7. Lahti, R., Pitkäranta, T., Valve, E., Ilta, I., Kukko-Kalske, E., and Heinonen, J. (1988) Cloning and characterization of the gene encoding inorganic pyrophosphatase of Escherichia coli K-12. J. Bacteriol. 170, 5901-5907
8. Ichiba, T., Takenaka, O., Samejima, T., and Hachimori, A. (1990) Primary structure of the inorganic pyrophosphatase from thermophilic bacterium PS-3. J. Biochem. 108, 572-578
9. Maruyama, S., Maeshima, M., Nishimura, M., Aoki, M., Ichiba, T., Sekiguchi, J., and Hachimori, A. (1996) Cloning and expression of the inorganic pyrophosphatase gene from thermophilic bacterium PS-3. Biochem. Mol. Biol. Int. 40, 679-688
10. Teplyakov, A., Obmoloba, G., Wilson, K.S., Ishii, K., Kaji, H., Samejima, T., and Kuranova, I. (1994) Crystal structure of inorganic pyrophosphatase from Thermus thermophilus. Protein Sci. 3, 1098-1107
11. Harutyunyan, E.H., Oganessyan, V.Y., Oganessyan, N.N., Avaeva, S.M., Nazarova, T.I., Vorobyeva, N.N., Kurilova, S.A., Huber, R., and Mather, T. (1997) Crystal structure of holo inorganic pyrophosphatase from Escherichia coli at 1.9 Å resolution. Mechanism of hydrolysis. Biochemistry 36, 7754-7760
12. Kankare, J., Salminen, T., Lahti, R., Cooperman, B.S., Baykov, A.A., and Goldman, A. (1996) Crystallographic identification of metal-binding sites in Escherichia coli inorganic pyrophosphatase. Biochemistry 35, 4670-4677
13. Heikinheimo, P., Lehtonen, J., Baykov, A.A., Lahti, R., Cooperman, B.S., and Goldman, A. (1996) The structural basis for pyrophosphatase catalysis. Structure 4, 1491-1508
14. Salminen, T., Teplyakov, A., Kankare, J., Cooperman, B.S., Lahti, R., and Goldman, A. (1996) An unusual route to thermostability disclosed by the comparison of Thermus thermophilus and Escherichia coli inorganic pyrophosphatases. Protein Sci. 5, 1014-1025
15. Obmolova, G., Kuranova, I., and Teplyakov, A. (1993) Purification, crystallization and preliminary X-ray analysis of inorganic pyrophosphatase from Thermus thermophilus. J. Mol. Biol. 232, 312-313
16. Friedman, M., Krull, L.H., and Cavins, J.F. (1970) The chromatographic determination of cystine and cysteine residues in protein as s-beta-(4-pyridylethyl) cysteine. J. Biol. Chem. 245, 3868-3871
17. Chung, C.T., Niemela, S.L., and Miller, R.H. (1989) One-step preparation of competent Escherichia coli: transformation and storage of bacterial cells in the same solution. Proc. Natl. Acad. Sci. USA 86, 2172-2175
18. Yoshizaki, F., Oshima, T., and Imahori, K. (1971) Studies on phosphoglucomutase from an extreme thermophile, Flavobacterium thermophilum HB8. I. Thermostability and other enzymatic properties. J. Biochem. 69, 1083-1089
19. Maniatis, T., Fritsh, E.F., and Sambrook, F.J. (1982) Molecular Cloning: A Laboratory Manual, pp. 196, Cold Spring Harbor Laboratory, Cold Spring Habor, New York
20. Innis, M.A., Gelfand, D.H., Sninsky, J.J., and White T.J. (1990) PCR Protocols. A Guide to Methods and Application, Academic Press, San Diego
21. Hachimori, A., Takeda, A., Kaibuchi, M., Ohkawara, N., and Samejima, T. (1975) Purification and characterization of inorganic pyrophosphatase from Bacillus stearothermophilus. J. Biochem. 77, 1177-1183
22. Peel, J.H. and Loughman, B.C. (1957) Some observations on the role of copper ions in the reduction of phosphomolybdate by ascorbic acid and their application in the determination of inorganic pyrophosphate. Biochem. J. 65, 709-716
23. Lowry, O.H., Rosebrough, N.J., Farr, A.L., and Randall, R.J. (1951) Protein measurement with the Folin phenol reagent. J. Mol. Chem. 193, 265-275
24. Laemmli, U.K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680-685
25. Höhne, W.E., Wessner, H., Kuranova, I.P., and Obmolova, G.V. (1988) Kinetics characterization of a thermostable inorganic pyrophosphatase from Thermus thermophilus. Biomed. Biochim. Acta 47, 941-947
26. Kaneko, S., Ichiba, T., Hirano, N., and Hachimori, A. (1993) Modification of tryptophan 149 of inorganic pyrophosphatase from Escherichia coli. Int. J. Biochem. 25, 233-238
27. Negi, T., Samejima, T., and Irie, M. (1972) Studies on the tryptophan residues of yeast inorganic pyrophosphatase in relation to the enzymatic activity. J. Biochem. 71, 29-37
28. Kaneko, S., Ichiba, T., Hirano, N., and Hachimori, A. (1991) Modification of a single tryptophan of the inorganic pyrophosphatase from thermophilic bacterium PS-3: possible involvement in its substrate binding. Biochim. Biophys. Acta 1077, 281-284
29. Xu, J., Seki, M., Denda, K., and Yoshida, M. (1991) Molecular cloning of phosphofructokinase 1 gene from a thermophilic bacterium, Thermus thermophilus. Biochem. Biophys. Res. Commun. 176, 1313-1318
30. Kato, R. and Kuramitsu, S. (1993) RecA protein from an extremely thermophilic bacterium, Thermus thermophilus HB8. J. Biochem. 114, 926-929
31. Motohashi, K., Yohda, M., Endo, I., and Yoshida, M. (1996) A novel factor required for the assembly of the DnaK and DnaJ chaperones of Thermus thermophilus. J. Biol. Chem. 271, 17343-17348
32. Amada, K., Yohda, M., Odaka, M., Endo, I., Ishii, N., Taguchi, H., and Yoshida, M. (1995) Molecular cloning, expression, and characterization of chaperonin-60 and chaperonin-10 from a thermophilic bacterium, Thermus thermophilus. J. Biochem. 118, 347-354
33. Hartmann, R.K. and Erdmann, V.A. (1989) Thermus thermophilus 16S rRNA is transcribed from an isolated transcription unit. J. Bacteriol. 171, 2933-2941
34. Richter, O.M.H. and Schäfer, G. (1992) Cloning and sequencing of the gene for the cytoplasmic inorganic pyrophosphatase from the thermoacidophilic archaebacterium Thermoplasma acidophilum. Eur. J. Biochem. 209, 351-355
35. Stark, M.R. and Milner, J.S. (1989) Cloning and analysis of the Kluyveromyces lactis TRP1 gene: a chromosomal locus flanked by genes encoding inorganic pyrophosphatase and histone H3. Yeast 5, 35-50
36. Kawasaki, I., Adachi, N., and Ikeda, H. (1990) Nucleotide sequence of S. pombe inorganic pyrophosphatase. Nucleic Acids Res. 18, 5888
37. Kieber, J.J. and Signer, E.R. (1991) Cloning and characterization of an inorganic pyrophosphatase gene from Arabidopsis thaliana. Plant Mol. Biol. 16, 345-348