A colorimetric assay for phosphate to measure amplicon accumulation in polymerase chain reaction

Analytical Biochemistry

Volumn 254, Issue 1, 1997, Pages 18-22

  Gibson, Neil J ^a   Newton, Clive R ^a   Little, Stephen ^a  

^a ASTRAZENECA   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

COLOR; COLORIMETRY;

ALLELIC VARIANTS; AMPLIFICATION REFRACTORY MUTATION SYSTEMS; COLOR DEVELOPMENT; COLOR GENERATION; COLORIMETRIC ASSAYS; COLORIMETRIC METHODS; CYSTIC FIBROSIS; MIXING REAGENTS;

POLYMERASE CHAIN REACTION;

PHOSPHATE; PRIMER DNA;

ARTICLE; COLORIMETRY; CYSTIC FIBROSIS; DNA REPLICATION; GENE MUTATION; GEOBACILLUS STEAROTHERMOPHILUS; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SPECTROPHOTOMETRY; THERMUS THERMOPHILUS;

GEOBACILLUS STEAROTHERMOPHILUS; THERMUS THERMOPHILUS;

EID: 0031543414     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1006/abio.1997.2324     Document Type: Article

References (18)

1. Newton C. R., Graham A., Heptinstall L. E., Powell S. J., Summers C., Kalsheker N. Nucleic Acids Res. 17:1989;2503-2515.
2. Riordan J. R., Rommens J. M., Kerem B., Alon N., Rozmahel R., Grzelczak Z. Science. 245:1989;1066-1073.
3. Bertina R. M., Koeleman B. P. C., Koster T., Rosendaal F. R., Dirven R. J., de Ronde H., van der Velden P. A., Reitsma P. H. Nature. 369:1994;64-67.
4. Burns D. T., Chimpalee D., Chimpalee N., Ittipornkul S. Anal. Chim. Acta. 254:1991;197-200.
5. Hoenig M., Lee R. J., Ferguson D. F. J. Biochem. Biophys. Methods. 19:1989;249-252.
6. Geladopoulos T. P., Sotiroudis T. D., Evangelopoulos A. E. Anal. Biochem. 192:1991;112-116.
7. Baykov A. A., Evtushenko O. A., Avaeva S. M. Anal. Biochem. 171:1988;266-270.
8. Ekman P., Jager O. Anal. Biochem. 214:1993;138-141.
9. Nyrén P., Pettersson B., Uhlén M. Anal. Biochem. 208:1993;171-175.
10. Wu D. Y., Ugozzoli L., Pal B. K., Wallace R. B. Proc. Natl. Acad. Sci. USA. 86:1989;2757-2760.
11. Sommer S. S., Cassady J. D., Sobell M. S., Bottema C. D. K. Mayo Clin. Proc. 64:1989;1361-1372.
12. Kwok S., Higuchi R. Nature. 339:1989;237-238.
13. Newton C. R., Graham A. PCR - Introduction to Biotechniques. 1997;Bios Scientific, Oxford.
14. MacCallum L. J. PCR: Essential Data. 1995;Wiley, West Sussex. p. 99-127.
15. Gibson N. J., Gillard H. L., Whitcombe D., Ferrie R. M., Newton C. R., Little S. Clin. Chem. 43:1997;1336-1341.
16. Holland P. M., Abramson R. D., Watson R., Gelfand D. H. Proc. Natl. Acad. Sci. USA. 88:1991;7276-7280.
17. Abdallah A. M., Khalifa M. E., Akl M. A. Anal. Chim. Acta. 251:1991;207-213.
18. Brownie J., Shawcross S., Theaker J., Whitcombe D., Ferrie R., Newton C., Little S. Nucleic Acids Res. 25:1997;3235-3241.