Dermal fibroblast-mediated BMP2 therapy to accelerate bone healing in an equine osteotomy model

Journal of Orthopaedic Research

Volumn 28, Issue 3, 2010, Pages 403-411

  Ishihara, Akikazu ^a   Zekas, Lisa J ^a   Litsky, Alan S ^a   Weisbrode, Steven E ^a   Bertone, Alicia L ^a  

^a OHIO STATE UNIVERSITY   (United States)

Author keywords

Adenovirus; BMP2; Bone healing; Dermal fibroblast

Indexed keywords

ADENOVIRUS VECTOR; BONE MORPHOGENETIC PROTEIN 2; GLIAL FIBRILLARY ACIDIC PROTEIN; GREEN FLUORESCENT PROTEIN; RECOMBINANT PROTEIN;

ANIMAL MODEL; ARTICLE; BONE DEFECT; BONE MINERALIZATION; CELL BASED GENE THERAPY; CELL DIFFERENTIATION; CELL TRANSPLANTATION; CONTROLLED STUDY; FRACTURE; FRACTURE HEALING; GENE EXPRESSION; METACARPAL BONE; METATARSAL OSTEOTOMY; NONHUMAN; PRIORITY JOURNAL; SKIN FIBROBLAST;

ANIMALS; BONE AND BONES; BONE MORPHOGENETIC PROTEIN 2; CELL TRANSPLANTATION; DERMIS; FEASIBILITY STUDIES; FIBROBLASTS; GENETIC ENGINEERING; HORSES; INJECTIONS; METACARPAL BONES; METATARSAL BONES; OSTEOTOMY; TRANSDUCTION, GENETIC; WOUND HEALING;

EID: 73949150050     PISSN: 07360266     EISSN: None     Source Type: Journal    
DOI: 10.1002/jor.20978     Document Type: Article

References (32)

1. Evans CH, Ghivizzani SC, Robbins PD. 2009. Orthopedic gene therapy in 2008. Mol Ther 17:231-244.
2. Zachos T, Diggs A, Weisbrode S, et al. 2007. Mesenchymal stem cell-mediated gene delivery of bone morphogenetic protein-2 in an articular fracture model. Mol Ther 15:1543-1550.
3. Zilberman Y, Kallai I, Gafni Y, et al. 2008. Fluorescence molecular tomography enables in vivo visualization and quantification of nonunion fracture repair induced by genetically engineered mesenchymal stem cells. J Orthop Res 26:522-530.
4. Apel A, Groth A, Schlesinger S, et al. 2009. Suitability of human mesenchymal stem cells for gene therapy depends on the expansion medium. Exp Cell Res 315:498-507.
5. Takahashi K, Tanabe K, Ohnuki M, et al. 2007. Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Cell 131:861-872.
6. Hanna J, Wernig M, Markoulaki S, et al. 2007. Treatment of sickle cell anemia mouse model with iPS cells generated from autologous skin. Science 318:1920-1923.
7. Mauritz C, Schwanke K, Reppel M, et al. 2008. Generation of functional murine cardiac myocytes from induced pluripotent stem cells. Circulation 118:507-517.
8. Wernig M, Zhao JP, Pruszak J, et al. 2008. Neurons derived from reprogrammed fibroblasts functionally integrate into the fetal brain and improve symptoms of rats with Parkinson's disease. Proc Natl Acad Sci USA 105:5856-5861.
9. Hirata K, Tsukazaki T, Kadowaki A, et al. 2000. Transplantation of skin fibroblasts expressing BMP-2 promotes bone repair more effectively than those expressing Runx2. Bone 32:502-512.
10. Hirata K, Mizuno A, Yamaguchi A. 2007. Transplantation of skin fibroblasts expressing BMP-2 contributes to the healing of critical-sized bone defects. J Bone Miner Metab 25:6-11.
11. Krebsbach PH, Gu K, Franceschi RT, et al. 2000. Gene therapy-directed osteogenesis: BMP-7-transduced human fibroblasts form bone in vivo. Hum Gene Ther 11:1201-1210.
12. Rutherford RB, Moalli M, Franceschi RT, et al. 2002. Bone morphogenetic protein-transduced human fibroblasts convert to osteoblasts and form bone in vivo. Tissue Eng 8:441-452.
13. Lattanzi W, Parrilla C, Fetoni A, et al. 2008. Ex vivo-transduced autologous skin fibroblasts expressing human Lim mineralization protein-3 efficiently form new bone in animal models. Gene Ther 15:1330-1343.
14. Egermann M, Lill CA, Griesbeck K, et al. 2006. Effect of BMP-2 gene transfer on bone healing in sheep. Gene Ther 13:1290-1299.
15. Ishihara A, Shields KM, Litsky AS, et al. 2008. Osteogenic gene regulation and relative acceleration of healing by adenoviral-mediated transfer of human BMP-2 or -6 in equine osteotomy and ostectomy models. J Orthop Res 26:764-771.
16. Bertone AL, Pittman DD, Bouxsein ML, et al. 2004. Adenoviral-mediated transfer of human BMP-6 gene accelerates healing in a rabbit ulnar osteotomy model. J Orthop Res 22:1261-1270.
17. Betz OB, Betz VM, Nazarian A, et al. 2007. Delayed administration of adenoviral BMP-2 vector improves the formation of bone in osseous defects. Gene Ther 14:1039-1044.
18. Chen D, Zhao M, Mundy GR. 2004. Bone morphogenetic proteins. Growth Factors 22:233-241.
19. Mehlhorn AT, Niemeyer P, Kaschte K, et al. 2007. Differential effects of BMP-2 and TGF-beta1 on chondrogenic differentiation of adipose derived stem cells. Cell Prolif 40:809-823.
20. Glowacki J, Yates KE, Maclean R, et al. 2005. In vitro engineering of cartilage: effects of serum substitutes, TGF-beta, and IL-1alpha. Orthod Craniofac Res 8:200-208.
21. Liu X, Zhou G, Liu W, et al. 2007. In vitro formation of lacuna structure by human dermal fibroblasts co-cultured with porcine chondrocytes on a 3D biodegradable scaffold. Biotechnol Lett 29:1685-1690.
22. Zhou S, Glowacki J, Yates KE. 2004. Comparison of TGF-beta/BMP pathways signaled by demineralized bone powder and BMP-2 in human dermal fibroblasts. J Bone Miner Res 19:1732-1741.
23. Hwang NS, Varghese S, Lee HJ, et al. 2008. In vivo commitment and functional tissue regeneration using human embryonic stem cell-derived mesenchymal cells. Proc Natl Acad Sci USA 105:20641-20646.
24. McCullough KA, Waits CA, Garimella R, et al. 2007. Immunohistochemical localization of bone morphogenetic proteins (BMPs) 2, 4, 6, and 7 during induced heterotopic bone formation. J Orthop Res 25:465-472.
25. Susperregui AR, Viñals F, Ho PW, et al. 2008. BMP-2 regulation of PTHrP and osteoclastogenic factors during osteoblast differentiation of C2C12 cells. J Cell Physiol 216:144-152.
26. Abe E, Yamamoto M, Taguchi Y, et al. 2000. Essential requirement of BMPs-2/4 for both osteoblast and osteoclast formation in murine bone marrow cultures from adult mice: Antagonism by noggin. J Bone Miner Res 15:663-673.
27. Yoon E, Dhar S, Chun DE, et al. 2007. In vivo osteogenic potential of human adipose-derived stem cells/poly lactide-coglycolic acid constructs for bone regeneration in a rat criticalsized calvarial defect model. Tissue Eng 13:619-627.
28. Xiao G, Gopalakrishnan R, Jiang D, et al. 2002. Bone morphogenetic proteins, extracellular matrix, and mitogen-activated protein kinase signaling pathways are required for osteoblast-specific gene expression and differentiation in MC3T3-E1 cells. J Bone Miner Res 17:101-110.
29. Gersbach CA, Guldberg RE, García AJ. 2007. In vitro and in vivo osteoblastic differentiation of BMP-2- and Runx2-engineered skeletal myoblasts. J Cell Biochem 100:1324-1336.
30. Muschler GF, Midura RJ. 2002. Connective tissue progenitors: practical concepts for clinical applications. Clin Orthop Relat Res 395:66-80.
31. Govender S, Csimma C, Genant HK, et al. 2002. Recombinant human bone morphogenetic protein-2 for treatment of open tibial fractures: a prospective, controlled, randomized study of four hundred and fifty patients. J Bone Joint Surg Am 84-A:2123-2134.
32. Swiontkowski MF, Aro HT, Donell S, et al. 2006. Recombinant human bone morphogenetic protein-2 in open tibial fractures. A subgroup analysis of data combined from two prospective randomized studies. J Bone Joint Surg Am 88:1258-1265.