Stable knockdown of Aurora-A by vector-based RNA interference in human esophageal squamous cell carcinoma cell line inhibits tumor cell proliferation, invasion and enhances apoptosis

Cancer Biology and Therapy

Volumn 8, Issue 19, 2009, Pages 1852-1859

  Wang, Xiaoxia ^a,b   Dong, Lijia ^a   Xie, Jun ^b   Tong, Tong ^a   Zhan, Qimin ^a  

^a PEKING UNION MEDICAL COLLEGE   (China)

^b SHANXI MEDICAL UNIVERSITY   (China)

Author keywords

Apoptosis; Aurora A; Esophageal squamous cell carcinoma (ESCC); Invasion; RNA interference

Indexed keywords

AURORA A KINASE; CISPLATIN;

APOPTOSIS; ARTICLE; CANCER CELL CULTURE; CANCER INVASION; CELL PROLIFERATION; CONTROLLED STUDY; ESOPHAGEAL SQUAMOUS CELL CARCINOMA; GENE SILENCING; HUMAN; HUMAN CELL; PROTEIN FUNCTION; RNA INTERFERENCE;

APOPTOSIS; BLOTTING, WESTERN; CELL GROWTH PROCESSES; CELL LINE, TUMOR; ESOPHAGEAL NEOPLASMS; GENE KNOCKDOWN TECHNIQUES; HUMANS; NEOPLASMS, SQUAMOUS CELL; PROTEIN-SERINE-THREONINE KINASES; RNA INTERFERENCE; RNA, SMALL INTERFERING; TRANSFECTION;

EID: 73549102150     PISSN: 15384047     EISSN: 15558576     Source Type: Journal    
DOI: 10.4161/cbt.8.19.9550     Document Type: Article

References (31)

1. Jemal A, Murray T, Ward E, Samuels A, Tiwari RC, Ghafoor A, et al. Cancer statistics, 2005. CA Cancer J Clin 2005; 55:10-30.
2. Enzinger PC, Mayer RJ. Esophageal cancer. N Engl J Med 2003; 349:2241-52.
3. Portale G, Hagen JA, Peters JH, Chan LS, DeMeester SR, Gandamihardja TA, DeMeester TR. Modern 5-year survival of resectable esophageal adenocarcinoma: single institution experience with 263 patients. J Am Coll Surg 2006; 202:588-96.
4. Gautschi O, Heighway J, Mack PC, Purnell PR, Lara PN Jr, Gandara DR. Aurora kinases as anticancer drug targets. Clin Cancer Res 2008; 14:1639-48.
5. Zhou H, Kuang J, Zhong L, Kuo WL, Gray JW, Sahin A, et al. Tumour amplified kinase STK15/BTAK induces centrosome amplification, aneuploidy and transformation. Nat Genet 1998; 20:189-93.
6. Bischoff JR, Anderson L, Zhu Y, Mossie K, Ng L, Souza B, et al. A homologue of Drosophila aurora kinase is oncogenic and amplified in human colorectal cancers. EMBO J 1998; 17:3052-65.
7. Landen CN Jr, Lin YG, Immaneni A, Deavers MT, Merritt WM, Spannuth WA, et al. Overexpression of the centrosomal protein Aurora-A kinase is associated with poor prognosis in epithelial ovarian cancer patients. Clin Cancer Res 2007; 13:4098-104.
8. Sen S, Zhou H, Zhang RD, Yoon DS, Vakar-Lopez F, Ito S, et al. Amplification/overexpression of a mitotic kinase gene in human bladder cancer. J Natl Cancer Inst 2002; 94:1320-9.
9. Xu HT, Ma L, Qi FJ, Liu Y, Yu JH, Dai SD, et al. Expression of serine threonine kinase 15 is associated with poor differentiation in lung squamous cell carcinoma and adenocarcinoma. Pathol Int 2006; 56:375-80.
10. Manfredi MG, Ecsedy JA, Meetze KA, Balani SK, Burenkova O, Chen W, et al. Antitumor activity of MLN8054, an orally active small-molecule inhibitor of Aurora A kinase. Proc Natl Acad Sci USA 2007; 104:4106-11.
11. Vader G, Lens SM. The Aurora kinase family in cell division and cancer. Biochim Biophys Acta 2008; 1786:60-72.
12. Wang XX, Liu R, Jin SQ, Fan FY, Zhan QM. Overexpression of Aurora-A kinase promotes tumor cell proliferation and inhibits apoptosis in esophageal squamous cell carcinoma cell line. Cell Res 2006; 16:356-66.
13. Tanaka E, Hashimoto Y, Ito T, Okumura T, Kan T, Watanabe G, et al. The clinical significance of Aurora-A/STK15/BTAK expression in human esophageal squamous cell carcinoma. Clin Cancer Res 2005; 11:1827-34.
14. Tong T, Zhong Y, Kong J, Dong L, Song Y, Fu M. Overexpression of Aurora-A contributes to malignant development of human esophageal squamous cell carcinoma. Clin Cancer Res 2004; 10:7304-10.
15. Kim D, Rossi J. RNAi mechanisms and applications. Biotechniques 2008; 44:613-6.
16. Elbashir SM, Harborth J, Lendeckel W, Yalcin A, Weber K, Tuschl T. Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells. Nature 2001; 411:494-8.
17. Sui G, Soohoo C, Affar el B, Gay F, Shi Y, Forrester WC, Shi Y. A DNA vector-based RNAi technology to suppress gene expression in mammalian cells. Proc Natl Acad Sci USA 2002; 99:5515-20.
18. Wu MT, Wu RH, Hung CF, Cheng TL, Tsai WH, Chang WT. Simple and efficient DNA vector-based RNAi systems in mammalian cells. Biochem Biophys Res Commun 2005; 330:53-61.
19. Izquierdo M. Short interfering RNAs as a tool for cancer gene therapy. Cancer Gene Ther 2005; 12:217-27.
20. Agami R. RNAi and related mechanisms and their potential use for therapy. Curr Opin Chem Biol 2002; 6:829-34.
21. Zhang M, Zhou Y, Xie C, Zhou F, Chen Y, Han G, Zhang WJ. STAT6 specific shRNA inhibits proliferation and induces apoptosis in colon cancer HT-29 cells. Cancer Lett 2006; 243:38-46.
22. Paddison PJ, Caudy AA, Sachidanandam R, Hannon GJ. Short hairpin activated gene silencing in mammalian cells. Methods Mol Biol 2004; 265:85-100.
23. Vermeulen A, Behlen L, Reynoids A, Wolfson A, Marshall WS, Karpilow J, Khvorova A. The contributions of dsRNA structure to Dicer specificity and efficiency. RNA 2005; 11:674-82.
24. Lu PY, Xie F, Woodle MC. In vivo application of RNA interference: from functional genomics to therapeutics. Adv Genet 2005; 54:117-42.
25. Hanahan D, Weinberg RA. The hallmarks of cancer. Cell 2000; 100:57-70.
26. Fischer U, Schulze-Osthoff K. New approaches and therapeutics targeting apoptosis in disease. Pharmacol Rev 2005; 57:187-215.
27. Sjostrom J, Bergh J. How apoptosis is regulated, and what goes wrong in cancer. BMJ 2001; 322:1538-9.
28. Hata T, Furukawa T, Sunamura M, Egawa S, Motoi F, Ohmura N, et al. RNA interference targeting aurora kinase suppresses tumor growth and enhances the taxane chemosensitivity in human pancreatic cancer cells. Cancer Res 2005; 65:2899-905.
29. Tanaka E, Hashimoto Y, Ito T, Kondo K, Higashiyama M, Tsunoda S, et al. The suppression of aurora-A/STK15/BTAK expression enhances chemosensitivity to docetaxel in human esophageal squamous cell carcinoma. Clin Cancer Res 2007; 13:1331-40.
30. Lowe SW, Ruley HE, Jacks T, Housman DE. p53-dependent apoptosis modulates the cytotoxicity of anticancer agents. Cell 1993; 74:957-67.
31. Guan Z, Wang XR, Zhu XF, Huang XF, Xu J, Wang LH, et al. Aurora-A, a negative prognostic marker, increases migration and decreases radiosensitivity in cancer cells. Cancer Res 2007; 67:10436-44.