The human c-fos and TNFα AU-rich elements show different effects on mRNA abundance and protein expression depending on the reporter in the yeast Pichia pastoris

Yeast

Volumn 27, Issue 1, 2010, Pages 1-9

  Lautz, Thomas ^a,b   Stahl, Ulf ^a   Lang, Christine ^a  

^a TECHNISCHE UNIVERSITÄT BERLIN   (Germany)

^b NewLab Bioquality GmbH   (Germany)

Author keywords

AU rich elements; mRNA degradation; Pichia pastoris; Protein expression

Indexed keywords

COMPLEMENTARY DNA; MESSENGER RNA; PROTEIN C FOS; TUMOR NECROSIS FACTOR ALPHA;

3' UNTRANSLATED REGION; ARTICLE; CONTROLLED STUDY; GENETIC REGULATION; NONHUMAN; NUCLEOTIDE SEQUENCE; PICHIA PASTORIS; POINT MUTATION; PRIORITY JOURNAL; PROTEIN EXPRESSION; REPORTER GENE; RNA SEQUENCE;

ADENINE; FUNGAL PROTEINS; GENE EXPRESSION REGULATION; GENES, REPORTER; HUMANS; PICHIA; PROTO-ONCOGENE PROTEINS C-FOS; RNA, MESSENGER; TUMOR NECROSIS FACTOR-ALPHA; URACIL;

PICHIA PASTORIS;

EID: 73449139763     PISSN: 0749503X     EISSN: 10970061     Source Type: Journal    
DOI: 10.1002/yea.1726     Document Type: Article

References (23)

1. Barreau C, Paillard L, Osborne HB. 2006. AU-rich elements and associated factors: are there unifying principles? Nucleic Acids Res 33: 7138-7150.
2. Boettner M, Prinz B, Holz C, et al. 2002. High-throughput screening for expression of heterologous proteins in the yeast Pichia pastoris. J Biotechnol 99: 51-62.
3. Chabanon H, Mickleburgh I, Burtle B, et al. 2005. An AU-rich stem-loop structure is a critical feature of the perinuclear localization signal of c-myc mRNA. Biochem J 392: 475-483.
4. Chen CY, Gherzi R, Ong SE, et al. 2001. AU binding proteins recruit the exosome to degrade ARE-containing mRNAs. Cell 107: 451-464.
5. Chen CY, Shyu AB. 1995. AU-rich elements: characterization and importance in mRNA degradation. Trends Biochem Sci 20: 465-470.
6. Chen CY, Xu N, Shyu AB. 1995. mRNA decay mediated by two distinct AU-rich elements from c-fos and granulocyte-macrophage colony-stimulating factor transcripts: different deadenylation kinetics and uncoupling from translation. Mol Cell Biol 15: 5777-5788.
7. Cok SJ, Acton SJ, Morrison AR. 2003. The proximal region of the 3′-untranslated region of cyclooxygenase-2 is recognized by a multimeric protein complex containing HuR, TIA-1, TIAR, and the heterogeneous nuclear ribonucleoprotein U. J Biol Chem 278: 36157-36162.
8. Duttagupta R, Vasudevan S, Wilusz CJ, Peltz SW. 2003. A yeast homologue of Hsp70, Ssa1p, regulates turnover of the MFA2 transcript through its AU-rich 3′ untranslated region. Mol Cell Biol 23: 2623-2632.
9. Fan XC, Steitz JA. 1998. Overexpression of HuR, a nuclear-cytoplasmic shuttling protein, increases the in vivo stability of ARE-containing mRNAs. EMBO J 17: 3448-3460.
10. Fialcowitz EJ, Brewer BY, Keenan BP, Wilson GM. 2005. A hairpin-like structure within an AU-rich mRNA-destabilizing element regulates trans-factor binding selectivity and mRNA decay kinetics. J Biol Chem 280: 22406-22417.
11. Heinemann U, Frevert J, Hofmann K, et al. 2000. An integrated approach to structural genomics. Prog Biophys Mol Biol 73: 347-362.
12. Katsanou V, Papadaki O, Milatos S, et al. 2005. HuR as a negative posttranscriptional modulator in inflammation. Mol Cell 19: 777-789.
13. Laemmli UK. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680-685.
14. Paschoud S, Dogar AM, Kuntz C, et al. 2006. Destabilization of interleukin-6 mRNA requires a putative RNA stem-loop structure, an AU-rich element and the RNA-binding protein AUF1. Mol Cell Biol 26: 8228-8241.
15. Piecyk M, Wax S, Beck AR, et al. 2000. TIA-1 is a translational silencer that selectively regulates the expression of TNFα. EMBO J 19: 4154-4163.
16. Shaw G, Kamen R. 1986. A conserved AU sequence from the 3′ untranslated region of GM-CSF mRNA mediates selective mRNA degradation. Cell 46: 659-667.
17. Svoboda P, Di Cara A. 2006. Hairpin RNA: a secondary structure of primary importance. Cell Mol Life Sci 63: 901-908.
18. Vasudevan S, Peltz SW. 2001. Regulated ARE-mediated mRNA decay in Saccharomyces cerevisiae. Mol Cell 7: 1191-1200.
19. Wilson GM, Sutphen K, Chuang Ky, Brewer G. 2001. Folding of A + U-rich RNA elements modulates AUF1 binding. Potential roles in regulation of mRNA turnover. J Biol Chem 276: 8695-8704.
20. Xu N, Chen CY, Shyu AB. 1997. Modulation of the fate of cytoplasmic mRNA by AU-rich elements: key sequence features controlling mRNA deadenylation and decay. Mol Cell Biol 17: 4611-4621.
21. Zhang T, Kruys V, Huez G, Gueydan C. 2002. AU-rich elementmediated translational control: complexity and multiple activities of trans-activating factors. Biochem Soc Trans 30: 952-958.
22. Zubiaga AM, Belasco JG, Greenberg ME. 1995. The nonamer UUAUUUAUU is the key AU-rich sequence motif that mediates mRNA degradation. Mol Cell Biol 15: 2219-2230.
23. Zuker M. 2003. Mfold web server for nucleic acid folding and hybridization prediction. Nucleic Acids Res 31: 3406-3415.