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Volumn 37, Issue 17, 2009, Pages

Preparation of selective and segmentally labeled single-stranded DNA for NMR by self-primed PCR and asymmetrical endonuclease double digestion

Author keywords

[No Author keywords available]

Indexed keywords

ENDONUCLEASE; ISOTOPE; POLYDEOXYRIBONUCLEOTIDE SYNTHASE; SINGLE STRANDED DNA;

EID: 73449107716     PISSN: 03051048     EISSN: 13624962     Source Type: Journal    
DOI: 10.1093/nar/gkp540     Document Type: Article
Times cited : (12)

References (70)
  • 1
    • 0347722841 scopus 로고    scopus 로고
    • Heteronuclear multidimensional NMR experiments for the structure determination of proteins in solution employing pulsed field gradients
    • Sattler, M., Schleucher, J. and Griesinger, C. (1999) Heteronuclear multidimensional NMR experiments for the structure determination of proteins in solution employing pulsed field gradients. Prog. NMR Spec., 34, 93-158.
    • (1999) Prog. NMR Spec. , vol.34 , pp. 93-158
    • Sattler, M.1    Schleucher, J.2    Griesinger, C.3
  • 2
    • 3943074512 scopus 로고    scopus 로고
    • Nuclear magnetic resonance spectroscopy of high-molecular-weight proteins
    • Tugarinov, V., Hwang, P.M. and Kay, L.E. (2004) Nuclear magnetic resonance spectroscopy of high-molecular-weight proteins. Annu. Rev. Biochem., 73, 107-146.
    • (2004) Annu. Rev. Biochem. , vol.73 , pp. 107-146
    • Tugarinov, V.1    Hwang, P.M.2    Kay, L.E.3
  • 3
    • 16244404569 scopus 로고    scopus 로고
    • Molecular fragment replacement approach to protein structure determination by chemical shift and dipolar homology database mining
    • Kontaxis, G., Delaglio, F. and Bax, A. (2005) Molecular fragment replacement approach to protein structure determination by chemical shift and dipolar homology database mining. Methods Enzymol., 394, 42-49.
    • (2005) Methods Enzymol. , vol.394 , pp. 42-49
    • Kontaxis, G.1    Delaglio, F.2    Bax, A.3
  • 4
    • 16244419326 scopus 로고    scopus 로고
    • Determination of protein backbone structures from residual dipolar couplings
    • Prestegard, J.H., Mayer, K.L., Valafar, H. and Benison, G.C. (2005) Determination of protein backbone structures from residual dipolar couplings. Methods Enzymol., 394, 175-185.
    • (2005) Methods Enzymol. , vol.394 , pp. 175-185
    • Prestegard, J.H.1    Mayer, K.L.2    Valafar, H.3    Benison, G.C.4
  • 5
    • 42449102160 scopus 로고    scopus 로고
    • Probing invisible, low-populated states of protein molecules by relaxation dispersion NMR spectroscopy: An application to protein folding
    • Korzhnev, D.M. and Kay, L.E. (2008) Probing invisible, low-populated states of protein molecules by relaxation dispersion NMR spectroscopy: An application to protein folding. Acc. Chem. Res., 41, 442-451.
    • (2008) Acc. Chem. Res. , vol.41 , pp. 442-451
    • Korzhnev, D.M.1    Kay, L.E.2
  • 6
    • 0026744912 scopus 로고
    • Preparation of isotopically labeled ribonu-cleotides for multidimensional NMR-spectroscopy of RNA
    • Batey, R.T., Inada, M., Kujawinski, E., Puglisi, J.D. and Williamson, J.R. (1992) Preparation of isotopically labeled ribonu-cleotides for multidimensional NMR-spectroscopy of RNA. Nucleic Acids Res., 20, 4515-4523.
    • (1992) Nucleic Acids Res. , vol.20 , pp. 4515-4523
    • Batey, R.T.1    Inada, M.2    Kujawinski, E.3    Puglisi, J.D.4    Williamson, J.R.5
  • 8
    • 0029881044 scopus 로고    scopus 로고
    • The use of non-uniform deuterium labelling ['NMR-window'] to study the NMR structure of a 21mer RNA hairpin
    • Foldesi, A., Yamakage, S.I., Nilsson, F.P., Maltseva, T.V. and Chattopadhyaya, J. (1996) The use of non-uniform deuterium labelling ['NMR-window'] to study the NMR structure of a 21mer RNA hairpin. Nucleic Acids Res., 24, 1187-1194.
    • (1996) Nucleic Acids Res. , vol.24 , pp. 1187-1194
    • Foldesi, A.1    Yamakage, S.I.2    Nilsson, F.P.3    Maltseva, T.V.4    Chattopadhyaya, J.5
  • 9
    • 0031576691 scopus 로고    scopus 로고
    • 13C-labeled RNA for NMR studies using enzymatic synthesis
    • 13C-labeled RNA for NMR studies using enzymatic synthesis. J. Am. Chem. Soc., 119, 12100-12108.
    • (1997) J. Am. Chem. Soc. , vol.119 , pp. 12100-12108
    • Tolbert, T.J.1    Williamson, J.R.2
  • 11
    • 0000353016 scopus 로고    scopus 로고
    • The use of NMR methods for conformational studies of nucleic acids
    • Wijmenga, S.S. and van Buuren, B.N.M. (1998) The use of NMR methods for conformational studies of nucleic acids. Prog. NMR Spec., 32, 287-387.
    • (1998) Prog. NMR Spec. , vol.32 , pp. 287-387
    • Wijmenga, S.S.1    van Buuren, B.N.M.2
  • 14
    • 0035223403 scopus 로고    scopus 로고
    • Preparation and use of 2H-labeled RNA oligonucleotides in nuclear magnetic resonance studies
    • Nikonowicz, E.P. (2001) Preparation and use of 2H-labeled RNA oligonucleotides in nuclear magnetic resonance studies. Methods Enzymol., 338, 320-341.
    • (2001) Methods Enzymol. , vol.338 , pp. 320-341
    • Nikonowicz, E.P.1
  • 16
    • 0037077562 scopus 로고    scopus 로고
    • NMR study of 100 kDa HCV IRES RNA using segmental isotope labeling
    • Kim, I., Lukavsky, P.J. and Puglisi, J.D. (2002) NMR study of 100 kDa HCV IRES RNA using segmental isotope labeling. J. Am. Chem. Soc., 124, 9338-9339.
    • (2002) J. Am. Chem. Soc. , vol.124 , pp. 9338-9339
    • Kim, I.1    Lukavsky, P.J.2    Puglisi, J.D.3
  • 17
    • 0036849272 scopus 로고    scopus 로고
    • The apical stem-loop of the hepatitis B virus encapsidation signal folds into a stable tri-loop with two underlying pyrimidine bulges
    • Flodell, S., Schleucher, J., Cromsigt, J., Ippel, H., Kidd-Ljunggren, K. and Wijmenga, S. (2002) The apical stem-loop of the hepatitis B virus encapsidation signal folds into a stable tri-loop with two underlying pyrimidine bulges. Nucleic Acids Res., 30, 4803-4811
    • (2002) Nucleic Acids Res. , vol.30 , pp. 4803-4811
    • Flodell, S.1    Schleucher, J.2    Cromsigt, J.3    Ippel, H.4    Kidd-Ljunggren, K.5    Wijmenga, S.6
  • 19
    • 24744459633 scopus 로고    scopus 로고
    • NMR methods for studying the structure and dynamics of RNA
    • Latham, M.R., Brown, D.J., McCallum, S.A. and Pardi, A. (2005) NMR methods for studying the structure and dynamics of RNA. Chembiochem, 6, 1492-1505.
    • (2005) Chembiochem , vol.6 , pp. 1492-1505
    • Latham, M.R.1    Brown, D.J.2    McCallum, S.A.3    Pardi, A.4
  • 20
    • 24744434584 scopus 로고    scopus 로고
    • Dynamics-based amplification of RNA function and its characterization by using NMR spectroscopy
    • Al-Hashimi, H.M. (2005) Dynamics-based amplification of RNA function and its characterization by using NMR spectroscopy. Chembiochem, 6, 1506-1519.
    • (2005) Chembiochem , vol.6 , pp. 1506-1519
    • Al-Hashimi, H.M.1
  • 21
    • 33747315059 scopus 로고    scopus 로고
    • NMR spectroscopy of ribonucleic acids
    • Flinders, J. and Dieckmann, T. (2006) NMR spectroscopy of ribonucleic acids. Prog. NMR Spec., 48, 137-159.
    • (2006) Prog. NMR Spec. , vol.48 , pp. 137-159
    • Flinders, J.1    Dieckmann, T.2
  • 23
    • 34547820982 scopus 로고    scopus 로고
    • NMR studies of RNA dynamics and structural plasticity using NMR residual dipolar couplings
    • Getz, M., Sun, X.Y., Casiano-Negroni, A., Zhang, Q. and Al-Hashimi, H.M. (2007) NMR studies of RNA dynamics and structural plasticity using NMR residual dipolar couplings. Biopolymers, 86, 384-402.
    • (2007) Biopolymers , vol.86 , pp. 384-402
    • Getz, M.1    Sun, X.Y.2    Casiano-Negroni, A.3    Zhang, Q.4    Al-Hashimi, H.M.5
  • 24
    • 34250728646 scopus 로고    scopus 로고
    • Characterizing the relative orientation and dynamics of RNA A-form helices using NMR residual dipolar couplings
    • Bailor, M.H., Musselman, C., Hansen, A.L., Gulati, K., Patel, D.J. and Al-Hashimi, H.M. (2007) Characterizing the relative orientation and dynamics of RNA A-form helices using NMR residual dipolar couplings. Nature Protocols, 2, 1536-1546.
    • (2007) Nature Protocols , vol.2 , pp. 1536-1546
    • Bailor, M.H.1    Musselman, C.2    Hansen, A.L.3    Gulati, K.4    Patel, D.J.5    Al-Hashimi, H.M.6
  • 25
    • 34547659860 scopus 로고    scopus 로고
    • Recombinant RNA technology: the tRNA scaffold
    • Ponchon, L. and Dardel, F. (2007) Recombinant RNA technology: the tRNA scaffold. Nature Methods, 4, 571-576.
    • (2007) Nature Methods , vol.4 , pp. 571-576
    • Ponchon, L.1    Dardel, F.2
  • 26
    • 48649103008 scopus 로고    scopus 로고
    • Key labeling technologies to tackle sizeable problems in RNA structural biology
    • Dayie, K.T. (2008) Key labeling technologies to tackle sizeable problems in RNA structural biology. Int. J. Mol. Sci., 9, 1214-1240
    • (2008) Int. J. Mol. Sci. , vol.9 , pp. 1214-1240
    • Dayie, K.T.1
  • 30
    • 2442731879 scopus 로고    scopus 로고
    • The application of "the Uppsala NMR-window" concept for conformational analysis of large DNA & RNA by high-field NMR spectroscopy
    • Foldesi, A., Maltseva, T.V. and Chattopadhyaya, J. (1999) The application of "the Uppsala NMR-window" concept for conformational analysis of large DNA & RNA by high-field NMR spectroscopy. Nucleosides and Nucleotides, 18, 1599-1600
    • (1999) Nucleosides and Nucleotides , vol.18 , pp. 1599-1600
    • Foldesi, A.1    Maltseva, T.V.2    Chattopadhyaya, J.3
  • 33
    • 0034388126 scopus 로고    scopus 로고
    • Solution structure of a DNA three-way junction containing two unpaired thymidine bases. Identification of sequence features that decide conformer selection
    • van Buuren, B.N.M., Overmars, F.J.J., Ippel, J.H., Altona, C. and Wijmenga, S.S. (2000) Solution structure of a DNA three-way junction containing two unpaired thymidine bases. Identification of sequence features that decide conformer selection. J. Mol. Biol., 304, 371-383.
    • (2000) J. Mol. Biol. , vol.304 , pp. 371-383
    • van Buuren, B.N.M.1    Overmars, F.J.J.2    Ippel, J.H.3    Altona, C.4    Wijmenga, S.S.5
  • 34
    • 0034917885 scopus 로고    scopus 로고
    • Solid-phase synthesis of selectively labeled DNA: Applications for multidimensional nuclear magnetic resonance spectroscopy
    • Kojima, C., Ono, A.M., Ono, A. and Kainosho, M. (2001) Solid-phase synthesis of selectively labeled DNA: Applications for multidimensional nuclear magnetic resonance spectroscopy. Methods Enzymol., 338, 261-283.
    • (2001) Methods Enzymol. , vol.338 , pp. 261-283
    • Kojima, C.1    Ono, A.M.2    Ono, A.3    Kainosho, M.4
  • 35
    • 0037079686 scopus 로고    scopus 로고
    • Brownian-dynamics simulations of metal-ion binding to four-way junctions
    • van Buuren, B.N.M., Hermann, T., Wijmenga, S.S. and Westhof, E. (2002) Brownian-dynamics simulations of metal-ion binding to four-way junctions. Nucleic Acids Res., 30, 507-514.
    • (2002) Nucleic Acids Res. , vol.30 , pp. 507-514
    • van Buuren, B.N.M.1    Hermann, T.2    Wijmenga, S.S.3    Westhof, E.4
  • 36
    • 0038725129 scopus 로고    scopus 로고
    • Overall structure and sugar dynamics of a DNA dodecamer from homo and heteronuclear dipolar couplings and 31P chemical shift anisotropy
    • Wu, Z.G., Delaglio, F., Tjandra, N., Zhurkin, V.B. and Bax, A. (2003) Overall structure and sugar dynamics of a DNA dodecamer from homo and heteronuclear dipolar couplings and 31P chemical shift anisotropy. J. Biomol. NMR, 26, 297-315.
    • (2003) J. Biomol. NMR , vol.26 , pp. 297-315
    • Wu, Z.G.1    Delaglio, F.2    Tjandra, N.3    Zhurkin, V.B.4    Bax, A.5
  • 37
    • 0347753822 scopus 로고    scopus 로고
    • NMR spectroscopic determination of the solution structure of a branched nucleic acid from residual dipolar couplings by using isotopically labeled nucleotides
    • van Buuren, B.N.M., Schleucher, J., Wittmann, V., Griesinger, C., Schwalbe, H. and Wijmenga, S.S. (2004) NMR spectroscopic determination of the solution structure of a branched nucleic acid from residual dipolar couplings by using isotopically labeled nucleotides. Angew. Chem. Int. Edit., 43, 187-192.
    • (2004) Angew. Chem. Int. Edit. , vol.43 , pp. 187-192
    • van Buuren, B.N.M.1    Schleucher, J.2    Wittmann, V.3    Griesinger, C.4    Schwalbe, H.5    Wijmenga, S.S.6
  • 39
    • 0034911866 scopus 로고    scopus 로고
    • Nuclear magnetic resonance methods to study structure and dynamics of RNA-protein complexes
    • Allen, M., Varani, L. and Varani, G. (2001) Nuclear magnetic resonance methods to study structure and dynamics of RNA-protein complexes. Methods Enzymol., 339, 357-376.
    • (2001) Methods Enzymol. , vol.339 , pp. 357-376
    • Allen, M.1    Varani, L.2    Varani, G.3
  • 40
    • 16244394790 scopus 로고    scopus 로고
    • Structure determination of protein/RNA complexes by NMR
    • Wu, H.H., Finger, L.D. and Feigon, J. (2005) Structure determination of protein/RNA complexes by NMR. Methods Enzymol., 394, 525-545.
    • (2005) Methods Enzymol. , vol.394 , pp. 525-545
    • Wu, H.H.1    Finger, L.D.2    Feigon, J.3
  • 41
    • 24744460822 scopus 로고    scopus 로고
    • Extending the size of protein-RNA complexes studied by nuclear magnetic resonance spectroscopy
    • Mackereth, C.D., Simon, B. and Sattler, M. (2005) Extending the size of protein-RNA complexes studied by nuclear magnetic resonance spectroscopy. Chembiochem, 6, 1578-1582.
    • (2005) Chembiochem , vol.6 , pp. 1578-1582
    • Mackereth, C.D.1    Simon, B.2    Sattler, M.3
  • 42
  • 45
    • 0031941596 scopus 로고    scopus 로고
    • Preparation of uniformly isotope-labeled DNA oligonucleotides for NMR spectroscopy
    • Louis, J.M., Martin, R.G., Clore, G.M. and Gronenborn, A.M. (1998) Preparation of uniformly isotope-labeled DNA oligonucleotides for NMR spectroscopy. J. Biol. Chem., 273, 2374-2378.
    • (1998) J. Biol. Chem. , vol.273 , pp. 2374-2378
    • Louis, J.M.1    Martin, R.G.2    Clore, G.M.3    Gronenborn, A.M.4
  • 50
    • 0032573864 scopus 로고    scopus 로고
    • Enzymatic synthesis of region-specific isotope-labeled DNA oligomers for NMR analysis
    • Mer, G. and Chazin, W.J. (1998) Enzymatic synthesis of region-specific isotope-labeled DNA oligomers for NMR analysis. J. Am. Chem. Soc., 120, 607-608.
    • (1998) J. Am. Chem. Soc. , vol.120 , pp. 607-608
    • Mer, G.1    Chazin, W.J.2
  • 51
    • 0025012039 scopus 로고
    • DNA polymers of protein-binding sequences generated by PCR
    • Rudert, W.A. and Trucco, M. (1990) DNA polymers of protein-binding sequences generated by PCR. Nucleic Acids Res., 18, 6460-6460.
    • (1990) Nucleic Acids Res. , vol.18 , pp. 6460-6460
    • Rudert, W.A.1    Trucco, M.2
  • 52
    • 0026411008 scopus 로고
    • Concatemer chain-reaction-a Taq DNA Polymerase-mediated mechanism for generating long tandemly repetitive DNA-sequences
    • White, M.J., Fristensky, B.W. and Thompson, W.F. (1991) Concatemer chain-reaction-a Taq DNA Polymerase-mediated mechanism for generating long tandemly repetitive DNA-sequences. Anal. Biochem., 199, 184-190.
    • (1991) Anal. Biochem. , vol.199 , pp. 184-190
    • White, M.J.1    Fristensky, B.W.2    Thompson, W.F.3
  • 57
    • 44649084315 scopus 로고    scopus 로고
    • Preparation of multimilligram quantities of large, linear DNA molecules for structural studies
    • Muecke, M., Samuels, M., Davey, M. and Jeruzalmi, D. (2008) Preparation of multimilligram quantities of large, linear DNA molecules for structural studies. Structure, 16, 837-841.
    • (2008) Structure , vol.16 , pp. 837-841
    • Muecke, M.1    Samuels, M.2    Davey, M.3    Jeruzalmi, D.4
  • 58
    • 0001736975 scopus 로고
    • Conversion of mono- and oligodeoxyribonucleotides to 5'-triphosphates
    • Hoard, D.E. and Ott, D.G. (1965) Conversion of mono- and oligodeoxyribonucleotides to 5'-triphosphates. J. Am. Chem. Soc., 87, 1785-1788.
    • (1965) J. Am. Chem. Soc. , vol.87 , pp. 1785-1788
    • Hoard, D.E.1    Ott, D.G.2
  • 59
    • 2942683101 scopus 로고    scopus 로고
    • Global structure of a DNA three-way junction by solution NMR: towards prediction of 3H fold
    • Wu, B., Girard, F., van Buuren, B., Schleucher, J., Tessari, M. and Wijmenga, S. (2004) Global structure of a DNA three-way junction by solution NMR: towards prediction of 3H fold. Nucleic Acids Res., 32, 3228-3239.
    • (2004) Nucleic Acids Res. , vol.32 , pp. 3228-3239
    • Wu, B.1    Girard, F.2    van Buuren, B.3    Schleucher, J.4    Tessari, M.5    Wijmenga, S.6
  • 60
    • 25844524498 scopus 로고    scopus 로고
    • Slipped (CTG)-(CAG) repeats can be correctly repaired, escape repair or undergo error-prone repair
    • Panigrahi, G.B., Lau, R., Montgomery, S.E., Leonard, M.R. and Pearson, C.E. (2005) Slipped (CTG)-(CAG) repeats can be correctly repaired, escape repair or undergo error-prone repair. Nat. Struct. Mol. Biol., 12, 654-662.
    • (2005) Nat. Struct. Mol. Biol. , vol.12 , pp. 654-662
    • Panigrahi, G.B.1    Lau, R.2    Montgomery, S.E.3    Leonard, M.R.4    Pearson, C.E.5
  • 61
    • 0037133931 scopus 로고    scopus 로고
    • In vitro (CTG)-(CAG) expansions and deletions by human cell extracts
    • Panigrahi, G.B., Cleary, J.D. and Pearson, C.E. (2002) In vitro (CTG)-(CAG) expansions and deletions by human cell extracts. J. Biol. Chem., 277, 13926-13934.
    • (2002) J. Biol. Chem. , vol.277 , pp. 13926-13934
    • Panigrahi, G.B.1    Cleary, J.D.2    Pearson, C.E.3
  • 62
    • 23144440350 scopus 로고    scopus 로고
    • DINAMelt web server for nucleic acid melting prediction
    • Markham, N.R. and Zuker, M. (2005) DINAMelt web server for nucleic acid melting prediction. Nucleic Acids Res., 33, W577-W581.
    • (2005) Nucleic Acids Res. , vol.33
    • Markham, N.R.1    Zuker, M.2
  • 63
    • 0029836175 scopus 로고    scopus 로고
    • Preparation of specifically deuterated RNA for NMR studies using a combination of chemical and enzymatic synthesis
    • Tolbert, T.J. and Williamson, J.R. (1996) Preparation of specifically deuterated RNA for NMR studies using a combination of chemical and enzymatic synthesis. J. Am. Chem. Soc., 118, 7929-7940.
    • (1996) J. Am. Chem. Soc. , vol.118 , pp. 7929-7940
    • Tolbert, T.J.1    Williamson, J.R.2
  • 64
    • 0035874473 scopus 로고    scopus 로고
    • Inhibition of Escherichia coli CTP synthase by glutamate gamma-semialdehyde and the role of the allosteric effector GTP in glutamine hydrolysis
    • Bearne, S.L., Hekmat, O. and MacDonnell, J.E. (2001) Inhibition of Escherichia coli CTP synthase by glutamate gamma-semialdehyde and the role of the allosteric effector GTP in glutamine hydrolysis. Biochem. J., 356, 223-232.
    • (2001) Biochem. J. , vol.356 , pp. 223-232
    • Bearne, S.L.1    Hekmat, O.2    MacDonnell, J.E.3
  • 65
    • 0027292415 scopus 로고
    • Cloning, sequencing, and expression of the adenosylcobalamin-dependent ribonucleotide reductase from Lactobacillus leichmannii
    • Booker, S. and Stubbe, J. (1993) Cloning, sequencing, and expression of the adenosylcobalamin-dependent ribonucleotide reductase from Lactobacillus leichmannii. Proc. Natl Acad. Sci. USA, 90, 8352-8356.
    • (1993) Proc. Natl Acad. Sci. USA , vol.90 , pp. 8352-8356
    • Booker, S.1    Stubbe, J.2
  • 66
    • 0037151652 scopus 로고    scopus 로고
    • Determination of DNA structure in solution: enzymatic deuteration of the ribose 2' carbon
    • MacDonald, D. and Lu, P.Z. (2002) Determination of DNA structure in solution: enzymatic deuteration of the ribose 2' carbon. J. Am. Chem. Soc., 124, 9722-9723.
    • (2002) J. Am. Chem. Soc. , vol.124 , pp. 9722-9723
    • MacDonald, D.1    Lu, P.Z.2
  • 67
    • 0141539507 scopus 로고    scopus 로고
    • Cloning, expression, and purification of the His6-tagged hyper-thermostable dUTPase from Pyrococcus woesei in Escherichia coli: application in PCR
    • Dabrowski, S. and Ahring, B.K. (2003) Cloning, expression, and purification of the His6-tagged hyper-thermostable dUTPase from Pyrococcus woesei in Escherichia coli: application in PCR. Protein Expr. Purif., 31, 72-78.
    • (2003) Protein Expr. Purif. , vol.31 , pp. 72-78
    • Dabrowski, S.1    Ahring, B.K.2
  • 68
  • 69
    • 0842331877 scopus 로고    scopus 로고
    • Identification, cloning, and expression of bacteriophage T5 dnk gene encoding a broad specificity deoxyribonucleoside monophosphate kinase (EC 2.7.4.13)
    • Mikoulinskaia, G.V., Zimin, A.A., Feofanov, S.A. and Miroshnikov, A.I. (2004) Identification, cloning, and expression of bacteriophage T5 dnk gene encoding a broad specificity deoxyribonucleoside monophosphate kinase (EC 2.7.4.13). Protein Expr. Purif., 33, 166-175.
    • (2004) Protein Expr. Purif. , vol.33 , pp. 166-175
    • Mikoulinskaia, G.V.1    Zimin, A.A.2    Feofanov, S.A.3    Miroshnikov, A.I.4
  • 70
    • 0032190674 scopus 로고    scopus 로고
    • Cloning and expression in Escherichia coli of the recombinant His-tagged DNA polymerases from Pyrococcus furiosus and Pyrococcus woesei
    • Dabrowski, S. and Kur, J. (1998) Cloning and expression in Escherichia coli of the recombinant His-tagged DNA polymerases from Pyrococcus furiosus and Pyrococcus woesei. Protein Expr. Purif., 14, 131 138.
    • (1998) Protein Expr. Purif. , vol.14 , pp. 131-138
    • Dabrowski, S.1    Kur, J.2


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