A novel approach for uniform 13C and 15N labeling of DNA for NMR studies

Biochemical and Biophysical Research Communications

Volumn 290, Issue 3, 2002, Pages 928-932

  Ramanathan, Sunita ^a   Chary, K V R ^a   Rao, Basuthkar J ^a  

^a TATA INSTITUTE OF FUNDAMENTAL RESEARCH   (India)

Author keywords

Biosynthesis; Cloning; DNA; ESRA; HSQC; Isotope labeling; Minimal medium; NMR

Indexed keywords

AMMONIUM CHLORIDE; CARBON; CARBON 13; DNA; GLUCOSE DERIVATIVE; NITROGEN; NITROGEN 15; PLASMID VECTOR; RESTRICTION ENDONUCLEASE;

ANALYTIC METHOD; ARTICLE; BACTERIAL CELL; CARBON NUCLEAR MAGNETIC RESONANCE; CARBON SOURCE; CULTURE MEDIUM; DNA DETERMINATION; DNA PURIFICATION; DNA SEQUENCE; DNA TEMPLATE; ESCHERICHIA COLI; GENE AMPLIFICATION; METHODOLOGY; MOLECULAR CLONING; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; POLYACRYLAMIDE GEL ELECTROPHORESIS; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SYNTHESIS; TANDEM REPEAT;

ESCHERICHIA COLI;

EID: 0036290646     PISSN: 0006291X     EISSN: None     Source Type: Journal    
DOI: 10.1006/bbrc.2001.6306     Document Type: Article

References (14)

1. NMR spectroscopy: A multifaceted approach to macromolecular structure
2. New developments in isotope labeling strategies for protein solution NMR spectroscopy
3. Methodological advances in protein NMR
4. Multidimensional nuclear magnetic resonance methods for protein studies
5. NMR methodology for the study of nucleic acids
6. −15N-labeled DNA oligonucleotides
7. 15N-labeled DNA for NMR studies
8. 15N-labeled DNA for heteronuclear NMR studies
9. Enzymatic synthesis of region-specific isotopic-labeled DNA oligomers for NMR analysis
10. 15N labeling of long DNA oligomers
11. Preparation of uniformly isotope-labeled DNA oligonucleotides for NMR spectroscopy
12. Pure absorption gradient enhanced heteronuclear single quantum correlation spectroscopy with improved sensitivity
13. Preparation of isotopically labeled ribonucleotides for multidimensional NMR spectroscopy of RNA