Cloning and expression in Escherichia coli of the recombinant His-tagged DNA polymerases from Pyrococcus furiosus and Pyrococcus woesei

Protein Expression and Purification

Volumn 14, Issue 1, 1998, Pages 131-138

  Dabrowski, Slawomir ^a   Kur, Józef ^a  

^a GDANSK UNIVERSITY OF TECHNOLOGY   (Poland)

Author keywords

DNA polymerase; DNA sequence; Metal affinity chromatography; PCR; Recombinant protein

Indexed keywords

AFFINITY CHROMATOGRAPHY; AMINO ACID; BACTERIAL GENETICS; CLONING; DNA FRAGMENT; DNA ISOLATION; EXPRESSION VECTOR; GENE EXPRESSION; HIS TAGGED DNA POLYMERASE; NUCLEOTIDE SEQUENCE; POLYHISTIDINE TAG; POLYMERASE CHAIN REACTION; RECOMBINANT PLASMID; SEPHAROSE; STRUCTURAL GENE; THERMOSTABILITY;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; PROKARYOTA; PYROCOCCUS FURIOSUS; PYROCOCCUS WOESEI;

EID: 0032190674     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1006/prep.1998.0945     Document Type: Article

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