Sequencing and characterization of asclepain f: The first cysteine peptidase cDNA cloned and expressed from Asclepias fruticosa latex

Planta

Volumn 230, Issue 2, 2009, Pages 319-328

  Trejo, Sebastián A ^a,b   López, Laura M I ^a   Caffini, Néstor O ^a   Natalucci, Claudia L ^a   Canals, Francesc ^b,c   Avilés, Francesc X ^b  

^a UNIVERSIDAD NACIONAL DE LA PLATA   (Argentina)

^b UNIVERSITAT AUTÒNOMA DE BARCELONA   (Spain)

^c HOSPITAL UNIVERSITARI VALL D HEBRON   (Spain)

Author keywords

Asclepias fruticosa; Cloning; Cysteine endopeptidase; Gomphocarpus fruticosus subsp. fruticosus; Overexpression in Pichia pastoris; Plant latex

Indexed keywords

ASCLEPAIN; COMPLEMENTARY DNA; CYSTEINE PROTEINASE; VEGETABLE PROTEIN;

AMINO ACID SEQUENCE; ARTICLE; ASCLEPIAS; CHEMICAL STRUCTURE; CHEMISTRY; ENZYMOLOGY; GENETICS; MASS SPECTROMETRY; METABOLISM; MOLECULAR CLONING; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; PICHIA; PROTEIN TERTIARY STRUCTURE; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SEQUENCE ALIGNMENT;

AMINO ACID SEQUENCE; ASCLEPIAS; BASE SEQUENCE; CLONING, MOLECULAR; CYSTEINE ENDOPEPTIDASES; DNA, COMPLEMENTARY; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; PICHIA; PLANT PROTEINS; PROTEIN STRUCTURE, TERTIARY; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; SEQUENCE ALIGNMENT; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION;

ASCLEPIAS; ASCLEPIAS FRUTICOSA; GOMPHOCARPUS FRUTICOSUS; PICHIA PASTORIS;

EID: 67649470538     PISSN: 00320935     EISSN: 14322048     Source Type: Journal    
DOI: 10.1007/s00425-009-0942-2     Document Type: Article

References (34)

1. SF Altschul TL Madden AA Schaffer J Zhang Z Zhang W Miller DJ Lipman 1997 Gapped BLAST and PSI-BLAST: a new generation of protein database search programs Nucleic Acids Res 25 3389 3402 (Pubitemid 27359211)
2. EN Baker EJ Dodson 1980 Crystallographic refinement of the structure of actinidin at 1.7 Angstroms resolution by fast Fourier least-squares Acta Crystallogr A 36 559 572
3. AJ Barrett ND Rawlings 2001 Evolutionary lines of cysteine peptidases Biol Chem 382 727 733 (Pubitemid 32631709)
4. Barrett AJ, Rawlings ND (2004) Introduction: the clans and families of cysteine peptidases. In: Barrett AJ, Rawlings ND, Woessner JF (eds) Handbook of proteolytic enzymes, 2nd edn. Elsevier, London, pp 1051-1071
5. JU Bowie R Lüthy D Eisenberg 1991 A method to identify protein sequences that fold into a known three-dimensional structure Science 253 164 170 (Pubitemid 21917131)
6. MB Bradford 1976 A rapid and sensitive method for the quantitation of micrograms quantities of protein utilizing the principle of protein-dye binding Anal Biochem 72 248 254
7. D Brömme FS Nallaseth B Turk 2004 Production and activation of recombinant papain-like cysteine proteases Methods 32 199 206 (Pubitemid 38091627)
8. KH Choi RA Laursen KN Allen 1999 The 2.1 Angstrom structure of a cysteine protease with proline specificity from Ginger Rhizome, Zingiber officinale Biochemistry 38 11624 11633
9. LW Cohen VM Coghlan LC Dihel 1986 Cloning and sequencing of papain-encoding cDNA Gene 48 219 227
10. A Domsalla MF Melzig 2008 Occurrence and properties of proteases in plant lattices Planta Med 74 699 711 (Pubitemid 351880991)
11. J Drenth KH Kalk HM Swen 1976 Binding of chloromethyl ketone substrate analogues to crystalline papain Biochemistry 15 3731 3738
12. A El Moussaoui M Nijs C Paul R Wintjens J Vincentelli M Azarkan Y van Looze 2001 Revisiting the enzymes stored in the laticifers of Carica papaya in the context of their possible participation in the plant defence mechanism Cell Mol Life Sci 58 556 570 (Pubitemid 32382257)
13. IY Filippova EN Lysogorskaya ES Oksenoit GN Rudenskaya VM Stepanov 1984 l-Pyroglutamyl-l-phenylalanyl-l-leucine-p-nitroanilide-a chromogenic substrate for thiol proteinase assay Anal Biochem 143 293 297 (Pubitemid 15161195)
14. N Galtier M Gouy C Gautier 1996 SEAVIEW and PHYLO-WIN: two graphic tools for sequence alignment and molecular phylogeny Comput Appl Biosci 12 543 548
15. KM Karrer SL Pfeifer ME Di Tomas 1993 Two distinct gene subfamily within the family of cysteine protease genes Proc Natl Acad Sci USA 90 3063 3067
16. UK Laemmli 1970 Cleavage of structural proteins during the assembly of the head of bacteriophage T4 Nature 227 680 685
17. RA Laskowski MW MacArthur DS Moss JM Thornton 1993 PROCHECK: a program to check the stereochemical quality of protein structures J Appl Cryst 26 283 291
18. R Lüthy JU Bowie D Eisenberg 1992 Assessment of protein models with three-dimensional profiles Nature 356 83 85
19. DJ Maes F Bouckaert WL Poortmans Y Looze 1996 Structure of chymopapain at 1.7 A resolution Biochemistry 35 16292 16298
20. K Mizuguchi CM Deane TL Blundell MS Johnson JP Overington 1998 JOY: protein sequence-structure representation and analysis Bioinformatics 14 617 623 (Pubitemid 28461583)
21. H Nielsen J Engelbrecht S Brunak G von Heijne 1997 Identification of prokaryotic and eukaryotic signal peptides and prediction of their cleavage sites Protein Eng 10 1 6
22. Núñez MR, Jiye SM, Mizuguchi K (2002) Protein fold recognition and comparative modeling using HOMSTRAD, JOY and FUGUE. In: Tsigelny I (ed) Protein structure prediction: bioinformatic approach. International University Line, La Jolla, pp 143-169
23. RW Pickersgill P Rizkallah GW Harris PW Goodenough 1991 Determination of the structure of papaya protease omega Acta Crystallogr B 47 766 771
24. RW Pickersgill GW Harris E Garman 1992 Structure of monoclinic papain at 1.60 Angstroms resolution Acta Crystallogr B 48 59 67
25. ND Rawlings FR Morton CY Kok J Kong AJ Barrett 2008 MEROPS: the peptidase database Nucleic Acids Res 36 D320 D325
26. A Sali TL Blundell 1990 Definition of general topological equivalence in protein structures. A procedure involving comparison of properties and relationships through simulated annealing and dynamic programming J Mol Biol 212 403 428 (Pubitemid 20130737)
27. A Sali TL Blundell 1993 Comparative protein modelling by satisfaction of spatial restraints J Mol Biol 234 779 815
28. Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning a laboratory manual, 2nd edn. Cold Spring Harbor Laboratory Press, New York
29. MA Taylor KC Baker GS Briggs IF Connerton NJ Cummings KA Pratt DF Revell RB Freedman PW Goodenough 1995 Recombinant pro-regions from papain and papaya proteinase IV-are selective high affinity inhibitors of the mature papaya enzymes Protein Eng 8 59 62
30. JD Thompson DG Higgins TJ Gibson 1994 CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice Nucleic Acids Res 22 4673 4680 (Pubitemid 24354800)
31. SA Trejo LMI López CV Cimino NO Caffini CL Natalucci 2001 Purification and characterization of a new plant endopeptidase isolated from latex of Asclepias fruticosa L. (Asclepiadaceae) J Protein Chem 20 445 453
32. B Turk V Turk D Turk 1997 Structural and functional aspects of papain-like cysteine proteinases and their protein inhibitors Biol Chem 378 141 150 (Pubitemid 27230596)
33. RA Van der Hoorn JD Jones 2004 The plant proteolytic machinery and its role in defence Curr Opin Plant Biol 7 400 407 (Pubitemid 38871613)
34. B Wiederanders 2003 Structure-function relationships in class CA1 cysteine peptidase propeptides Acta Biochim Polon 50 691 713 (Pubitemid 38623802)