Purification and biochemical characterization of asclepain c I from the latex of Asclepias curassavica L.

Protein Journal

Volumn 23, Issue 6, 2004, Pages 403-411

  Liggieri, Constanza ^a   Arribére, M Cecilia ^a   Trejo, Sebastián A ^a   Canals, Francesc ^b   Avilés, Francesc X ^b   Priolo, Nora S ^a  

^a UNIVERSIDAD NACIONAL DE LA PLATA   (Argentina)

^b UNIVERSITAT AUTÒNOMA DE BARCELONA   (Spain)

Author keywords

Asclepiadaceae; Asclepias curassavica; Cysteine proteinases; Latex; Laticifers

Indexed keywords

ASCLEPAIN C I; CASEIN; CYSTEINE; EDETIC ACID; ESTER; GLUTAMINE DERIVATIVE; LATEX; PROTEINASE; UNCLASSIFIED DRUG; ASCLEPAIN; AZOCASEIN; CATION; CYSTEINE PROTEINASE; ION;

ALKALINITY; AMINO TERMINAL SEQUENCE; ARTICLE; ASCLEPIAS; CHEMICAL ANALYSIS; INCUBATION TIME; ION EXCHANGE CHROMATOGRAPHY; KINETICS; MASS SPECTROMETRY; MEASUREMENT; MOLECULAR WEIGHT; NONHUMAN; PH; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN DEGRADATION; PURIFICATION; TEMPERATURE MEASUREMENT; THERMOSTABILITY; ULTRACENTRIFUGATION; AGAR GEL ELECTROPHORESIS; AMINO ACID SEQUENCE; BIOCHEMISTRY; CHEMISTRY; DOSE RESPONSE; HEAT; ISOLATION AND PURIFICATION; METABOLISM; METHODOLOGY; MOLECULAR GENETICS; PROTEIN TERTIARY STRUCTURE; TIME;

APOCYNACEAE; ASCLEPIAS CURASSAVICA;

AMINO ACID SEQUENCE; ASCLEPIAS; BIOCHEMISTRY; CASEINS; CATIONS; CHROMATOGRAPHY, ION EXCHANGE; CYSTEINE; CYSTEINE ENDOPEPTIDASES; DOSE-RESPONSE RELATIONSHIP, DRUG; EDETIC ACID; ELECTROPHORESIS, AGAR GEL; ELECTROPHORESIS, POLYACRYLAMIDE GEL; HEAT; HYDROGEN-ION CONCENTRATION; IONS; KINETICS; LATEX; MASS SPECTROMETRY; MOLECULAR SEQUENCE DATA; PROTEIN STRUCTURE, TERTIARY; TIME FACTORS; ULTRACENTRIFUGATION;

EID: 16644385670     PISSN: 15723887     EISSN: 18758355     Source Type: Journal    
DOI: 10.1023/B:JOPC.0000039554.18157.69     Document Type: Article

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