Denaturant-dependent conformational changes in a β-trefoil protein: Global and residue-specific aspects of an equilibrium denaturation process

Biochemistry

Volumn 48, Issue 46, 2009, Pages 10934-10947

  Latypov, Ramil F ^a   Liu, Dingjiang ^b   Jacob, Jaby ^a   Harvey, Timothy S ^b   Bondarenko, Pavel V ^b   Kleemann, Gerd R ^a   Brems, David N ^b   Raibekas, Andrei A ^b  

^a AMGEN INC   (United States)

^b AMGEN INC   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CHEMICAL SHIFT ANALYSIS; CONFORMATIONAL CHANGE; CONFORMATIONAL FLEXIBILITY; CONFORMATIONAL PROPERTIES; DENATURANT CONCENTRATION; DENATURATION PROCESS; DENATURING CONDITIONS; ELEVATED TEMPERATURE; FOLDED STATE; GUANIDINE HYDROCHLORIDE; HIGH RESOLUTION; HSQC NMR; HUMAN INTERLEUKIN-1 RECEPTOR ANTAGONIST; IRREVERSIBLE AGGREGATION; LIMITED PROTEOLYSIS; MOLTEN GLOBULE; NATIVE CONFORMATION; NONUNIFORM; OPTICAL SPECTROSCOPY; PEAK INTENSITY; RADIUS OF GYRATION; SMALL ANGLE X-RAY SCATTERING; STRUCTURAL PERTURBATION; STRUCTURAL TRANSITIONS; TERTIARY STRUCTURES; TRANSITION REGIONS; TREFOIL PROTEINS; TWO-DIMENSIONAL NUCLEAR MAGNETIC RESONANCE;

AMINO ACIDS; CONCENTRATION (PROCESS); METABOLISM; NUCLEAR MAGNETIC RESONANCE; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; PROTEINS; RESONANCE; UREA; X RAY SCATTERING;

CONFORMATIONS;

INTERLEUKIN 1 RECEPTOR BLOCKING AGENT; TREFOIL PEPTIDE; UREA;

ARTICLE; CONFORMATIONAL TRANSITION; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; PRIORITY JOURNAL; PROTEIN CONFORMATION; PROTEIN DEGRADATION; PROTEIN DENATURATION; PROTEIN FOLDING; PROTEIN TERTIARY STRUCTURE; STRUCTURE ANALYSIS; X RAY CRYSTALLOGRAPHY;

CIRCULAR DICHROISM; ENDOPEPTIDASE K; FLUORESCENCE POLARIZATION; GUANIDINE; HUMANS; INTERLEUKIN 1 RECEPTOR ANTAGONIST PROTEIN; MODELS, MOLECULAR; NUCLEAR MAGNETIC RESONANCE, BIOMOLECULAR; PROTEIN CONFORMATION; PROTEIN DENATURATION; RECOMBINANT PROTEINS; SCATTERING, RADIATION; SPECTROMETRY, FLUORESCENCE; THERMODYNAMICS; UREA; X-RAYS;

LOTUS;

EID: 72749127303     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi901570k     Document Type: Article

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