Engineering of a novel hybrid enzyme: An anti-inflammatory drug target with triple catalytic activities directly converting arachidonic acid into the inflammatory prostaglandin E2

Protein Engineering, Design and Selection

Volumn 22, Issue 12, 2009, Pages 733-740

  Ruan, Ke He ^a   Cervantes, Vanessa ^a   So, Shui Ping ^a  

^a UNIVERSITY OF HOUSTON   (United States)

Author keywords

Cyclooxygenase (COX); Inflammation; Prostaglandin E2 (PGE 2); Prostaglandin E2 synthase (PGES); Protein engineering

Indexed keywords

ANIMAL MODEL; ANTI-INFLAMMATORIES; ANTI-INFLAMMATORY DRUGS; ARACHIDONIC ACIDS; C-TERMINUS; CATALYTIC ACTIVITY; CATALYTIC FUNCTIONS; CELLULAR LEVELS; COUPLING REACTION; CYCLOOXYGENASES; ENZYME ENGINEERING; FACE ORIENTATION; GAMMA-LINOLENIC ACID; HEK293 CELLS; HYBRID ENZYMES; INDUCIBLE ENZYME; INFLAMMATORY MEDIATORS; ISOFORMS; MEMBRANE TOPOLOGY; MICROSOMAL PROSTAGLANDIN; MPGES-1; NATIVE CONFORMATION; PATHOLOGICAL PROCESS; PROSTAGLANDINE; PROTEIN ENGINEERING; STRUCTURE-BASED; SYNTHASES; TRANS-MEMBRANE DOMAINS; TRANSMEMBRANES;

ACIDS; ACTIVATION ANALYSIS; BIOCHEMICAL ENGINEERING; CATALYSTS; CHEMICAL REACTIONS; ENZYME INHIBITION; GENETIC ENGINEERING; MODELS; PATHOLOGY; TOPOLOGY;

ENZYMES;

ANTIINFLAMMATORY AGENT; ARACHIDONIC ACID; CYCLOOXYGENASE 2; NONSTEROID ANTIINFLAMMATORY AGENT; PROSTAGLANDIN E2; PROSTAGLANDIN G; PROSTAGLANDIN G2;

ARTICLE; CATALYSIS; CELL STRAIN HEK293; CONTROLLED STUDY; CROSS COUPLING REACTION; DRUG SCREENING; ENDOPLASMIC RETICULUM; ENZYME ACTIVITY; ENZYME ENGINEERING; ENZYME STRUCTURE; HUMAN; HUMAN CELL; MEMBRANE STRUCTURE; PRIORITY JOURNAL;

ANTI-INFLAMMATORY AGENTS; ARACHIDONIC ACID; BIOCATALYSIS; CELL LINE; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CYCLOOXYGENASE 2; DINOPROSTONE; ELECTROPHORESIS, POLYACRYLAMIDE GEL; HUMANS; IMMUNOENZYME TECHNIQUES; INFLAMMATION; INTRAMOLECULAR OXIDOREDUCTASES; KINETICS; PROTEIN ENGINEERING;

ANIMALIA;

EID: 72649100254     PISSN: 17410126     EISSN: 17410134     Source Type: Journal    
DOI: 10.1093/protein/gzp058     Document Type: Article

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