An active triple-catalytic hybrid enzyme engineered by linking cyclo-oxygenase isoform-1 to prostacyclin synthase that can constantly biosynthesize prostacyclin, the vascular protector

FEBS Journal

Volumn 275, Issue 23, 2008, Pages 5820-5829

  Ruan, Ke He ^a   So, Shui Ping ^a   Cervantes, Vanessa ^a   Wu, Hanjing ^a,b   Wijaya, Cori ^a   Jentzen, Rebecca R ^a,b  

^a UNIVERSITY OF HOUSTON   (United States)

^b UNIVERSITY OF TEXAS   (United States)

Author keywords

COX; Cyclo oxygenase; PG12; Prostacyclin; Prostaglandin 12

Indexed keywords

CYCLOOXYGENASE 2 INHIBITOR; ISOENZYME; PROSTACYCLIN SYNTHASE; PROSTAGLANDIN SYNTHASE;

ARTICLE; BIOSYNTHESIS; CARBOXY TERMINAL SEQUENCE; CATALYSIS; CELL STRAIN HEK293; EMBRYO; ENZYME ACTIVITY; ENZYME ENGINEERING; ENZYME SYNTHESIS; HUMAN; HUMAN CELL; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN FUNCTION; THROMBOCYTE AGGLUTINATION;

6-KETOPROSTAGLANDIN F1 ALPHA; ARACHIDONIC ACID; CATALYSIS; CELL LINE; CLONING, MOLECULAR; CYCLOOXYGENASE 1; CYTOCHROME P-450 ENZYME SYSTEM; ENDOPLASMIC RETICULUM; EPOPROSTENOL; GENE EXPRESSION; HUMANS; INTRAMOLECULAR OXIDOREDUCTASES; KINETICS; MODELS, MOLECULAR; PLATELET AGGREGATION; PROTEIN ENGINEERING; RECOMBINANT FUSION PROTEINS; THROMBOXANE B2;

EID: 55949086582     PISSN: 1742464X     EISSN: 17424658     Source Type: Journal    
DOI: 10.1111/j.1742-4658.2008.06703.x     Document Type: Article

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