Occludin gene expression as an early in vitro sign for mild eye irritation assessment

Toxicology in Vitro

Volumn 24, Issue 1, 2010, Pages 276-285

  Meloni, Marisa ^a   Pauly, Aude ^b   Servi, Barbara De ^a   Varlet, Béatrice Le ^a,c   Baudouin, Christophe ^b  

^a VitroScreen   (Italy)

^b CENTRE HOSPITALIER NATIONAL D OPHTALMOLOGIE DES QUINZE VINGTS   (France)

^c Links Ingénierie   (France)

Author keywords

BAK; Eye compatibility; Eye irritation; HCE; IL 8; MTT test; Occludin gene expression; Tear substitutes

Indexed keywords

ABAK; ARTIFICIAL TEAR; BENZALKONIUM CHLORIDE; COMOD; INTERLEUKIN 8; IRRITANT AGENT; OCCLUDIN; OXYD; PERBORATE; THIOMERSAL; UNCLASSIFIED DRUG;

ARTICLE; BIOLOGICAL MODEL; CELL VIABILITY; CONTROLLED STUDY; CORNEA EPITHELIUM; CYTOKINE RELEASE; EYE IRRITATION; GENE EXPRESSION; IN VITRO STUDY; TOXICITY TESTING;

CELL SURVIVAL; COLORING AGENTS; DRUG EVALUATION, PRECLINICAL; ENZYME-LINKED IMMUNOSORBENT ASSAY; EYE DISEASES; FLUORESCENT ANTIBODY TECHNIQUE; GENE EXPRESSION; GENETIC MARKERS; HUMANS; INTERLEUKIN-8; IRRITANTS; MEMBRANE PROTEINS; OPHTHALMIC SOLUTIONS; REPRODUCIBILITY OF RESULTS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, MESSENGER; TETRAZOLIUM SALTS; THIAZOLES;

ANIMALIA;

EID: 72049130547     PISSN: 08872333     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.tiv.2009.08.016     Document Type: Article

References (38)

1. Ajani G., Sato N., Mack J.A., and Maytin E.V. Cellular responses to disruption of the permeability barrier in a three-dimensional organotypic epidermal model. Experimental Cell Research 313 (2007) 3005-3015
2. Balls M., Botham P.A., Brunner L.H., and Spielmann H. The EC/HO international validation study on alternatives to Draize eye irritation test. Toxicology In Vitro 9 (1995) 871-929
3. Ban Y., Cooper L.J., Fullwood N.J., Nakamura T., Tsuzuki M., Koizumi N., Dota A., Mochida C., and Kinoshita S. Comparison of ultrastructure, tight junction-related protein expression and barrier function of human corneal epithelial cells cultivated on amniotic membrane with and without air-lifting. Experimental Eye Research 76 (2003) 735-743
4. Baudouin C., Hamard P., Liang H., Creuzot-Garcher C., Bensoussan L., and Brignole F. Conjunctival epithelial cell expression of interleukins and inflammatory markers in glaucoma patients treated over the long term. Ophthalmology 111 12 (2004) 2186-2192
5. Baudouin C., Liang H., Bremond-Gignac D., Hamard P., Hreiche R., Creuzot-Garcher C., Warnet J.M., and Brignole-Baudouin F. CCR 4 and CCR 5 expression in conjunctival specimens as differential markers of T(H)1/T(H)2 in ocular surface disorders. Journal of Allergy and Clinical Immunology 116 3 (2005) 614-619
6. Becker U., Ehrhardt C., Schneider M., Muys L., Gross D., Eschmann K., Schaefer U.F., and Lehr C.M. A comparative evaluation of corneal epithelial cell cultures for assessing ocular permeability. Alternatives to Laboratory Animals 36 (2008) 33-44
7. Boverhof D.R., and Zacharewsky T.R. Toxicogenomics in risk assessment: applications and needs. Toxicological Sciences 89 2 (2006) 352-360
8. Chuang E.Y., Li D.Q., Bian F., Zheng X., and Pflugfelder S.C. Effects of contact lens multipurpose solutions on human cornea epithelial survival and barrier function. Eye & Contact Lens 34 5 (2008) 281-286
9. Coquette A., Berna N., Vandenbosch A., Rosdy M., De Wever B., and Poumay Y. Analysis of interleukin-1 and interleukin-8 expression and release in in vitro reconstructed human epidermis for the prediction of in vivo skin irritation and/or sensitization. Toxicology In Vitro 17 (2003) 311-321
10. Daston G.P. Genomics and development of risk assessment. Birth Defects Research Part A Clinical and Molecular Teratology 79 (2007) 1-7
11. De Saint Jean M., Brignole F., Bringuier A.F., Bauchet A., Feldmann G., and Baudouin C. Effects of benzalkonium chloride on growth and survival of change conjunctival cells. Investigative Ophthalmology & Visual Science 40 (1999) 619-630
12. Debbasch C., Ebenhahn C., Dami N., Pericoi M., Van den Berghe C., Cottin M., and Nohynek G.J. Eye irritation of low irritant cosmetic formulations: correlation of in vitro results with clinical data and product composition. Food & Chemical Toxicology 43 (2005) 155-165
13. Doucet O., Lanvin M., and Zastrow L. Comparison on three in vitro methods for the assessment of the eye irritating potential of formulated cosmetic products. In Vitro and Molecular Toxicology 12 (1999) 63-76
14. Doucet O., Lanvin M., Thillou C., Linossier C., Pupat C., Merlin B., and Zastrow L. Reconstituted human corneal epithelium: a new alternative to the Draize eye test for the assessment of the eye irritation potential of chemicals and cosmetic products. Toxicology In Vitro 20 4 (2006) 499-512
15. Draize J.H., Woodard G., and Calvery H.O. Methods for study of irritation and toxicity of substances applied topically to the skin and mucous membranes. The Journal of Pharmacology and Experimental Therapeutics 82 (1944) 377-390
16. ESAC, 2007. ESAC Statement on the conclusions of the ICCVAM retrospective study on organotypic in vitro assays as screening tests to identify potential ocular corrosives and severe irritants as determined by US EPA, EU (R41) and UN GHS classifications in a tiered testing strategy, as part of a weight of evidence approach. Website: Joint Research Center (accessed 28.09.07).
17. Eskes, C., Bessou, S., Bruner, L., Curren, R., Harbell, J., Jones, P., Kreiling, R., Liebsch, M., McNamee, P., Pape, W., Prinsen, M., Seidle, T., Vanparys, P., Worth, A. & Zuang, V., 2005. Eye irritation. In: Eskes C., Zuang V. (Eds.), Alternative (Non-Animal) Methods for Cosmetics Testing: Current Status and Future Prospects. Alternatives to Laboratory Animals 33 (1), 47-81 (Subchapter 3.3).
18. Frentz M., Goss M., Reim M., and Schrage N.F. Repeated exposure to benzalkonium chloride in the ex vivo eye irritation test (EVEIT): observation of isolated corneal damage and healing. Alternatives to Laboratory Animals 36 (2008) 25-32
19. Guenoun J.M., Baudouin C., Rat P., Pauly A., Warnet J.M., and Brignole-Baudouin F. In vitro comparison of cytoprotective and antioxidative effects of latanoprost, travoprost, and bimatoprost on conjunctiva-derived epithelial cells. Investigative Ophthalmology & Visual Science 46 (2005) 4594-4599
20. Guenoun J.M., Baudouin C., Rat P., Pauly A., Warnet J.M., and Brignole-Baudouin F. In vitro study of inflammatory potential and toxicity profile of latanoprost, travoprost, and bimatoprost in conjunctiva-derived epithelial cells. Investigative Ophthalmology & Visual Science 46 (2005) 2444-2450
21. Kimura K., Teranishi S., and Nishida T. Interleukin-1beta-induced disruption of barrier function in cultured human corneal epithelial cells. Investigative Ophthalmology & Visual Science 50 2 (2009) 597-603
22. Matter K., and Balda M.S. Epithelial tight junctions, gene expression and nucleo-junctional interplay. Journal of Cell Science 120 Pt. 9 (2007) 1505-1511
23. McCarthy K.M., Skare I.B., Stankewich M.C., Furuse M., Tsukita S., Rogers R.A., Lynch R.D., and Schneeberger E.E. Occludin is a functional component of the tight junction. Journal of Cell Science 109 Pt. 9 (1996) 2287-2298
24. Meloni M., Dalla Valle P., Cappadoro M., and De Wever B. The importance of multiple endpoint analysis (MEA) using reconstituted human tissue models for irritation and biocompatibility assay. Invitox Congress Proceedings 4 (2002) 7
25. Mitjans M., Viviani B., Lucchi L., Galli C.L., Marinovich M., and Corsini E. Role of p38 MAPK in the selective release of IL-8 induced by chemical allergen in naïve THP1 cells. Toxicology In Vitro 22 (2008) 386-395
26. Mondino B.J., Salamon S.M., and Zaidman G.W. Allergic and toxic reactions of soft contact lens wearers. Survey of Ophthalmology 26 6 (1982) 337-344
27. Nguyen D.H., Beuerman R.W., De Wever B., and Rosdy M. Three-dimensional construct of the human corneal epithelium for in vitro toxicology. In: Salem H., and Katz S.A. (Eds). Alternatives Toxicological Methods (2003), CRC Press 147-159
28. Pauly A., Brignole-Baudouin F., Labbé A., Liang H., Warnet J.M., and Baudouin C. New tools for the evaluation of toxic ocular surface changes in the rat. Investigative Ophthalmology & Visual Science 48 (2007) 5473-5483
29. Pauly A., Meloni M., Brignole-Baudouin F., Warnet J.M., and Baudouin C. Multiple endpoint analysis of the 3D-reconstituted corneal epithelium after treatment with benzalkonium chloride: early detection of toxic damage. Investigative Ophthalmology & Visual Science 50 4 (2009) 1644-1652
30. Pisella P.J., Fillacier K., Elena P.P., Debbasch C., and Baudouin C. Comparison of the effects of preserved and unpreserved formulations of timolol on the ocular surface of albino rabbits. Ophthalmic Research 32 (2000) 3-8
31. Powers, M.J., Amenuvor, L., Rodin, R., Darner, J., 2007. An in vitro Human Corneal Epithelial Model for Evaluation of Ocular Response to Xenobiotic Compounds. Investigative Ophthalmology & Visual Science 48, 790 (E-Abstract).
32. Reichl S. Cell culture models of the human cornea - a comparative evaluation of their usefulness to determine ocular drug absorption in vitro. Journal of Pharmacy and Pharmacology 60 (2008) 299-307
33. SCCP/111/07. European commission directorate general health & consumer protection: memorandum on actual status of alternative method on the use of experimental animals in the safety assessment of cosmetic ingredients in the European Union. Opinion adopted at 12th plenary session, 19 June, 2007.
34. Schneeberger, E.E., Lynch, R.D., 2004. The tight junction: a multifunctional complex. American Journal of Physiology-Cell Physiology 286, C1213-C1228.
35. Sosnová-Netuková M., Kuchynka P., and Forrester J.V. The suprabasal layer of cornea epithelial cells represents the major barrier site to the passive movement of small molecules and trafficking leukocytes. British Journal of Ophthalmology 91 (2007) 372-378
36. Tosti A., and Tosti G. Thimerosal: a hidden allergen in ophthalmology. Contact Dermatitis 18 5 (1988) 268-273
37. Van Goethem F., Adriaens E., Alépée N., Straube F., De Wever B., Cappadoro M., Catoire S., Hansen E., Wolf A., and Vanparys P. Prevalidation of a new in vitro reconstituted human cornea model to assess the eye irritating potential of chemicals. Toxicology In Vitro 20 (2006) 1-17
38. Weil C.S., and Scala R.A. Study of intra- and interlaboratory variability in the results of rabbit eye and skin irritation tests. Toxicology and Applied Pharmacology 19 (1971) 276-360