Chapter 19 Hemoglobin-Vesicles as an Artificial Oxygen Carrier

Methods in Enzymology

Volumn 465, Issue C, 2009, Pages 363-384

  Sakai, Hiromi ^a   Sou, Keitaro ^a   Tsuchida, Eishun ^a  

^a WASEDA UNIVERSITY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIBODY; HEMOGLOBIN; HEMOGLOBIN ANTIBODY; HEMOGLOBIN DERIVATIVE; HEMOGLOBIN VESICLE; LIPOSOME; LIPOSOME ENCAPSULATED HEMOGLOBIN; OXYGEN; UNCLASSIFIED DRUG;

ARTIFICIAL OXYGEN CARRIER; BINDING AFFINITY; BINDING SITE; BLOOD; BLOOD COMPATIBILITY; BLOOD OXYGENATION; BLOOD RHEOLOGY; BLOOD TRANSFUSION; CIRCULATION; DRUG CLEARANCE; DRUG STABILITY; ENZYMATIC DEGRADATION; ERYTHROCYTE; HUMAN; IMMUNOHISTOCHEMISTRY; ION PAIR CHROMATOGRAPHY; LIPID VESICLE; LIPOSOMAL DELIVERY; LIQUID; MOLECULAR STABILITY; NANOENCAPSULATION; NONHUMAN; OSMOSIS; OSMOTIC PRESSURE; OXYGEN TRANSPORT; PHYSICAL CHEMISTRY; PRIORITY JOURNAL; PROTEIN PURIFICATION; REGULATORY MECHANISM; RETICULOENDOTHELIAL SYSTEM; REVIEW; SURFACE PROPERTY; SUSPENSION; ULTRAFILTRATION; ANIMAL; ARTICLE; CATTLE; CHEMISTRY; ISOLATION AND PURIFICATION;

ANIMALS; CATTLE; HEMOGLOBINS; HUMANS; LIPOSOMES; OXYGEN;

EID: 71849095830     PISSN: 00766879     EISSN: None     Source Type: Book Series    
DOI: 10.1016/S0076-6879(09)65019-9     Document Type: Review

References (108)

1. Abe H., Ikebuchi K., Hirayama J., Fujihara M., Takeoka S., Sakai H., Tsuchida E., and Ikeda H. Virus inactivation in hemoglobin solution by heat treatment. Artif. Cells Blood Substit. Immobil. Biotechnol. 29 (2001) 381-388
2. Abe H., Fujihara M., Azuma H., Ikeda H., Ikebuchi K., Takeoka S., Tsuchida E., and Harashima H. Interaction of hemoglobin vesicles, a cellular-type artificial oxygen carrier, with human plasma: Effects on coagulation, kallikrein-kinin, and complement systems. Artif. Cells Blood Substit. Immobil. Biotechnol. 34 (2006) 1-10
3. Abe H., Azuma H., Yamaguchi M., Fujihara M., Ikeda H., Sakai H., Takeoka S., and Tsuchida E. Effects of hemoglobin vesicles, a liposomal artificial oxygen carrier, on hematological responses, complement and anaphylactic reactions in rats. Artif. Cells Blood Substit. Immobil. Biotechnol. 35 (2007) 157-172
4. Akama K., Awai K., Yano Y., Tokuyama S., and Nakano Y. In vitro and in vivo stability of polymerized mixed liposomes coposed of 2, 4-octadecadienoyl groups of phospholipids. Polym. Adv. Technol. 11 (2000) 280-287
5. Atoji T., Aihara M., Sakai H., Tsuchida E., and Takeoka S. Hemoglobin vesicles containing methemoglobin and L-tyrosine to suppress methemoglobin formation in vitro and in vivo. Bioconjug. Chem. 17 (2006) 1241-1245
6. Bangham A.D., and Horne R.W. Negative staining of phospholipids and their structure modification by surface-active agents as observed in the electron microscope. J. Mol. Biol. 8 (1964) 660-668
7. Beissinger R.L., Farmer M.C., and Gossage J.L. Liposome-encapsulated hemoglobin as a red cell surrogate. ASAIO Trans. 32 (1986) 58-63
8. Cabrales P., Sakai H., Tsai A.G., Takeoka S., Tsuchida E., and Intaglietta M. Oxygen transport by low and normal oxygen affinity hemoglobin vesicles in extreme hemodilution. Am. J. Physiol. Heart Circ. Physiol. 288 (2005) H1885-H1892
9. Chang T.M.S. Blood substitutes based on modified hemoglobin prepared by encapsulation or crosslinking: An overview. Biomater. Artif. Cells Immobilization Biotechnol. 20 (1991) 159-182
10. Chang T.M. Hemoglobin-based red blood cell substitutes. Artif. Organs 28 (2004) 789-794
11. Chonn A., Cullis P.R., and Devine D.V. The role of surface charge in the activation of the classical and alternative pathways of complement by liposomes. J. Immunol. 146 (1991) 4234-4241
12. Chung J.E., Hamada K., Sakai H., Takeoka S., Nishide H., and Tsuchida E. Ligand exchange reaction of carbonylhemoglobin to oxyhemoglobin in a hemoglobin liquid membrane. Nippon Kagaku Kaishi 1995 (1995) 123-127
13. 2 affinity. Am. J. Physiol. Heart Circ. Physiol. 285 (2003) H1140-H1147
14. Contaldo C., Plock J., Sakai H., Takeoka S., Tsuchida E., Leunig M., Banic A., and Erni D. New generation of hemoglobin-based oxygen carriers evaluated for oxygenation of critically ischemic hamster flap tissue. Crit. Care Med. 33 (2005) 806-812
15. DeLoach J.R., Sheffield C.L., and Spates G.E. A continuous-flow high yield process for preparation of lipid-free hemoglobin. Anal. Biochem. 157 (1986) 191-198
16. Djordjevich L., and Ivankovich A.D. Liposomes as carriers of hemoglobin. In: Gregoriadis G. (Ed). Liposomes as Drug Carriers (1988), John Wiley & Sons 551-567 Chapter 39
17. Djordjevich L., and Miller I.F. Lipid encapsulated hemoglobin as a synthetic erythrocyte. Fed. Proc. 36 (1977) 567
18. Farmer M.C., and Gaber B.P. Liposome-encapsulated hemoglobin as an artificial oxygen carrying system. Methods Enzymol. 149 (1987) 184-200
19. Fukutomi I., Sakai H., Takeoka S., Nishide H., Tsuchida E., and Sakai K. Carbonylation of oxyhemoglobin solution using a membrane oxygenator. J. Artif. Organs 5 (2002) 102-107
20. Gaber B.P., Yager P., Sheridan J.P., and Chang E.L. Encapsulation of hemoglobin in phospholipid vesicles. FEBS Lett. 153 (1983) 285-288
21. Hamilton R.G., and Kickler T.S. Bovine hemoglobin (glutamer-250, Hemopure)-specific immunoglobulin G antibody cross-reacts with human hemoglobin but does not lyse red blood cells in vitro. Transfusion 47 (2007) 723-728
22. Hayward J.A., Levine D.M., Neufeld L., Simon S.R., Johnston D.S., and Chapman D. Polymerized liposomes as stable oxygen-carriers. FEBS Lett. 187 (1985) 261-266
23. Hosoi F., Omichi H., Akama K., Awai K., Endo S., and Nakano Y. Radiation-induced polymerization of unsaturated phospholipid mixtures for the synthesis of artificial red cells. Nucl. Instrum. Methods Phys. Res. B 131 (1997) 329-334
24. Huang Y., Takeoka S., Sakai H., Abe H., Hirayama J., Ikebuchi K., Ikeda H., and Tsuchida E. Complete deoxygenation from a hemoglobin solution by an electrochemical method and heat treatment for virus inactivation. Biotechnol. Prog. 18 (2002) 101-107
25. Hunt C.A., Burnette R.R., MacGregor R.D., Strubbe A.E., Lau D.T., Taylor N., and Kawada H. Synthesis and evaluation of prototypal artificial red cells. Science 230 (1985) 1165-1168
26. Ito T., Fujihara M., Abe H., Yamaguchi M., Wakamoto S., Takeoka S., Sakai H., Tsuchida E., Ikeda H., and Ikebuchi K. Effects of poly(ethyleneglycol)-modified hemoglobin vesicles on N-formyl-methionyl-leucyl-phenylalanine-induced responses of polymorphonuclear neutrophils in vitro. Artif. Cells Blood Substit. Immobil. Biotechnol. 29 (2001) 427-437
27. Izumi Y., Sakai H., Takeoka S., Kose T., Hamada K., Yoshizu A., Horinouchi H., Kato R., Nishide H., Tsuchida E., and Kobayashi K. Evaluation of the capabilities of a hemoglobin vesicle as an artificial oxygen carrier in a rat exchange transfusion model. ASAIO J. 43 (1997) 289-297
28. Jopski B., Pirkl V., Jaroni H.-W., Schubert R., and Schmidt K.-H. Preparation of hemoglobin-containing liposomes using octyl glucoside and octyltetraoxyethylene. Biochim. Biophys. Acta 978 (1989) 79-84
29. Kato A., Arakawa M., and Kondo T. Liposome-type artificial red blood cells stabilized with carboxymethylchitin. Nippon Kagaku Kaishi 6 (1984) 987-991 (in Japanese)
30. Kerwin B.A., Akers M.J., Apostol I., Moore-Einsel C., Etter J.E., Hess E., Lippincott J., Levine J., Mathews A.J., Revilla-Sharp P., Schubert R., and Looker D.L. Acute and long-term stability studies of deoxy hemoglobin and characterization of ascorbate-induced modifications. J. Pharm. Sci. 88 (1999) 79-88
31. Klibanov A.L., Maruyama K., Torchilin V.P., and Huang L. Amphipathic polyethylene glycols effectively prolong the circulation time of liposomes. FEBS Lett. 268 (1990) 235-237
32. Li S., Nickels J., and Palmer A.F. Liposome-encapsulated actin-hemoglobin (LEAcHb) artificial blood substitutes. Biomaterials 26 (2005) 3759-3769
33. Liu L., and Yonetani T. Preparation and characterization of liposome-encapsulated haemoglobin by a freeze-thaw method. J. Microencapsul. 11 (1994) 409-421
34. Loughrey H.C., Bally M.B., Reinish L.W., and Cullis P.R. The binding of phosphatidylglycerol liposomes to rat platelets is mediated by complement. Thromb. Haemost. 64 (1990) 172-176
35. Martini J., Cabrales P., Tsai A.G., and Intaglietta M. Mechanotransduction and the homeostatic significance of maintaining blood viscosity in hypotension, hypertension and haemorrhage. J. Intern. Med. 259 (2006) 364-372
36. Meyuhas D., Nir S., and Lichtenberg D. Aggregation of phospholipid vesicles by water-soluble polymers. Biophys. J. 71 (1996) 2602-2612
37. Mobed M., and Chang T.M.S. Preparation and surface characterization of carboxymethylchitin-incorporated submicron bilayer-lipid membrane artificial cells (liposomes) encapsulating hemoglobin. Biomater. Artif. Cells Immobilization Biotechnol. 19 (1991) 731-744
38. Murray J.A., Ledlow A., Launspach J., Evans D., Loveday M., and Conklin J.L. The effects of recombinant human hemoglobin on esophageal motor function in humans. Gastroenterology 109 (1995) 1241-1248
39. Naito Y., Fukutomi I., Masada Y., Sakai H., Takeoka S., Tsuchida E., Abe H., Hirayama J., Ikebuchi K., and Ikeda H. Virus removal from hemoglobin solution using Planova membrane. J. Artif. Organs 5 (2002) 141-145
40. Natanson C., Kern S.J., Lurie P., Banks S.M., and Wolfe S.M. Cell-free hemoglobin-based blood substitutes and risk of myocardial infarction and death: A meta-analysis. JAMA 299 (2008) 2304-2312
41. Ogata Y., Goto H., Kimura T., and Fukui H. Development of neo red cells (NRC) with the enzymatic reduction system of methemoglobin. Artif. Cells Blood Substit. Immobil. Biotechnol. 25 (1997) 417-427
42. Palmer A.F., Sun G., and Harris D.R. Tangential flow filtration of hemoglobin. Biotechnol. Prog. 25 (2009) 189-199
43. Papahadjopoulos D., Allen T.M., Gabizon A., Mayhew E., Matthay K., Huang S.K., Lee K.D., Woodle M.C., Lasic D.D., Redemann C., and Martin F.J. Sterically stabilized liposomes: Improvements in pharmacokinetics and antitumor therapeutic efficacy. Proc. Natl. Acad. Sci. USA 88 (1991) 11460-11464
44. Pape A., Kertscho H., Meier J., Horn O., Laout M., Steche M., Lossen M., Theisen A., Zwissler B., and Habler O. Improved short-term survival with polyethylene glycol modified hemoglobin liposomes in critical normovolemic anemia. Intensive Care Med. 34 (2008) 1534-1543
45. Phillips W.T., Klipper R., Fresne D., Rudolph A.S., Javors M., and Goins B. Platelet reactivity with liposome-encapsulated hemoglobin in the rat. Exp. Hematol. 25 (1997) 1347-1356
46. Phillips W.T., Klipper R.W., Awasthi V.D., Rudolph A.S., Cliff R., Kwasiborski V., and Goins B.A. Polyethylene glycol-modified liposome-encapsulated hemoglobin: A long circulating red cell substitute. J. Pharmacol. Exp. Ther. 288 (1999) 665-670
47. Plock J.A., Contaldo C., Sakai H., Tsuchida E., Leunig M., Banic A., Menger M.D., and Erni D. Is the Hb in Hb vesicles infused for isovolemic hemodilution necessary to improve oxygenation in critically ischemic hamster skin?. Am. J. Physiol. Heart Circ. Physiol. 289 (2005) H2624-H2631
48. Plock J.A., Tromp A.E., Contaldo C., Spanholtz T., Sinovcic D., Sakai H., Tsuchida E., Leunig M., Banic A., and Erni D. Hemoglobin vesicles reduce hypoxia-related inflammation in critically ischemic hamster flap tissue. Crit. Care Med. 35 (2007) 899-905
49. Rabinovici R., Rudolph A.S., Ligler F.S., Smith III E.F., and Feuerstein G. Biological responses to exchange transfusion with liposome-encapsulated hemoglobin. Circ. Shock 37 (1992) 124-133
50. Rabinovici R., Rudolph A.S., Vernick J., and Feuerstein G. A new salutary resuscitative fluid: Liposome encapsulated hemoglobin/hypertonic saline solution. J. Trauma 35 (1993) 121-127
51. Rudolph A.S. The freeze-dried preservation of liposome encapsulated hemoglobin: A potential blood substitute. Cryobiology 25 (1988) 277-284
52. 99mTc-labeled liposome-encapsulated hemoglobin in an anesthetized rabbit. Proc. Natl Acad. Sci. USA 88 (1991) 10976-10980
53. Sakai H., and Tsuchida E. Hemoglobin-vesicles for a transfusion alternative and targeted oxygen delivery. J. Liposome Res. 17 (2007) 227-235
54. Sakai H., Takeoka S., Yokohama H., Nishide H., and Tsuchida E. Encapsulation of Hb into unsaturated lipid vesicles and γ-ray polymerization. Polym. Adv. Technol. 3 (1992) 389-394
55. Sakai H., Takeoka S., Yokohama H., Seino Y., Nishide H., and Tsuchida E. Purification of concentrated Hb using organic solvent and heat treatment. Protein Expr. Purif. 4 (1993) 563-569
56. Sakai H., Hamada K., Takeoka S., Nishide H., and Tsuchida E. Physical properties of hemoglobin vesicles as red cell substitutes. Biotechnol. Prog. 12 (1996) 119-125
57. Sakai H., Takeoka S., Park S.I., Kose T., Izumi Y., Yoshizu A., Nishide H., Kobayashi K., and Tsuchida E. Surface-modification of hemoglobin vesicles with poly(ethylene glycol) and effects on aggregation, viscosity, and blood flow during 90%-exchange transfusion in anesthetized rats. Bioconjug. Chem. 8 (1997) 23-30
58. Sakai H., Tsai A.G., Kerger H., Park S.I., Takeoka S., Nishide H., Tsuchida E., and Intaglietta M. Subcutaneous microvascular responses to hemodilution with red cell substitutes consisting of polyethylene glycol-modified vesicles encapsulating hemoglobin. J. Biomed. Mater. Res. 40 (1998) 66-78
59. 2 affinity. Am. J. Physiol. Heart Circ. Physiol. 276 (1999) H553-H562
60. Sakai H., Yuasa M., Onuma H., Takeoka S., and Tsuchida E. Synthesis and physicochemical characterization of a series of hemoglobin-based oxygen carriers: Objective comparison between cellular and acellular types. Bioconjug. Chem. 11 (2000) 56-64
61. Sakai H., Tomiyama K., Sou K., Takeoka S., and Tsuchida E. Poly(ethylene glycol)-conjugation and deoxygenation enable long-term preservation of hemoglobin-vesicles as oxygen carriers in a liquid state. Bioconjug. Chem. 11 (2000) 425-432
62. 2 carriers determine constriction of resistance arteries and hypertension. Am. J. Physiol. Heart Circ. Physiol. 279 (2000) H908-H915
63. Sakai H., Horinouchi H., Tomiyama K., Ikeda E., Takeoka S., Kobayashi K., and Tsuchida E. Hemoglobin-vesicles as oxygen carriers: Influence on phagocytic activity and histopathological changes in reticuloendothelial system. Am. J. Pathol. 159 (2001) 1079-1088
64. Sakai H., Takeoka S., Wettstein R., Tsai A.G., Intaglietta M., and Tsuchida E. Systemic and microvascular responses to the hemorrhagic shock and resuscitation with Hb-vesicles. Am. J. Physiol. Heart Circ. Physiol. 283 (2002) H1191-H1199
65. Sakai H., Masada Y., Takeoka S., and Tsuchida E. Characteristics of bovine hemoglobin as a potential source of hemoglobin-vesicles for an artificial oxygen carrier. J. Biochem. 131 (2002) 611-617
66. Sakai H., Horinouchi H., Masada Y., Yamamoto M., Takeoka S., Kobayashi K., and Tsuchida E. Hemoglobin-vesicles suspended in recombinant human serum albumin for resuscitation from hemorrhagic shock in anesthetized rats. Crit. Care Med. 32 (2004) 539-545
67. Sakai H., Masada Y., Horinouchi H., Ikeda H., Takeoka S., Suematsu M., Kobayashi K., and Tsuchida E. Physiologic capacity of reticuloendothelial system for degradation of hemoglobin-vesicles (artificial oxygen carriers) after massive intravenous doses by daily repeated infusions for 14 days. J. Pharmacol. Exp. Ther. 311 (2004) 874-884
68. 2-binding of concentrated hemoglobin solution coencapsulated in phospholipid vesicles. Bioconjug. Chem. 15 (2004) 1037-1045
69. Sakai H., Hisamoto S., Fukutomi I., Sou K., Takeoka S., and Tsuchida E. Detection of lipopolysaccharide in hemoglobin-vesicles by Limulus amebocyte lysate test with kinetic-turbidimetric gel clotting analysis and pretreatment of surfactant. J. Pharm. Sci. 93 (2004) 310-321
70. Sakai H., Sato A., Takeoka S., and Tsuchida E. Rheological property of hemoglobin vesicles (artificial oxygen carriers) suspended in a series of plasma substitute aqueous solutions. Langmuir 23 (2007) 8121-8128
71. Sakai H., Sato A., Masuda K., Takeoka S., and Tsuchida E. Encapsulation of concentrated hemoglobin solution in phospholipid vesicles retards the reaction with NO, but not CO, by intracellular diffusion barrier. J. Biol. Chem. 283 (2008) 1508-1517
72. Sakai H., Sato A., Sobolewski P., Takeoka S., Frangos J.A., Kobayashi K., Intaglietta M., and Tsuchida E. NO and CO binding profiles of hemoglobin vesicles as artificial oxygen carriers. Biochim. Biophys. Acta 1784 (2008) 1441-1447
73. Sakai H., Seishi Y., Obata Y., Takeoka S., Horinouichi H., Tsuchida E., and Kobayashi K. Fluid resuscitation with artificial oxygen carriers in hemorrhaged rats: Profiles of hemoglobin-vesicle degradation and hematopoiesis for 14 days. Shock 31 (2009) 192-200
74. Sakai H., Okamoto M., Ikeda E., Horinouchi H., Kobayashi K., and Tsuchida E. Histopathological changes of rat brain after direct injection of hemoglobin vesicles (oxygen carriers) and neurological impact in an intracerebral hemorrhage model. J. Biomed. Mater. Res. A 88A (2009) 34-42
75. Sakai H., Horinouchi H., Tsuchida E., and Kobayashi K. Hemoglobin-vesicles and red blood cells as carriers of carbon monoxide prior to oxygen for resuscitation after hemorrhagic shock in a rat model. Shock 31 (2009) 507-514
76. Sakai H., Sato A., Takeoka S., and Tsuchida E. Mechanism of flocculate formation of phospholipid vesicles suspended in a series of water-soluble biopolymers. Biomacromolecules 10 (2009) 2344-2350
77. Sakai H., Sato A., Okuda N., Takeoka S., Maeda N., and Tsuchida E. Peculiar flow patterns of RBCs suspended in viscous fluids and perfused through a narrow tube (25 μm). Am. J. Physiol. Heart Circ. Physiol. 297 (2009) H905-H910
78. Sato T., Kobayashi K., Sekiguchi S., and Tsuchida E. Characteristics of artificial red cells: Hemoglobin-encapsulated in poly-lipid vesicles. ASAIO J. 38 (1992) M580-M584
79. Sato T., Sakai H., Sou K., Medebach M., Glatter O., and Tsuchida E. Static structure and dynamics of hemoglobin vesicle (HbV) developed as a transfusion alternative. J. Phys. Chem. B 113 (2009) 8418-8428
80. Sheffield C.L., Spates G.E., Droleskey R.E., Green R., and DeLoach J.R. Preparation of lipid-free human hemoglobin by dialysis and ultrafiltration. Biotechnol. Appl. Biochem. 9 (1987) 230-238
81. Sou K., and Tsuchida E. Electrostatic interactions and complement activation on the surface of phospholipid vesicle containing acidic lipids: Effect of the structure of acidic groups. Biochim. Biophys. Acta 1778 (2008) 1035-1041
82. Sou K., Naito Y., Endo T., Takeoka S., and Tsuchida E. Effective encapsulation of proteins into size-controlled phospholipid vesicles using freeze-thawing and extrusion. Biotechnol. Prog. 19 (2003) 1547-1552
83. Sou K., Klipper R., Goins B., Tsuchida E., and Phillips W.T. Circulation kinetics and organ distribution of Hb vesicles developed as a red blood cell substitute. J. Pharmacol. Exp. Ther. 312 (2005) 702-709
84. Szebeni J., Breuer J.H., Szelenyi J.G., Bathori G., Lelkes G., and Hollan S.R. Oxidation and denaturation of hemoglobin encapsulated in liposomes. Biochim. Biophys. Acta 798 (1984) 60-67
85. Szebeni J., Di Iorio E.E., Hauser H., and Winterhalter K.H. Encapsulation of hemoglobin in phospholipid liposomes: Characterization and stability. Biochemistry 24 (1985) 2827-2832
86. Szebeni J., Fontana J.L., Wassef N.M., Mongan P.D., Morse D.S., Dobbins D.E., Stahl G.L., Bunger R., and Alving C.R. Hemodynamic changes induced by liposomes and liposome-encapsulated hemoglobin in pigs: A model for pseudoallergic cardiopulmonary reactions to liposomes. Role of complement and inhibition by soluble CR1 and anti-C5a antibody. Circulation 99 (1999) 2302-2309
87. Szebeni J., Baranyi L., Sávay S., Bodó M., Milosevits J., Alving C.R., and Bünger R. Complement activation-related cardiac anaphylaxis in pigs: Role of C5a anaphylatoxin and adenosine in liposome-induced abnormalities in ECG and heart function. Am. J. Physiol. Heart Circ. Physiol. 290 (2005) H1050-H1058
88. Taguchi K., Maruyama T., Iwao Y., Sakai H., Kobayashi K., Horinouchi H., Tsuchida E., Kai T., and Otagiri M. Pharmacokinetics of single and repeated injection of hemoglobin-vesicles in hemorrhagic shock rat model. J. Control. Release 136 (2009) 232-239
89. Taguchi K., Urata Y., Anraku M., Maruyama T., Watanabe H., Sakai H., Horinouchi H., Kobayashi K., Tsuchida E., Kai T., and Otagiri M. Pharmacokinetic study of enclosed hemoglobin and outer lipid component after the administration of hemoglobin-vesicles as an artificial oxygen carrier. Drug Dispos. Metabol. 37 (2009) 1456-1463
90. Takahashi A. Characterization of neo red cells (NRCs), their function and safety in vivo tests. Artif. Cells Blood Substit. Immobil. Biotechnol. 23 (1995) 347-354
91. Takeoka S., Sakai H., Nishide H., and Tsuchida E. Preparation conditions of human hemoglobin-vesicles covered with lipid membranes. Jpn. J. Artif. Organs 22 (1993) 566-569
92. Takeoka S., Terase K., Sakai H., Yokohama H., Nishide H., and Tsuchida E. Interaction between phosphoslipid assemblies and hemoglobin (Hb). J. Macromol. Sci. Pure Appl. Chem. A31 (1994) 97-108
93. Takeoka S., Sakai H., Terease K., Nishide H., and Tsuchida E. Characteristics of Hb-vesicles and encapsulation procedure. Artif. Cells Blood Substit. Immobil. Biotechnol. 22 (1994) 861-866
94. Takeoka S., Ohgushi T., Terase K., and Tsuchida E. Layer-controlled hemoglobin vesicles by interaction of hemoglobin with a phospholipid assembly. Langmuir 12 (1996) 1755-1759
95. Takeoka S., Sakai H., Kose T., Mano Y., Seino Y., Nishide H., and Tsuchida E. Methemoglobin formation in hemoglobin vesicles and reduction by encapsulated thiols. Bioconjug. Chem. 8 (1997) 539-544
96. 2 reaction and lipid peroxidation. Bioconjug. Chem. 13 (2002) 1302-1308
97. Teramura Y., Kanazawa H., Sakai H., Takeoka S., and Tsuchida E. The prolonged oxygen-carrying ability of Hb vesicles by coencapsulation of catalase in vivo. Bioconjug. Chem. 14 (2003) 1171-1176
98. Tsai A.G., Friesenecker B., McCarthy M., Sakai H., and Intaglietta M. Plasma viscosity regulates capillary perfusion during extreme hemodilution in hamster skinfold model. Am. J. Physiol. Heart Circ. Physiol. 275 (1998) H2170-H2180
99. Tsuchida E.Ed. Blood Substitutes: Present and Future Perspectives (1998), Elsevier, Chichester
100. Vandegriff K.D., Young M.A., Lohman J., Bellelli A., Samaja M., Malavalli A., and Winslow R.M. CO-MP4, a polyethylene glycol-conjugated haemoglobin derivative and carbon monoxide carrier that reduces myocardial infarct size in rats. Br. J. Pharmacol. 154 (2008) 1649-1661
101. Vidal-Naquet A., Gossage J.L., Sullivan T.P., Haynes J.W., Gilruth B.H., Beissinger R.L., Sehgal L.R., and Rosen A.L. Liposome-encapsulated hemoglobin as an artificial red blood cell: Characterization and scale-up. Biomater. Artif. Cells Artif. Organs 17 (1989) 531-552
102. Vivier A., Vuillemard J.C., Ackermann H.W., and Poncelet D. Large-scale blood substitute production using a microfluidizer. Biomater. Artif. Cells Immobilization Biotechnol. 20 (1992) 377-397
103. Wakamoto S., Fujihara M., Abe H., Sakai H., Takeoka S., Tsuchida E., Ikeda H., and Ikebuchi K. Effects of poly(ethyleneglycol)-modified hemoglobin vesicles on agonist-induced platelet aggregation and RANTES release in vitro. Artif. Cells Blood Substit. Immobil. Biotechnol. 29 (2001) 191-201
104. Wakamoto S., Fujihara M., Abe H., Yamaguchi M., Azuma H., Ikeda H., Takeoka S., and Tsuchida E. Effects of hemoglobin vesicles on resting and agonist-stimulated human platelets in vitro. Artif. Cells Blood Substit. Immobil. Biotechnol. 33 (2005) 101-111
105. Wang L., Morizawa K., Tokuyama S., Satoh T., and Tsuchida E. Modulation of oxygen-carrying capacity of artificial red cells (ARC). Polym. Adv. Technol. 4 (1992) 8-11
106. Winslow R., and Chapman K.W. Pilot-scale preparation of hemoglobin solutions. Methods Enzymol. 231 (1994) 3-16
107. Yamazaki M., Aeba R., Yozu R., and Kobayashi K. Use of hemoglobin vesicles during cardiopulmonary bypass priming prevents neurocognitive decline in rats. Circulation 114 1 Suppl (2006) I220-I225
108. Yoshioka H. Surface modification of haemoglobin-containing liposomes with poly(ethylene glycol) prevents liposome aggregation in blood plasma. Biomaterials 12 (1991) 861-864