Chapter 41 Parallel Methods for Expression and Purification

Methods in Enzymology

Volumn 463, Issue C, 2009, Pages 767-785

  Lesley, Scott A ^a  

^a GENOMICS INSTITUTE OF THE NOVARTIS RESEARCH FOUNDATION   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANALYTIC METHOD; GENE CONSTRUCT; MOLECULAR CLONING; MUTAGENESIS; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN PURIFICATION; PROTEIN TARGETING; REVIEW; ANIMAL; BIOLOGICAL MODEL; CELL FRACTIONATION; CHEMISTRY; GENE EXPRESSION; GENE TRANSFER; GENE VECTOR; GENETICS; HIGH THROUGHPUT SCREENING; HUMAN; ISOLATION AND PURIFICATION; METABOLISM; METHODOLOGY; PHYSIOLOGY;

RECOMBINANT PROTEIN;

ANIMALS; CELL FRACTIONATION; CLONING, MOLECULAR; GENE EXPRESSION; GENE TRANSFER TECHNIQUES; GENETIC VECTORS; HIGH-THROUGHPUT SCREENING ASSAYS; HUMANS; MICROCHEMISTRY; MODELS, BIOLOGICAL; RECOMBINANT PROTEINS;

EID: 71549120128     PISSN: 00766879     EISSN: None     Source Type: Book Series    
DOI: 10.1016/S0076-6879(09)63041-X     Document Type: Chapter

References (27)

1. Altschul S.F., Gish W., Miller W., Myers E.W., and Lipman D.J. Basic local alignment search tool. J. Mol. Biol. 215 (1990) 403-410
2. Aslanidis C., and de Jong P.J. Ligation-independent cloning of PCR products (LIC-PCR). Nucleic Acids Res. 18 (1990) 6069-6074
3. Consortium U. The universal protein resource (UniProt). Nucleic Acids Res. 36 (2008) D190-D195
4. Finn R.D., Tate J., Mistry J., Coggill P.C., Sammut S.J., Hotz H.R., Ceric G., Forslund K., Eddy S.R., Sonnhammer E.L., and Bateman A. The Pfam protein families database. Nucleic Acids Res. 36 (2008) D281-D288
5. Graslund S., Nordlund P., Weigelt J., Hallberg B.M., Bray J., Gileadi O., Knapp S., Oppermann U., Arrowsmith C., Hui R., Ming J., dhe-Paganon S., et al. Protein production and purification. Nat. Methods 5 (2008) 135-146
6. Graslund S., Sagemark J., Berglund H., Dahlgren L.G., Flores A., Hammarstrom M., Johansson I., Kotenyova T., Nilsson M., Nordlund P., and Weigelt J. The use of systematic N- and C-terminal deletions to promote production and structural studies of recombinant proteins. Protein Expr. Purif. 58 (2008) 210-221
7. Harrison J., Molloy P.L., and Clark S.J. Direct cloning of polymerase chain reaction products in an XcmI T-vector. Anal. Biochem. 216 (1994) 235-236
8. Hatley J.L., Temple G.F., and Brasch M.A. DNA cloning using in vivo site-specific recombination. Genome Res. 10 (2000) 1788-1795
9. Kanehisa M., Araki M., Goto S., Hattori M., Hirakawa M., Itoh M., Katayama T., Kawashima S., Okuda S., Tokimatsu T., and Yamanishi Y. KEGG for linking genomes to life and the environment. Nucleic Acids Res. 36 (2008) D480-D484
10. Kapust R.B., and Waugh D.S. Escherichia coli maltose-binding protein is uncommonly effective at promoting the solubility of polypeptides to which it is fused. Protein Sci. 8 (1999) 1668-1674
11. Karp P.D., Ouzounis C.A., Moore-Kochlacs C., Goldovsky L., Kaipa P., Ahren D., Tsoka S., Darzentas N., Kunin V., and Lopez-Bigas N. Expansion of the BioCyc collection of pathway/genome databases to 160 genomes. Nucleic Acids Res. 33 (2005) 6083-6089
12. Kim Y., Dementieva I., Zhou M., Wu R., Lezondra L., Quartey P., Joachimiak G., Korolev O., Li H., and Joachimiak A. Automation of protein purification for structural genomics. J. Struct. Funct. Genomics 5 (2004) 111-118
13. Klock H.E., Koesema E.J., Knuth M.W., and Lesley S.A. Combining the polymerase incomplete primer extension method for cloning and mutagenesis with microscreening to accelerate structural genomics efforts. Proteins 71 (2008) 982-994
14. Kost T.A., and Condreay J.P. Recombinant baculoviruses as mammalian cell gene-delivery vectors. Trends Biotechnol. 20 (2002) 173-180
15. Lesley S.A. High-throughput proteomics: Protein expression and purification in the postgenomic world. Protein Expr. Purif. 22 (2001) 159-164
16. Lesley S.A., Graziano J., Cho C.Y., Knuth M.W., and Klock H.E. Gene expression response to misfolded protein as a screen for soluble recombinant protein. Protein Eng. 15 (2002) 153-160
17. Lesley S.A., Kuhn P., Godzik A., Deacon A.M., Mathews I., Kreusch A., Spraggon G., Klock H.E., McMullan D., Shin T., Vincent J., Robb A., et al. Structural genomics of the Thermotoga maritima proteome implemented in a high-throughput structure determination pipeline. Proc. Natl. Acad. Sci. USA 99 (2002) 11664-11669
18. Liu Q., Li M.Z., Leibham D., Cortez D., and Elledge S.J. The univector plasmid-fusion system, a method for rapid construction of recombinant DNA without restriction enzymes. Curr. Biol. 8 (1998) 1300-1309
19. Marsden B.D., and Knapp S. Doing more than just the structure-structural genomics in kinase drug discovery. Curr. Opin. Chem. Biol. 12 (2008) 40-45
20. Martinez Molina D., Cornvik T., Eshaghi S., Haeggstrom J.Z., Nordlund P., and Sabet M.I. Engineering membrane protein overproduction in Escherichia coli. Protein Sci. 17 (2008) 673-680
21. McMullan D., Canaves J.M., Quijano K., Abdubek P., Nigoghossian E., Haugen J., Klock H.E., Vincent J., Hale J., Paulsen J., and Lesley S.A. High-throughput protein production for X-ray crystallography and use of size exclusion chromatography to validate or refute computational biological unit predictions. J. Struct. Funct. Genomics 6 (2005) 135-141
22. Pantoliano M.W., Petrella E.C., Kwasnoski J.D., Lobanove V.S., Myslik J., Graf E., Carver T., Asel E., Springer B.A., Lane P., and Salemme F.R. High-density miniaturized thermal shift assays as a general strategy for drug discovery. J. Biomol. Screen. 6 (2001) 429-440
23. Peng S., Sommerfelt M., Logan J., Huang Z., Jilling T., Kirk K., Hunter E., and Sorscher E. One-step affinity isolation of recombinant protein using the baculovirus/insect cell expression system. Protein Expr. Purif. 4 (1993) 95-100
24. Shuman S. Novel approach to molecular cloning and polynucleotide synthesis using vaccinia DNA topoisomerase. J. Biol. Chem. 269 (1994) 32678-32684
25. Studier F.W. Protein production by auto-induction in high density shaking cultures. Protein Expr. Purif. 41 (2005) 207-234
26. Tropea J.E., Cherry S., and Waugh D.S. Expression and purification of soluble His(6)-tagged TEV protease. Methods Mol. Biol. 498 (2009) 297-307
27. Waldo G.S., Standish B.M., Berendzen J., and Terwilliger T.C. Rapid protein-folding assay using green fluorescent protein. Nat. Biotechnol. 17 (1999) 691-695