High-recovery one-step purification of the DNA-binding protein Fur by mild guanidinium chloride treatment

Process Biochemistry

Volumn 45, Issue 2, 2010, Pages 292-296

  Pellicer, Silvia ^a,b   Bes, M Teresa ^a,b   González, Andrés ^a,b   Neira, José L ^b,c   Peleato, M Luisa ^a,b   Fillat, María F ^a,b  

^a Department of Biochemistry and Molecular and Cell Biology ^*  (Spain)

^b UNIVERSITY OF ZARAGOZA   (Spain)

^c MIGUEL HERNÁNDEZ UNIVERSITY   (Spain)

Author keywords

DNA binding proteins; Ferric uptake regulator; Guanidinium chloride; High yield recovery

Indexed keywords

CHAOTROPIC AGENTS; CRUDE EXTRACT; CYANOBACTERIUM ANABAENA; DEEP KNOWLEDGE; DNA-BINDING DOMAIN; DNA-BINDING PROTEIN; FERRIC UPTAKE REGULATOR; GENERAL APPLICATIONS; GUANIDINIUM CHLORIDES; HYDROPHOBIC INTERACTIONS; IMINODIACETATE; NMR SPECTROSCOPY; ONE-STEP PURIFICATION; PROTEIN-DNA INTERACTIONS; REGULATORY PROTEIN; STRUCTURAL DATA; TERTIARY STRUCTURES;

CHLORINE COMPOUNDS; CHROMATOGRAPHIC ANALYSIS; DNA; ESCHERICHIA COLI; GENE EXPRESSION; HYDROPHOBIC CHROMATOGRAPHY; HYDROPHOBICITY; KNOWLEDGE ENGINEERING; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; NUCLEIC ACIDS; OLIGOMERS; PURIFICATION; ZINC;

PROTEINS;

ANABAENA; ESCHERICHIA COLI; NOSTOC SP. PCC 7120;

EID: 71249129868     PISSN: 13595113     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.procbio.2009.09.019     Document Type: Article

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