The effect of environmental conditions on extracellular protease activity in controlled fermentations of Aspergillus niger

Microbiology

Volumn 155, Issue 10, 2009, Pages 3430-3439

  Braaksma, Machtelt ^a,b   Smilde, Age K ^a,b   van der Werf, Mariët J ^a,b   Punt, Peter J ^a,b  

^a TNO   (Netherlands)

^b KLUYVER CENTRE FOR GENOMICS OF INDUSTRIAL FERMENTATION   (Netherlands)

Author keywords

[No Author keywords available]

Indexed keywords

AMMONIA; CITRIC ACID; GLUCOSE; LACTOSE; NITROGEN; PROLINE; PROTEINASE; SUCROSE; XYLOSE;

ARTICLE; ASPERGILLUS NIGER; CARBON SOURCE; ENVIRONMENTAL FACTOR; ENZYME ACTIVITY; FERMENTATION OPTIMIZATION; FUNGUS CULTURE; NITROGEN CONCENTRATION; NONHUMAN; PH; PHENOTYPE; PRIORITY JOURNAL; PROTEIN EXPRESSION;

ASPERGILLUS NIGER; CULTURE MEDIA; FERMENTATION; FUNGAL PROTEINS; HYDROGEN-ION CONCENTRATION; PEPTIDE HYDROLASES;

ASPERGILLUS NIGER; FUNGI;

EID: 71049123227     PISSN: 13500872     EISSN: None     Source Type: Journal    
DOI: 10.1099/mic.0.031062-0     Document Type: Article

References (44)

1. Bennett, J. W. & Lasure, L. L. (1991). Growth media. In More Gene Manipulations in Fungi, pp. 441-447. Edited by J. W. Bennett & L. L. Lasure. San Diego, CA: Academic Press.
2. Berka, R. M., Ward, M., Wilson, L. J., Hayenga, K. J., Kodama, K. H., Carlomagno, L. P. & Thompson, S. A. (1990). Molecular cloning and deletion of the gene encoding aspergillopepsin A from Aspergillus awamori. Gene 86, 153-162.
3. Bos, C. J., Debets, A. J. M., Swart, K., Huybers, A., Kobus, G. & Slakhorst, S. M. (1988). Genetic analysis and the construction of master strains for assignment of genes to six linkage groups in Aspergillus niger. Curr Genet 14, 437-443.
4. Braaksma, M. & Punt, P. J. (2008). Aspergillus as a cell factory for protein production: controlling protease activity in fungal production. In The Aspergilli: Genomics, Medical Aspects, Biotechnology, and Research Methods, pp. 441-455. Edited by G. H. Goldman & S. A. Osmani. Boca Raton, FL: CRC Press.
5. Buxton, F. P., Gwynne, D. I. & Davies, R. W. (1985). Transformation of Aspergillus niger using the argB gene of Aspergillus nidulans. Gene 37, 207-214.
6. Cohen, B. L. (1972). Ammonium repression of extracellular protease in Aspergillus nidulans. J Gen Microbiol 71, 293-299.
7. Cohen, B. L. (1981). Regulation of protease production in Aspergillus. Trans Br Mycol Soc 76, 447-450.
8. de Vries, R. P., Burgers, K., van de Vondervoort, P. J., Frisvad, J. C., Samson, R. A. & Visser, J. (2004). A new black Aspergillus species, A. vadensis, is a promising host for homologous and heterologous protein production. Appl Environ Microbiol 70, 3954-3959.
9. Eilers, P. H. C. (2003). A perfect smoother. Anal Chem 75, 3631-3636.
10. Heerikhuisen, M., van den Hondel, C. A. M. J. J. & Punt, P. J. (2005). Aspergillus sojae. In Production of Recombinant Proteins. Novel Microbial and Eucaryotic Expression Systems, pp. 191-214. Edited by G. Gelissen. Weinheim: Wiley.
11. Holm, K. A. (1980). Automated colorimetric determination of acid proteinase activity in fermentation samples using a trinitrobenzenesulphonic acid reagent. Analyst 105, 18-24.
12. Iimura, Y., Gomi, K., Uzu, H. & Hara, S. (1987). Transformation of Aspergillus oryzae through plasmid-mediated complementation of the methionine-auxotrophic mutation. Agric Biol Chem 51, 323-328.
13. Jarai, G. & Buxton, F. (1994). Nitrogen, carbon, and pH regulation of extracellular acidic proteases of Aspergillus niger. Curr Genet 26, 238-244.
14. Katz, M. E., Flynn, P. K., vanKuyk, P. A. & Cheetham, B. F. (1996). Mutations affecting extracellular protease production in the filamentous fungus Aspergillus nidulans. Mol Gen Genet 250, 715-724.
15. Katz, M. E., Bernardo, S. M. & Cheetham, B. F. (2008). The interaction of induction, repression and starvation in the regulation of extracellular proteases in Aspergillus nidulans: Evidence for a role for CreA in the response to carbon starvation. Curr Genet 54, 47-55.
16. Kelly, J. M. & Hynes, M. J. (1985). Transformation of Aspergillus niger by the amdS gene of Aspergillus nidulans. EMBO J 4, 475-479.
17. Kennedy, M. & Krouse, D. (1999). Strategies for improving fermentation medium performance: a review. J Ind Microbiol Biotechnol 23, 456-475.
18. Li, Q., Harvey, L. M. & McNeil, B. (2008). The effects of bioprocess parameters on extracellular proteases in a recombinant Aspergillus niger B1-D. Appl Microbiol Biotechnol 78, 333-341.
19. Lin, Y., Means, G. E. & Feeney, R. E. (1969). The action of proteolytic enzymes on N,N-dimethyl proteins. Basis for a microassay for proteolytic enzymes. J Biol Chem 244, 789-793.
20. Liu, F., Li, W., Ridgway, D., Gu, T. & Moo-Young, M. (1998). Inhibition of extracellular protease secretion by Aspergillus niger using cell immobilization. Biotechnol Lett 20, 539-542.
21. Lundstedt, T., Seifert, E., Abramo, L., Thelin, B., Nyström, Å., Pettersen, J. & Bergman, R. (1998). Experimental design and optimization. Chemom Intell Lab Syst 42, 3-40.
22. Mattern, I. E., van Noort, J. M., van den Berg, P., Archer, D. B., Roberts, I. N. & van den Hondel, C. A. (1992). Isolation and characterization of mutants of Aspergillus niger deficient in extracellular proteases. Mol Gen Genet 234, 332-336.
23. Moralejo, F. J., Cardoza, R. E., Gutiérrez, S., Sisniega, H., Faus, I. & Martín, J. F. (2000). Overexpression and lack of degradation of thaumatin in an aspergillopepsin A-defective mutant of Aspergillus awamori containing an insertion in the pepA gene. Appl Microbiol Biotechnol 54, 772-777.
24. Moralejo, F. J., Cardoza, R. E., Gutiérrez, S., Lombraňa, M., Fierro, F. & Martín, J. F. (2002). Silencing of the aspergillopepsin B (pepB) gene of Aspergillus awamori by antisense RNA expression or protease removal by gene disruption results in a large increase in thaumatin production. Appl Environ Microbiol 68, 3550-3559.
25. O'Donnell, D., Wang, L., Xu, J., Ridgway, D., Gu, T. & Moo-Young, M. (2001). Enhanced heterologous protein production in Aspergillus niger through pH control of extracellular protease activity. Biochem Eng J 8, 187-193.
26. Papagianni, M. & Moo-Young, M. (2002). Protease secretion in glucoamylase producer Aspergillus niger cultures: fungal morphology and inoculum effects. Process Biochem 37, 1271-1278.
27. Papagianni, M., Joshi, N. & Moo-Young, M. (2002). Comparative studies on extracellular protease secretion and glucoamylase production by free and immobilized Aspergillus niger cultures. J Ind Microbiol Biotechnol 29, 259-263.
28. Pel, H. J., de Winde, J. H., Archer, D. B., Dyer, P. S., Hofmann, G., Schaap, P. J., Turner, G., de Vries, R. P., Albang, R. & other authors (2007). Genome sequencing and analysis of the versatile cell factory Aspergillus niger CBS 513.88. Nat Biotechnol 25, 221-231.
29. Punt, P. J., Schuren, F. H. J., Lehmbeck, J., Christensen, T., Hjort, C. & van den Hondel, C. A. M. J. J. (2008). Characterization of the Aspergillus niger prtT, a unique regulator of extracellular protease encoding genes. Fungal Genet Biol 45, 1591-1599.
30. Sumner, J. B. & Somers, G. F. (1949). Dinitrosalicylic method for glucose. In Laboratory Experiments in Biological Chemistry, pp. 38-39. Edited by J. B. Sumner & G. F. Somers. New York: Academic Press.
31. Unkles, S. E., Campbell, E. I., de Ruiter-Jacobs, Y. M. J. T., Broekhuijsen, M., Macro, J. A., Carrez, D., Contreras, R., van den Hondel, C. A. M. J. J. & Kinghorn, J. R. (1989). The development of a homologous transformation system for Aspergillus oryzae based on the nitrate assimilation pathway: a convenient and general selection system for filamentous fungal transformation. Mol Gen Genet 218, 99-104.
32. van den Hombergh, J. P. T. W., Jarai, G., Buxton, F. P. & Visser, J. (1994). Cloning, characterization and expression of pepF, a gene encoding a serine carboxypeptidase from Aspergillus niger. Gene 151, 73-79.
33. van den Hombergh, J. P. T. W., van de Vondervoort, P. J. I., van der Heijden, N. C. B. A. & Visser, J. (1995). New protease mutants in Aspergillus niger result in strongly reduced in vitro degradation of target proteins; genetical and biochemical characterization of seven complementation groups. Curr Genet 28, 299-308.
34. van den Hombergh, J. P. T. W., Sollewijn-Gelpke, M. D., van de Vondervoort, P. J. I., Buxton, F. P. & Visser, J. (1997a). Disruption of three acid proteases in Aspergillus niger: effects on protease spectrum, intracellular proteolysis, and degradation of target proteins. Eur J Biochem 247, 605-613.
35. van den Hombergh, J. P., van de Vondervoort, P. J., Fraissinet-Tachet, L. & Visser, J. (1997b). Aspergillus as a host for heterologous protein production: the problem of proteases. Trends Biotechnol 15, 256-263.
36. van Hartingsveldt, W., Mattern, I. E., van Zeijl, C. M. J., Pouwels, P. H. & van den Hondel, C. A. M. J. J. (1987). Development of a homologous transformation system for Aspergillus niger based on the pyrG gene. Mol Gen Genet 206, 71-75.
37. van Noort, J. M., van den Berg, P. & Mattern, I. E. (1991). Visualization of proteases within a complex sample following their selective retention on immobilized bacitracin, a peptide antibiotic. Anal Biochem 198, 385-390.
38. Wang, Y., Xue, W., Sims, A. H., Zhao, C., Wang, A., Tang, G., Qin, J. & Wang, H. (2008). Isolation of four pepsin-like protease genes from Aspergillus niger and analysis of the effect of disruptions on heterologous laccase expression. Fungal Genet Biol 45, 17-27.
39. Weatherburn, M. W. (1967). Phenol-hypochlorite reaction for determination of ammonia. Anal Chem 39, 971-974.
40. Wiebe, M. G. (2003). Stable production of recombinant proteins in filamentous fungi - problems and improvements. Mycologist 17, 140-144.
41. Wiebe, M. G., Karandikar, A., Robson, G. D., Trinci, A. P. J., Candia, J.-L. F., Trappe, S., Wallis, G., Rinas, U., Derkx, P. M. F. & other authors (2001). Production of tissue plasminogen activator (t-PA) in Aspergillus niger. Biotechnol Bioeng 76, 164-174.
42. Xu, J. F., Wang, L. P., Ridgway, D., Gu, T. Y. & Moo-Young, M. (2000). Increased heterologous protein production in Aspergillus niger fermentation through extracellular proteases inhibition by pelleted growth. Biotechnol Prog 16, 222-227.
43. Yoder, W. T. & Lembeck, J. (2004). Heterologous expression and protein secretion in filamentous fungi. In Advances in Fungal Biotechnology for Industry, Agriculture, and Medicine, pp. 201-219. Edited by J. S. Tkacz & L. Lange. New York: Kluwer Academic/Plenum Publishers.
44. Zheng, X. F., Kobayashi, Y. & Takeuchi, M. (1998). Construction of a low-serine-type-carboxypeptidase-producing mutant of Aspergillus oryzae by the expression of antisense RNA and its use as a host for heterologous protein secretion. Appl Microbiol Biotechnol 49, 39-44.