Construction of a low-serine-type-carboxypeptidase-producing mutant of Aspergillus oryzae by the expression of antisense RNA and its use as a host for heterologous protein secretion

Applied Microbiology and Biotechnology

Volumn 49, Issue 1, 1998, Pages 39-44

  Zheng, X F ^a   Kobayashi, Y ^a   Takeuchi, M ^a  

^a TOKYO UNIVERSITY OF AGRICULTURE AND TECHNOLOGY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

CARBOXYPEPTIDASE; COMPLEMENTARY RNA; LYSOZYME;

ARTICLE; ASPERGILLUS ORYZAE; CONTROLLED STUDY; DEGRADATION; ENZYME ACTIVITY; FUNGUS MUTANT; GEL FILTRATION; GENE EXPRESSION REGULATION; IN VITRO STUDY; NONHUMAN; PROTEIN EXPRESSION; STRUCTURAL GENE;

ASPERGILLUS ORYZAE; BLOTTING, SOUTHERN; CARBOXYPEPTIDASES; HUMANS; MURAMIDASE; MUTATION; RNA, ANTISENSE; SERINE; TRANSFORMATION, GENETIC;

ASPERGILLUS ORYZAE; FUNGI;

EID: 0031963541     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s002530051134     Document Type: Article

References (27)

1. Archer DB, Mackenzie DA, Jeenes DJ, Roberts IN (1992) Proteolytic degradation of heterologous proteins expressed in Aspergillus niger. Biotechnol Lett 14: 357-362
2. Archer DB, Jeenes DJ, Mackenzie DA (1994) Strategies for improving heterologous protein production from filamentous fungi. Antoine van Leeuvenhoek 65: 245-250
3. Berka RM, Ward M, Wilson LJ, Hayenga KJ, Kodama KH, Carlomagno LP, Thompson SA (1990) Molecular cloning and deletion of the gene encoding aspergillopepsin A from Aspergillus awamori. Gene 86: 153-162
4. Cohen BL (1977) The proteases of Aspergillus. In: Smith JE, Pateman JA (eds) Genetics and physiology of Aspergillus. Academic Press, New York, pp 281-292
5. Cove DJ (1979) Genetic studies of nitrate assimilation in Aspergillus nidulans. Biol Rev 54: 291-327
6. Davies RW (1994) Heterologous gene expression and protein secretion in Aspergillus. In: Martinelli SD, Kinghorn JR (eds) Aspergillus: 50 years on. Prog Ind Microbiol 29: 527-560
7. Florkin M, Stotz EH (1973) Comprehensive Biochemistry, vol. 13, Elsevier, Amsterdam, pp 26
8. Gomi K, Iimura Y, Hara S (1987) Integrative transformation of Aspergillus oryzae with a plasmid containing the Aspergillus nidulans arg B gene. Agric Biol Chem 51: 2549-2555
9. Hintz WE, Kalsner I, Plawinski E, Guo Z, Lagosky PA (1995) Improved gene expression in Aspergillus nidulans. Can J Bot 73 [Suppl]: S876-884
10. Ichishima E (1970) Purification and mode of assay for acid proteinase of Aspergillus saitoi Methods Enzymol 19: 397-406
11. Iimura Y, Gomi K, Uzu H, Hara S (1987) Transformation of Aspergillus oryzae through plasmid mediated complementation of the methionine-auxotrophic mutation. Agric Biol Chem 51: 323-328
12. Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680-685
13. Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning. A laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, pp 250
14. Mattern IE, Van Noort JM, Van den Berg P, Archer DB, Roberts IN, Van den Hondel CAMJJ (1992) Isolation and characterization of mutant of Aspergillus niger deficient in extracellular proteases. Mol Gen Genet 234: 332-336
15. Morsky P (1983) Turbidimetric determination of lysozyme with Micrococcus lysodeikticus cells: reexamination of reaction conditions. Anal Biochem 128: 77-85
16. Robert J, George F, Sprague JR, Herskowitz IRA (1983) Regulation of yeast mating-type interconversion: feedback control of HO gene expression by the mating-type locus. Proc Natl Acad Sci USA 80: 3035-3039
17. Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning. A laboratory manual, 2nd edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY
18. Stewart P, Whitwam RE, Kersten PJ, Cullen D, Tien M (1996) Efficient expression of a Phanerochaete chrysosporium manganese peroxidase gene in Aspergillus oryzae. Appl Environ Microbiol 62: 860-864
19. Takeuchi M (1990) Substrate specificity conversion of the acid carboxypeptidases from Aspergillus oryzae by posttranslational modification. Nippon Nogeikagaku Kaishi 64: 1378-1381
20. Takeuchi M, Ichishima E (1986) A 155 K acid carboxypeptidase O from Aspergillus oryzae. Agric Biol Chem 50: 633-638
21. Takeuchi M, Ushijima T, Ichishima E (1982) A new acid carboxypeptidase, O-1, O-2 from Aspergillus oryzae. Curr Microbiol 7: 19-23
22. Thompson SA (1991) Fungal aspartic proteinases. In: Leong SA, Berka RM (eds) Molecular industrial mycology: system and applications in filamentous fungi. Dekker, New York, pp 107-128
23. Tsuchiya K, Tada S, Gomi K, Kitamoto K, Kumagai C, Jigaini Y, Tamura G (1992) High level expression of the synthetic human lysozyme gene in Aspergillus oryzae. Appl Microbiol Biotechnol 38: 109-114
24. Tsuchiya K, Nagashima T, Yamamoto Y, Gomi K, Kitamoto K, Kumagai C, Tamura G (1994) High level secretion of calf chymosin using a glucoamylase-prochymosin fusion gene in Aspergillus oryzae. Biosci Biotechnol Biochem 58: 895-899
25. Van den Hombergh JPTW, Van de Vondervoort PJI. Van der Heijden NCBA, Visser J (1995) New protease mutants in Aspergillus niger result in strongly reduced in vitro degradation of target proteins: genetical and biochemical characterization of seven complementation groups. Curr Genet 28: 299-308
26. Verdoes JC, Punt PJ, Hondel CAMJJ van den (1995) Molecular genetics strain improvement for the overproduction of fungal protein by filamentous fungi. Appl Microbiol Biotechnol 43: 195-205
27. Yelton MM, Hamer JE, Timberlake WE (1984) Transformation of Aspergillus nidulans by using a trpC plasmid. Proc Natl Acad Sci USA 81: 1370-1374