Enhanced heterologous protein production in Aspergillus niger through pH control of extracellular protease activity

Biochemical Engineering Journal

Volumn 8, Issue 3, 2001, Pages 187-193

  O'Donnell, Dara ^a   Wang, Liping ^a   Xu, Jianfeng ^a   Ridgway, Darin ^a   Gu, Tingyue ^a   Moo Young, Murray ^a,b  

^a Ohio University   (United States)

^b UNIVERSITY OF WATERLOO   (Canada)

Author keywords

Fermentation; Filamentous fungi; Glucoamylase (GLA); Green fluorescent protein (GFP); Protease inhibition

Indexed keywords

GLUCAN 1,4 ALPHA GLUCOSIDASE; GLUCOSE; GREEN FLUORESCENT PROTEIN; HYBRID PROTEIN; PROTEINASE; RECOMBINANT PROTEIN;

ARTICLE; ASPERGILLUS NIGER; BIOREACTOR; ENZYME ACTIVITY; ENZYME INHIBITION; ENZYME RELEASE; FERMENTATION; GENETIC RECOMBINATION; NONHUMAN; PH; PRIORITY JOURNAL; PROTEIN SYNTHESIS; STRAIN DIFFERENCE;

ASPERGILLUS NIGER; FUNGI;

EID: 0034764850     PISSN: 1369703X     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1369-703X(01)00102-4     Document Type: Article

References (28)

1. The molecular biology of secreted enzyme production by fungi
2. Expression of an Erwinia pectate lyase in three species of Aspergillus
3. Molecular cloning and deletion of the gene encoding Aspergillopepsin A from Aspergillus awamori
4. Genetic analysis and the construction of master strains for assignment of genes to six linkage groups in Aspergillus niger
5. Secretion of heterologous proteins by Aspergillus niger: Production of active interleukin-6 in a protease deficient mutant by KEX2-like processing of a glucoamylase-hIL6 fusion protein
6. The neutral and alkaline proteases of Aspergillus nidulans
7. Purification and characterization of two serine carboxypeptidases from Aspergillus niger and their use in C-terminal sequencing of proteins and peptide synthesis
8. Efficient production of secreted proteins by Aspergillus: Progress, limitations and prospects
9. Increased heterologous protein production in Aspergillus niger fermentation through extracellular proteases inhibition by pelleted growth
10. Kinetics of cell growth and heterologous glucoamylase production in recombinant Aspergillus nidulans
11. Inhibition of extracellular protease secretion by Aspergillus niger using cell immobilization
12. Isolation and characterization of mutants of Aspergillus niger deficient in extracellular proteases
13. Heterologous gene expression in A. niger: A glucoamylase-porcine pancreatic prophospholipase A2 fusion protein is secreted and processed to yield mature enzyme
14. New protease mutants in Aspergillus niger result in strongly reduced in vitro degradation of target proteins: Genetic and biochemical characterization of seven complementation groups
15. Aspergillus as a host for heterologous protein production: The problem of proteases
16. Development of a homologous transformation system for Aspergillus niger based on the pyrG gene
17. Heterotogous gene expression in Aspergillus: Analysis of chymosin production in single-copy transformants of A. niger
18. Molecular genetic strain improvement for the overproduction of fungal proteins by filamentous fungi
19. Aggregation of spores biomass of Phanerochaete chrysosporium in liquid culture and the effect of anionic polymers on this process
20. Improved production of chymosinin in Aspergillus by expression as a glucoamylase-chymosin fusion
21. Optimization and stability of glucoamylase production by recombinant strains of Aspergillus niger in chemostat culture
22. Effect of culture conditions and induction strategies on production of human interleukin-6 by a recombinant Aspergillus nidulans strain
23. Protein production using recombinant yeast in an immobilized-cell-film airlift reactor
24. Biotechnology review: The growth of molds in the form of pellets - A literature review
25. Morphology and growth of Aspergillus niger ATCC 26036 cultivated at several shear rates
26. Effect of agitation on growth and enzyme production of Trichoderma reesei in batch fermentation
27. Understanding the morphology of fungi