Insights into editing from an Ile-tRNA synthetase structure with tRNA(Ile) and mupirocin

Science

Volumn 285, Issue 5430, 1999, Pages 1074-1077

  Silvian, Laura F ^a,b,c   Wang, Jimin ^a   Steitz, Thomas A ^a,b  

^a YALE UNIVERSITY   (United States)

^b HOWARD HUGHES MEDICAL INSTITUTE   (United States)

^c HARVARD MEDICAL SCHOOL   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ISOLEUCINE TRANSFER RNA; ISOLEUCINE TRANSFER RNA LIGASE; PSEUDOMONIC ACID;

ARTICLE; BINDING SITE; CRYSTAL STRUCTURE; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME CONFORMATION; ENZYME STRUCTURE; HYDROLYSIS; NONHUMAN; PRIORITY JOURNAL; RNA EDITING; STAPHYLOCOCCUS AUREUS;

ACYLATION; ADENOSINE MONOPHOSPHATE; AMINO ACIDS; BINDING SITES; CRYSTALLOGRAPHY, X-RAY; DNA-DIRECTED DNA POLYMERASE; GLUTAMATE-TRNA LIGASE; ISOLEUCINE; ISOLEUCINE-TRNA LIGASE; MODELS, MOLECULAR; MUPIROCIN; NUCLEIC ACID CONFORMATION; OLIGOPEPTIDES; PROTEIN CONFORMATION; PROTEIN STRUCTURE, SECONDARY; RNA, TRANSFER, GLN; RNA, TRANSFER, ILE; STAPHYLOCOCCUS AUREUS; SUBSTRATE SPECIFICITY;

BACTERIA (MICROORGANISMS); STAPHYLOCOCCUS AUREUS;

EID: 0033551859     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.285.5430.1074     Document Type: Article

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34. Ile gene used for tRNA transcription; T. Terwilliger, R. Sweet, L. Berman, and C. Ogata for access to beamlines at Brookhaven National Laboratory, and D. Thiel for Beamlines at Cornell High Energy Synchrotron Source. We thank C. Cornell, S. Kamtekar, G. Cheetham, C. Brautigam, J. Ippolito, P. Klosterman, N. Ban, S. Szep, V. Korada, and J. Pata for help collecting data. Coordinates of the IleRS:tRNA Mupirocin complex and the structure factor amplitudes have been deposited in the Protein Data Bank (accession codes PDB 1QU2 and PDB 1QU3) and in Research Collaborating for Structural Bioinformatics at the University of New Jersey, Rutgers (RCSB009293).