Gene cloning, protein characterization, and alteration of product selectivity for the trehalulose hydrolase and trehalulose synthase from "Pseudomonas mesoacidophila" MX-45

Applied and Environmental Microbiology

Volumn 75, Issue 22, 2009, Pages 7026-7036

  Watzlawick, Hildegard ^a   Mattes, Ralf ^a  

^a UNIVERSITY OF STUTTGART   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

CLONE CELLS; CLONING; ESCHERICHIA COLI; GENES; GLUCOSE; ISOMERS;

CATALYTIC EFFICIENCIES; GLUCOSE PRODUCTION; HYDROLYTIC REACTIONS; NATURALLY OCCURRING; NORTHERN BLOT ANALYSIS; PRODUCT SELECTIVITIES; PRODUCT SPECIFICITY; PROTEIN CHARACTERIZATION;

SUGAR (SUCROSE);

HYDROLASE; TREHALULOSE HYDROLASE; TREHALULOSE SYNTHASE; UNCLASSIFIED DRUG;

CATALYSIS; CHROMOSOME; COLIFORM BACTERIUM; DNA; ENZYME ACTIVITY; GENE EXPRESSION; GENETIC ANALYSIS; HYDROLYSIS; MUTATION; PROTEIN; SUGAR;

ARTICLE; CONTROLLED STUDY; CRYSTAL STRUCTURE; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; MOLECULAR CLONING; NONHUMAN; NORTHERN BLOTTING; PROTEIN ANALYSIS; PSEUDOMONAS; PSEUDOMONAS MESOACIDOPHILA;

ESCHERICHIA COLI; PSEUDOMONAS MESOACIDOPHILA;

EID: 70449434666     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.01781-09     Document Type: Article

References (50)

1. Ahn, S. J., J. H. Yoo, H. C. Lee, S. Y. Kim, B. S. Noh, J. H. Kim, and J. K. Lee. 2003. Enhanced conversion of sucrose to isomaltulose by a mutant of Erwinia rhapontici. Biotechnol. Lett. 25:1179-1183.
2. Altenbuchner, J., P. Viell, and I. Pelletier. 1992. Positive selection vectors based on palindromic DNA sequences. Methods Enzymol. 216:457-466. (Pubitemid 23041090)
3. Aroonnual, A., T. Ninhira, T. Seki, and W. Panbangred. 2007. Role of several key residues in the catalytic activity of sucrose isomerase from Klebsiella pneumoniae NK33-98-8. Enzyme Microb. Technol. 40:1221-1227.
4. Bendtsen, J. D., H. Nielsen, G. von Heijne, and S. Brunak. 2004. Improved prediction of signal peptides: SignalP 3.0. J. Mol. Biol. 340:783-795.
5. Börnke, F., M. Hajirezaei, and U. Sonnewald. 2001. Cloning and characterization of the gene cluster for palatinose metabolism from the phytopathogenic bacterium Erwinia rhapontici. J. Bacteriol. 183:2425-2430. (Pubitemid 32249749)
6. Bradford, M. M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72:248-254.
7. Cheetham, P. S., C. E. Imber, and J. Isherwood. 1982. The formation of isomaltulose by immobilized Erwinia rhapontici. Nature 299:628-631.
8. Cho, M. H., S. E. Park, J. K. Lim, J. S. Kim, J. H. Kim, D. Y. Kwon, and C. S. Park. 2007. Conversion of sucrose into isomaltulose by Enterobacter sp. FMB1, an isomaltulose-producing microorganism isolated from traditional Korean food. Biotechnol. Lett. 29:453-458.
9. Chung, C. T., S. L. Niemela, and R. H. Miller. 1989. One-step preparation of competent Escherichia coli: transformation and storage of bacterial cells in the same solution. Proc. Natl. Acad. Sci. USA 86:2172-2175.
10. Gouffi, K., N. Pica, V. Pichereau, and C. Blanco. 1999. Disaccharides as a new class of nonaccumulated osmoprotectants for Sinorhizobium meliloti. Appl. Environ. Microbiol. 65:1491-1500.
11. Hamada, S. 2002. Role of sweeteners in the etiology and prevention of dental caries. Pure Appl. Chem. 74:1293-1300.
12. Hendrix, D. L., and Y. Wei. 1992. Detection and elimination of honeydew excreted by the sweet potato white fly feeding upon cotton, p. 671-673. In J. M. Brown and T. C. Nelson (ed.), Proceedings of the Beltwide Cotton Production Conference, vol.2. National Cotton Council, Memphis, TN.
13. Hequet, E., and N. Abidi. 2002. Processing sticky cotton: implication of trehalulose in residue build-up. J. Cotton Sci. 6:77-90. (Pubitemid 38907983)
14. Hequet, E., N. Abidi, and D. Ethridge. 2005. Processing sticky cotton. Textile Res. J. 75:402.
15. Kawai, K., Y. Okuda, and K. Yamashita. 1985. Changes in blood glucose and insulin after an oral palatinose administration in normal subjects. Endocrinol. Jpn. 32:933-936.
16. Krastanov, A., and T. Yoshida. 2003. Production of palatinose using Serratia plymuthica cells immobilized in chitosan. J. Ind. Microbiol. Biotechnol. 30:593-598.
17. Laemmli, U. K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680-685.
18. Lantero, O. J., Jr., and J. K. Shetty. June 1998. Enzyme composition for the treatment of sticky cotton fiber and method for the treatment of sticky cotton fiber with such enzyme composition. U.S. patent 5770437.
19. Lee, H. C., J. H. Kim, S. Y. Kim, and J. K. Lee. 2008. Isomaltose production by modification of the fructose-binding site on the basis of the predicted structure of sucrose isomerase from "Protaminobacter rubrum." Appl. Environ. Microbiol. 74:5183-5194.
20. Li, X., D. Zhang, F. Chen, J. Ma, Y. Dong, and L. Zhang. 2004. Klebsiella singaporensis sp. nov., a novel isomaltulose-producing bacterium. Int. J. Syst. Evol. Microbiol. 54:2131-2136.
21. Lichtenthaler, F. W., and S. Peters. 2004. Carbohydrates as green raw materials for the chemical industry. C. R. Chimie 7:65-90.
22. Lina, B. A., D. Jonker, and G. Kozianowski. 2002. Isomaltulose (palatinose): a review of biological and toxicological studies. Food Chem. Toxicol. 40:1375-1381.
23. Matsukubo, T., and I. Takazoe. 2006. Sucrose substitutes and their role in caries prevention. Int. Dent. J. 56:119-130.
24. Mattes, R., K. Klein, H. Schiweck, M. Kunz, and M. Munir. July 1995. Preparation of acariogenic sugar substitutes. International patent WO 95/20047 A3.
25. McAllister, M., C. T. Kelly, E. Doyle, and W. M. Fogarty. 1990. The isomaltulose synthesizing enzyme of Serratia plymuthica. Biotechnol. Lett. 12:667-672.
26. Miyata, Y., T. Sugitani, K. Tsuyuki, T. Ebashi, and Y. Nakajima. 1992. Isolation and characterisation of Pseudomonas mesoacidophila producing trehalulose. Biosci. Biotechnol. Biochem. 56:1680-1681.
27. Nagai, Y., T. Sugitani, and K. Tsuyuki. 1994. Characterization of alpha-glucosyltransferase from Pseudomonas mesoacidophila MX-45. Biosci. Biotechnol. Biochem. 58:1789-1793.
28. Nagai-Miyata, Y., K. Tsuyuki, T. Sugitani, T. Ebashi, and Y. Nakajima. 1993. Isolation and characterization of a trehalulose-producing strain of Agrobacterium. Biosci. Biotechnol. Biochem. 57:2049-2053.
29. Ravaud, S., X. Robert, H. Watzlawick, R. Haser, R. Mattes, and N. Aghajari. 2007. Trehalulose synthase native and carbohydrate complexed structures provide insights into sucrose isomerization. J. Biol. Chem. 282:28126-28136.
30. Ravaud, S., X. Robert, H. Watzlawick, R. Haser, R. Mattes, and N. Aghajari. 2009. Structural determinants of product specificity of sucrose isomerases. FEBS Lett. 583:1964-1968.
31. Ravaud, S., H. Watzlawick, R. Haser, R. Mattes, and N. Aghajari. 2005. Expression, purification, crystallization and preliminary X-ray crystallographic studies of the trehalulose synthase MutB from Pseudomonas mesoacidophila MX-45. Acta Crystallogr. F 61:100-103.
32. Ravaud, S., H. Watzlawick, R. Haser, R. Mattes, and N. Aghajari. 2006. Overexpression, purification, crystallization and preliminary diffraction studies of the Protaminobacter rubrum sucrose isomerase SmuA. Acta Crystallogr. F 62:74-76.
33. Salvucci, M. E. 2003. Distinct sucrose isomerases catalyze trehalulose synthesis in whiteflies, Bemisia argentifolii, and Erwinia rhapontici. Comp. Biochem. Physiol. B 135:385-395.
34. Sambrook, J., and D. W. Russell. 2001. Molecular cloning: a laboratory manual, 3rd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
35. Schiweck, H., M. Munir, K. M. Rapp, B. Schneider, and M. Vogel. 1990. New developments in the use of sucrose as an industrial bulk chemical. Zuckerindustrie 115:555-565.
36. Sedlmeier, R., and J. Altenbuchner. 1992. Cloning and DNA sequence analysis of the mercury resistance genes of Streptomyces lividans. Mol. Gen. Genet. 236:76-85.
37. Stumpp, T., B. Wilms, and J. Altenbuchner. 2000. Ein neues L-Rhamnose-induzierbares Expressionssystem. Biospektrum 1:33-36.
38. Takazoe, I. 1989. Palatinose-an isomeric alternative to sucrose, p. 143-167. In T. H. Grenby (ed.), Progress in sweeteners. Elsevier, Barking, United Kingdom.
39. Thompson, J., S. A. Robrish, S. Immel, F. W. Lichtenthaler, B. G. Hall, and A. Pikis. 2001. Metabolism of sucrose and its five linkage-isomeric alpha-D-glucosyl-D-fructoses by Klebsiella pneumoniae. Participation and properties of sucrose-6-phosphate hydrolase and phospho-alpha-glucosidase. J. Biol. Chem. 276:37415-37425.
40. Thompson, J., S. A. Robrish, A. Pikis, A. Brust, and F. W. Lichtenthaler. 2001. Phosphorylation and metabolism of sucrose and its five linkage-isomeric alpha-D-glucosyl-D-fructoses by Klebsiella pneumoniae. Carbohydr. Res. 331:149-161. (Pubitemid 32226395)
41. Tsuyuki, K., T. Sugitani, Y. Miyata, T. Ebashi, and Y. Nakajima. 1992. Isolation and characterization of isomaltulose-producing and trehaulose-producing bacteria from Thailand soil. J. Gen. Appl. Microbiol. 38:483-490.
42. Veronese, T., and P. Perlot. 1998. Proposition for the biochemical mechanism occurring in the sucrose isomerase active site. FEBS Lett. 441:348-352. (Pubitemid 29065312)
43. Volff, J. N., C. Eichenseer, P. Viell, W. Piendl, and J. Altenbuchner. 1996. Nucleotide sequence and role in DNA amplification of the direct repeats composing the amplifiable element AUD1 of Streptomyces lividans 66. Mol. Microbiol. 21:1037-1047.
44. Watanabe, K., K. Kitamura, H. Iha, and Y. Suzuki. 1990. Primary structure of the oligo-1,6-glucosidase of Bacillus cereus ATCC7064 deduced from the nucleotide sequence of the cloned gene. Eur. J. Biochem. 192:609-620.
45. Weidenhagen, R., and S. Lorenz. 1957. Palatinose 6-(α- glucopyranosido)-fructofuranose, ein neues bakterielles Umwandlungsprodukt der Saccharose. Z. Zuckerindust. 7:533-534.
46. Wu, L., and R. G. Birch. 2004. Characterization of Pantoea dispersa UQ68J: producer of a highly efficient sucrose isomerase for isomaltulose biosynthesis. J. Appl. Microbiol. 97:93-103.
47. Wu, L., and R. G. Birch. 2005. Characterization of the highly efficient sucrose isomerase from Pantoea dispersa UQ68J and cloning of the sucrose isomerase gene. Appl. Environ. Microbiol. 71:1581-1590.
48. Zhang, D., N. Li, S. M. Lok, L. H. Zhang, and K. Swaminathan. 2003. Isomaltulose synthase (PalI) of Klebsiella sp. LX3. Crystal structure and implication of mechanism. J. Biol. Chem. 278:35428-35434.
49. Zhang, D., N. Li, K. Swaminathan, and L. H. Zhang. 2003. A motif rich in charged residues determines product specificity in isomaltulose synthase. FEBS Lett. 534:151-155. (Pubitemid 36084533)
50. Zhang, D., X. Li, and L. H. Zhang. 2002. Isomaltulose synthase from Klebsiella sp. strain LX3: gene cloning and characterization and engineering of thermostability. Appl. Environ. Microbiol. 68:2676-2682.