Structure of a switchable subtilisin complexed with a substrate and with the activator azide

Biochemistry

Volumn 48, Issue 43, 2009, Pages 10389-10394

  Gallagher, Travis ^a,b   Ruan, Biao ^a,c   London, Mariya ^a,c   Bryan, Molly A ^a,c   Bryan, Philip N ^a  

^a UNIVERSITY OF MARYLAND BIOTECHNOLOGY INSTITUTE   (United States)

^b NATIONAL INSTITUTE OF STANDARDS AND TECHNOLOGY   (United States)

^c Potomac Affinity Proteins LLC   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ACTIVE SITE; ACTIVITY MEASUREMENTS; ACTIVITY-DEPENDENT; BACILLUS AMYLOLIQUEFACIENS; C-TERMINUS; CATALYTIC MECHANISMS; CATALYTIC NUCLEOPHILES; MODIFIED ENZYMES; RESOLUTION STRUCTURE; SERINE PROTEASE; SUBSTRATE BINDING; SUBSTRATE COMPLEXES; SUBTILISIN; SWITCHABLE;

AMINO ACIDS; BACTERIOLOGY; ENZYME ACTIVITY; ENZYMES; NEGATIVE IONS; SUBSTRATES;

CRYSTAL STRUCTURE;

ALANINE DERIVATIVE; ANION; AZIDE; NUCLEOPHILE; PROTEINASE; SUBTILISIN;

ARTICLE; BACILLUS AMYLOLIQUEFACIENS; BINDING AFFINITY; BIOENGINEERING; BIOTECHNOLOGY; CARBOXY TERMINAL SEQUENCE; CATALYSIS; CRYSTAL STRUCTURE; ENZYME ACTIVITY; ENZYME BINDING; ENZYME STRUCTURE; ENZYME SUBSTRATE; GENE MUTATION; NONHUMAN; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN INTERACTION; PROTEIN STABILITY;

ANIONS; AZIDES; BACILLUS; BACTERIAL PROTEINS; CRYSTALLOGRAPHY, X-RAY; FLUORIDES; KINETICS; MODELS, BIOLOGICAL; MUTATION; PROTEIN BINDING; PROTEIN STRUCTURE, SECONDARY; SUBTILISINS;

BACILLUS AMYLOLIQUEFACIENS;

EID: 70350508349     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi900577n     Document Type: Article

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