Engineering the independent folding of the subtilisin BPN' prodomain: Analysis of two-state folding versus protein stability

Biochemistry

Volumn 36, Issue 34, 1997, Pages 10414-10421

  Ruvinov, Sergei ^a   Wang, Lan ^a   Ruan, Biao ^a   Almog, Orna ^b   Gilliland, Gary L ^b   Eisenstein, Edward ^b   Bryan, Philip N ^a  

^a UNIVERSITY OF MARYLAND BIOTECHNOLOGY INSTITUTE   (United States)

^b NATIONAL INSTITUTE OF STANDARDS AND TECHNOLOGY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACID; SUBTILISIN;

AMINO ACID SEQUENCE; ARTICLE; CIRCULAR DICHROISM; CRYSTAL STRUCTURE; DIFFERENTIAL SCANNING CALORIMETRY; EQUILIBRIUM CONSTANT; GENE; MUTATION; NONHUMAN; PRIORITY JOURNAL; PROTEIN DENATURATION; PROTEIN FOLDING; PROTEIN SECONDARY STRUCTURE; PROTEIN STABILITY; SPECTROSCOPY; THERMODYNAMICS;

BACILLUS SUBTILIS; CALORIMETRY, DIFFERENTIAL SCANNING; CIRCULAR DICHROISM; CLONING, MOLECULAR; ENZYME STABILITY; ESCHERICHIA COLI; HYDROGEN-ION CONCENTRATION; KINETICS; MUTATION; OSMOLAR CONCENTRATION; PROTEIN BINDING; PROTEIN DENATURATION; PROTEIN ENGINEERING; PROTEIN FOLDING; RECOMBINANT PROTEINS; SUBTILISINS; TEMPERATURE; THERMODYNAMICS; ULTRACENTRIFUGATION;

EID: 0030803268     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi9703958     Document Type: Article

References (34)

1. Alexander, P., Fahnestock, S., Lee, T., Orban, J., & Bryan, P. (1992) Biochemistry 31, 3597-3603.
2. Bai, Y., & Englander, S. W. (1996) Proteins: Struct., Funct., Genet. 24, 145-151.
3. Baldwin, R. L. (1986) Proc. Natl. Acad. Sci. U.S.A. 83, 8069-8072.
4. Becktel, W. J., & Schellman, J. A. (1987) Biopolymers 26, 1859-1877.
5. Betz, S. F., Raleigh, D. P., & DeGrado, W. F. (1993) Curr. Opin. Struct. Biol. 3,
6. Brandts, J. F. (1964) J. Am. Chem. Soc. 86, 4291-4301.
7. Brooks, I. S., Soneson, K. K., & Hensley, P. (1993) Biophys. J. 64, A244.
8. Bryan, P., Wang, L., Hoskins, J., Ruvinov, S., Strausberg, S., Alexander, P., Almog, O., Gilliland, G., & Gallagher, T. D. (1995) Biochemistry 34, 10310-10318.
9. Buckler, D. R., Haas, E., & Scheraga, H. A. (1995) Biochemistry 34, 15965-15978.
10. Dill, K. A., & Shortle, D. (1991) Annu. Rev. Biochem. 60, 795-825.
11. Ellis, K. J., & Morrison, J. F. (1982) Methods Enzymol. 87, 405-426.
12. Gallagher, T. D., Gilliland, G., Wang, L., & Bryan, P. (1995) Structure 3, 907-914.
13. Goodman, E. M., & Kim, P. S. (1989) Biochemistry 28, 4343-4347.
14. Hu, C-Q., Kitamura, S., Tanaka, A., & Sturtevant, J. M. (1992) Biochemistry 31, 1643-1647.
15. Ikemura, H., Takagi, H., & Inouye, M. (1987) J. Biol. Chem. 262, 7859-7864.
16. Kamtekar, S., Schiffer, J. M., Xiong, H., Babik, J. M., & Hecht, M. H. (1993) Science 262, 1680-1685.
17. Lattman, E. E., & Rose, G. D. (1993) Proc. Natl. Acad. Sci. U.S.A. 90, 439-441.
18. Livingstone, J. R., Spolar, R. S., & Record, T. M. (1991) Biochemistry 30, 4237-4244.
19. Makhatadze, G. I., & Privalov, P. L. (1995) Adv. Protein Chem. 47, 307-425.
20. Matthews, B. W. (1987) Biochemistry 26, 6885-6888.
21. Merutka, G., Shalongo, W., & Stellwagen, E. (1991) Biochemistry 30, 4225-4248.
22. Orban, J., Alexander, P., Bryan, P., & Khare, D. (1995) Biochemistry 34, 15291-15300.
23. Pace, C. N., & Tanford, C. (1968) Biochemistry 7, 198-208.
24. Privalov, P. L. (1979) Adv. Protein Chem. 33, 167-241.
25. Privalov, P. L., & Gill, S. J. (1988) Adv. Protein Chem. 39, 191-234.
26. Privalov, P. L., & Khechinashvili, N. N. (1974) J. Mol. Biol. 86, 665-684.
27. Schwarz, F. P., & Kirchhoff, W. H. (1988) Thermochim. Acta 128, 267-295.
28. Shirley, B. A., Stanssens, P., Ulrich, H., & Pace, C. N. (1992) Biochemistry 31, 725-732.
29. Shortle, D. (1996) Protein Sci. 5, 991-1000.
30. Shortle, D., Meeker, A. K., & Freire, E. (1988) Biochemistry 27, 4761-4768.
31. Strausberg, S., Alexander, P., Wang, L., Schwarz, F., & Bryan, P. (1993) Biochemistry 32, 8112-8119.
32. Strausberg, S., Alexander, P., Gallagher, D. T., Gilliland, G., Barnett, B. L., & Bryan, P. (1995) Bio/Technology 13, 669-673.
33. Wang, L., Ruvinov, S., Strausberg, S., Gallagher, T. D., Gilliland, G., & Bryan, P. (1995) Biochemistry 15, 415-15, 420.
34. Wiseman, T., Williston, S., Brandts, J. F., & Lin, L.-N. (1989) Anal. Biochem. 179, 131-137.