Single-quantum dot imaging with a photon counting camera

Current Pharmaceutical Biotechnology

Volumn 10, Issue 5, 2009, Pages 543-558

  Michalet, X ^a   Colyer, R A ^a   Antelman, J ^a   Siegmund, O H W ^b   Tremsin, A ^b   Vallerga, J V ^b   Weiss, S ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

FCS; Fluorescence lifetime; Particle tracking; Phasor analysis; Photon counting; Quantum dot; Single molecule; TCSPC; Wide field detector

Indexed keywords

QUANTUM DOT;

ACCURACY; ANALYTIC METHOD; ARTICLE; CAMERA; CONFOCAL MICROSCOPY; FLUORESCENCE MICROSCOPY; FLUORESCENCE RESONANCE ENERGY TRANSFER; IMAGE INTENSIFIER; IMAGING; MATHEMATICAL ANALYSIS; PHOTOACTIVATION; PHOTON; PHOTON DETECTION; PHOTON EMISSION TOMOGRAPHY; PROCESS CONTROL; PROCESS DESIGN; SENSITIVITY ANALYSIS; SIGNAL NOISE RATIO; SINGLE MOLECULE FLUORESCENCE MICROSCOPY; COMPUTER ASSISTED DIAGNOSIS; ELECTRON; EQUIPMENT DESIGN; NANOTECHNOLOGY; SPECTROFLUOROMETRY;

ELECTRONS; EQUIPMENT DESIGN; MICROSCOPY, FLUORESCENCE; NANOTECHNOLOGY; PHOTONS; QUANTUM DOTS; RADIOGRAPHIC IMAGE INTERPRETATION, COMPUTER-ASSISTED; SPECTROMETRY, FLUORESCENCE;

EID: 70350448635     PISSN: 13892010     EISSN: 18734316     Source Type: Journal    
DOI: 10.2174/138920109788922100     Document Type: Article

References (37)

1. Weiss, S. (1999) Fluorescence spectroscopy of single bio-molecules. Science, 283 1676-1683
2. Weiss, S. (2000) Measuring conformational dynamics of bio-molecules by single molecule fluorescence spectroscopy. Nat. Struct. Biol., 7 (9), 724-729 (Pubitemid 30688652)
3. Michalet, X. and Weiss, S. (2002) Single-molecule spectroscopy and microscopy. C.R. Phys., 3 (5), 619-644
4. Michalet, X.; Weiss, S. and Jäger, M. (2006) Single-molecule fluorescence studies of protein folding and conformational dynamics. Chem. Rev., 106 (5), 1785-1813 (Pubitemid 43792781)
5. Betzig, E.; Patterson, G. H.; Sougrat, R.; Lindwasser, O. W.; Olenych, S.; Bonifacino, J. S.; Davidson, M. W.; Lippincott- Schwartz, J. and Hess, H. F. (2006) Imaging intracellular fluorescent proteins at nanometer resolution. Science, 313 (5793), 1642-1645 (Pubitemid 44414039)
6. Bates, M.; Huang, B.; Dempsey, G. T. and Zhuang, X. (2007) Multicolor super-resolution imaging with photo-switchable fluorescent probes. Science, 317 (5845), 1749-1753 (Pubitemid 47461839)
7. Hell, S. W. (2007) Far-field optical nanoscopy. Science, 316 (5828), 1153-1158
8. Michalet, X.; Pinaud, F. F.; Bentolila, L. A.; Tsay, J. M.; Doose, S.; Li, J. J.; Sundaresan, G.; Wu, A. M.; Gambhir, S. S. and Weiss, S. (2005) Quantum dots for live cells, in vivo imaging, and diagnostics. Science, 307 538-544 (Pubitemid 40165671)
9. Michalet, X.; Cheng, A.; Antelman, J.; Suyama, M.; Arisaka, K. and Weiss, S. (2008) Hybrid photodetector for single-molecule spectroscopy and microscopy. Proceedings of SPIE, 6862, 68620F. (Pubitemid 351653896)
10. Guerrieri, F.; Tisa, S. and Zappa, F. (2009) Fast single-photon imager acquires 1024 pixels at 100 kframe/s. Proceedings of SPIE, 7249, 72490U.
11. Siegmund, O. H. W.; Michalet, X.; Vallerga, J. V.; Jelinsky, P. and Weiss, S. (2005) Cross delay line detectors for high time resolution astronomical polarimetry and biological fluorescence imaging. IEEE Nuclear Symposium Conference Record, N14-55, 448-452
12. Michalet, X.; Siegmund, O. H. W.; Vallerga, J. V.; Jelinsky, P.; Millaud, J. E. and Weiss, S. (2006) Photon-counting H33D detector for biological fluorescence imaging. Nucl. Instr. Meth. Phys. Res. A, 567, 133-136
13. Michalet, X.; Siegmund, O. H. W.; Vallerga, J. V.; Jelinsky, P.; Millaud, J. E. and Weiss, S. (2006) A space- and time-resolved single- photon counting detector for fluorescence microscopy and spectroscopy. SPIE Proc., 6092, 60920M.
14. Michalet, X.; Siegmund, O. H. W.; Vallerga, J. V.; Jelinsky, P.; Pinaud, F. F.; Millaud, J. E. and Weiss, S. (2006) Fluorescence lifetime microscopy with a time- and space-resolved single-photon counting detector. Proceedings of SPIE, 6372, 63720E.
15. Michalet, X.; Siegmund, O. H. W.; Vallerga, J. V.; Jelinsky, P.; Millaud, J. E. and Weiss, S. (2007) Detectors for single-molecule fluorescence imaging and spectroscopy. J. Mod. Opt., 54, 239-282
16. Colyer, R.; Siegmund, O.; Tremsin, A.; Vallerga, J.; Weiss, S. and Michalet, X. (2009) Phasor-based single-molecule fluorescence lifetime imaging using a widefield photon-counting detector. Proceedings of SPIE, 7185, 71850T.
17. Nirmal, M.; Dabbousi, B. O.; Bawendi, M. G.; Macklin, J. J.; Trautman, J. K.; Harris, T. D. and Brus, L. E. (1996) Fluorescence intermittency in single cadmium selenide nanocrystals. Nature, 383, 802-804
18. Efros, A. L. and Rosen, M. (1997) Random telegraph signal in the photoluminescence intensity of a single quantum dot. Phys. Rev. Lett., 78(6), 1110-1113
19. Kuno, M.; Fromm, D. P.; Hamann, H. F.; Gallagher, A. and Nesbitt, D. J. (2000) Nonexponential 'blinking' kinetics of single CdSe quantum dots: A universal power law behavior. J. Chem. Phys., 112, 3117-3120
20. Chen, Y.; Vela, J.; Htoon, H.; Casson, J. L.; Werder, D. J.; Bussian, D. A.; Klimov, V. I. and Hollingsworth, J. A. (2008) "Giant" multishell CdSe nanocrystal quantum dots with suppressed blinking. J. Am. Chem. Soc., 130(15), 5026-5027
21. Mahler, B.; Spinicelli, P.; Buil, S.; Quelin, X.; Hermier, J. P. and Dubertret, B. (2008) Towards non-blinking colloidal quantum dots. Nat. Mater., 7(8), 659-664
22. Dahan, M.; Laurence, T.; Pinaud, F.; Chemla, D. S.; Alivisatos, A. P.; Sauer, M. and Weiss, S. (2001) Time-gated biological imaging using colloidal quantum dots. Opt. Lett., 26 (11), 825-828 (Pubitemid 33693588)
23. Schlegel, G.; Bohnenberger, J.; Potapova, I. and Mews, A. (2002) Fluorescence decay time of single semiconductor nanocrystals. Phys. Rev. Lett., 88 (13), 137401. (Pubitemid 34435691)
24. Ulbrich, M. H. and Isacoff, E. Y. (2007) Subunit counting in membrane-bound proteins. Nat. Meth., 4 (4), 319-321
25. Verberk, R.; van Oijen, A. M. and Orrit, M. (2002) Simple model for the power-law blinking of single semiconductor nanocrystals. Phys. Rev. B, 66 233202.
26. Becker, W. Advanced time-correlated single photon couting techniques. Springer: Berlin, 2005.
27. Messin, G.; Hermier, J. P.; Giacobino, E.; Desbiolles, P. and Dahan, M. (2001) Bunching and antibunching in the fluorescence of semiconductor nanocrystals. Opt. Lett., 26(23), 1891-1893 (Pubitemid 33693135)
28. An additional factor makes using very fine binning somewhat useless. Indeed, the absolute localization accuracy of the H33D was estimated to be of the order of ∼50-100 μm (ref JMO). Since the sensitive area of diameter 27 mm is represented by ∼ 400 pixels when binning with a factor 8, each pixel represents a square of the detector with side ∼ 68 μm. Since this is close to the expected localization accuracy, there is not much localization improvement to expect by using binning factors of 2 or 1.
29. Antelman, J.; Wilking-Chang, C.; Weiss, S. and Michalet, X. (2009) Nanometer distance measurements between multicolor quantum dots. Nano Lett., 9, 2199-2205
30. Michalet, X.; Lacoste, T. D. and Weiss, S. (2001) Ultrahigh-resolution colocalization of spectrally resolvable point-like fluorescent probes. Methods, 25(1), 87-102.
31. Pinaud, F.; Michalet, X.; Iyer, G.; Margeat, E.; Moore, H.-P. and Weiss, S. (2009) Dynamic partitioning of a GPI-anchored protein in glycosphingolipid- rich microdomains imaged by single-quantum dot tracking. Traffic, 10, 671-712.
32. Clayton, A. H. A.; Hanley, Q. S. and Verveer, P. J. (2004) Graphical representation and multicomponent analysis of single-frequency fluorescence lifetime imaging microscopy data. J. Microsc., 213(1), 1-5.
33. Redford, G. I. and Clegg, R. M. (2005) Polar plot representation for frequency-domain analysis of fluorescence lifetimes. J. Fluorescence, 15(5), 805-815
34. Digman, M. A.; Caiolfa, V. R.; Zamai, M. and Gratton, E. (2008) The phasor approach to fluorescence lifetime imaging analysis. Biophys. J., 94 (2), L14-L16.
35. Iyer, G.; Michalet, X.; Chang, Y.-P.; Pinaud, F. F.; Matyas, S. E.; Payne, G. and Weiss, S. (2008) High affinity scfv-hapten pair as a tool for quantum dot labeling and tracking of single proteins in live cells. Nano Lett., 8 4618-4623
36. Laurence, T. A.; Fore, S. and Huser, T. (2006) Fast, flexible algorithm for calculating photon correlations. Opt. Lett., 31 (6), 829-831
37. Thompson, R. E.; Larson, D. R. and Webb, W. W. (2002) Precise nanometer localization analysis for individual fluorescent probes. Biophys. J., 82 2775-2783