In vivo knockdown of p85 with an antisense oligonucleotide improves insulin sensitivity in Lep ob/ob and diet-induced obese mice

Hormone and Metabolic Research

Volumn 41, Issue 10, 2009, Pages 757-761

  Moriarty, M W ^a   McCurdy, C E ^a   Janssen, R C ^a   Shaw, T ^a   Leitner, J W ^b   Friedman, J E ^a,b   Draznin, B ^b  

^a University of Colorado Denver and Children s Hospital Colorado   (United States)

^b UNIVERSITY OF COLORADO SCHOOL OF MEDICINE   (United States)

Author keywords

Insulin resistance; Obesity; PI3 kinase

Indexed keywords

ANTIDIABETIC AGENT; ANTISENSE OLIGONUCLEOTIDE; CYSTEINYLCYSTEINYLALANYLGLYCYLTHREONYLGLYCYLGLYCYLTHREONYL THREONYLTHREONYLCYSTEINYLALANYLTHREONYLTHREONYLGLYCYLTHREONYLALANYL GLYCYLCYSTEINYLCYSTEINE; GLUCOSE; INSULIN; LEPTIN; MESSENGER RNA; P85ALPHA PROTEIN; PHOSPHATIDYLINOSITOL 3 KINASE; PROTEIN SUBUNIT; SODIUM CHLORIDE; UNCLASSIFIED DRUG; RNA;

ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ANTISENSE THERAPY; ARTICLE; CONTROLLED STUDY; DIET RESTRICTION; ENZYME ACTIVATION; ENZYME ACTIVITY; GENE EXPRESSION PROFILING; GENE EXPRESSION REGULATION; GENE REPRESSION; GENE TARGETING; GLUCOSE BLOOD LEVEL; GLUCOSE TOLERANCE TEST; GLYCEMIC CONTROL; INSULIN BLOOD LEVEL; INSULIN RESISTANCE; INSULIN SENSITIVITY; INSULIN TOLERANCE TEST; LEAN BODY WEIGHT; LIPID DIET; MOUSE; NONHUMAN; OBESITY; PRIORITY JOURNAL; PROTEIN DETERMINATION; PROTEIN EXPRESSION; REAL TIME POLYMERASE CHAIN REACTION; TREATMENT DURATION; WESTERN BLOTTING; ANIMAL; BLOOD; C57BL MOUSE; GENETICS; LIVER; METABOLISM; MOUSE MUTANT; PHYSIOLOGY; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SKELETAL MUSCLE;

MUS;

ANIMALS; BLOOD GLUCOSE; BLOTTING, WESTERN; GLUCOSE TOLERANCE TEST; INSULIN; INSULIN RESISTANCE; LIVER; MICE; MICE, INBRED C57BL; MICE, OBESE; MUSCLE, SKELETAL; OBESITY; OLIGONUCLEOTIDES, ANTISENSE; PHOSPHATIDYLINOSITOL 3-KINASES; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA;

EID: 70350367146     PISSN: 00185043     EISSN: 14394286     Source Type: Journal    
DOI: 10.1055/s-0029-1225363     Document Type: Article

References (22)

1. Shepherd PR, Withers DJ, Siddle K. Phosphoinositide 3-kinase: the key switch mechanism in insulin signalling. Biochem J 1998 333 471-490 [Erratum: Biochem J 1998; 3 35 (Pt 3): 711]
2. Virkamaki A, Ueki K, Kahn CR. Protein-protein interaction in insulin signaling and the molecular mechanisms of insulin resistance. J Clin Invest 1999 103 931-943
3. Ueki K, Fruman DA, Brachmann SM, Tseng YH, Cantley LC, Kahn CR. Molecular balance between the regulatory and catalytic subunits of phosphoinositide 3-kinase regulates cell signaling and survival. Mol Cell Biol 2002 22 965-977
4. Giorgino F, Pedrini MT, Matera L, Smith RJ. Specific increase in p85alpha expression in response to dexamethasone is associated with inhibition of insulin-like growth factor-I stimulated phosphatidylinositol 3-kinase activity in cultured muscle cells. J Biol Chem 1997 272 7455-7463
5. Mauvais-Jarvis F, Ueki K, Fruman DA, Hirshman MF, Sakamoto K, Goodyear LJ, Iannacone M, Accili D, Cantley LC, Kahn CR. Reduced expression of the murine p85alpha subunit of phosphoinositide 3-kinase improves insulin signaling and ameliorates diabetes. J Clin Invest 2002 109 141-149
6. Terauchi Y, Tsuji Y, Satoh S, Minoura H, Murakami K, Okuno A, Inukai K, Asano T, Kaburagi Y, Ueki K, Nakajima H, Hanafusa T, Matsuzawa Y, Sekihara H, Yin Y, Barrett JC, Oda H, Ishikawa T, Akanuma Y, Komuro I, Suzuki M, Yamamura K, Kodama T, Suzuki H, Yamamura K, Kodama T, Suzuki H, Koyasu S, Aizawa S, Tobe K, Fukui Y, Yazaki Y, Kadowaki T. Increased insulin sensitivity and hypoglycaemia in mice lacking the p85 alpha subunit of phosphoinositide 3-kinase. Nature Genet 1999 21 230-235
7. Ueki K, Algenstaedt P, Mauvais-Jarvis F, Kahn CR. Positive and negative regulation of phosphoinositide 3-kinase-dependent signaling pathways by three different gene products of the p85alpha regulatory subunit. Mol Cell Biol 2000 20 8035-8046
8. Ueki K, Fruman DA, Yballe CM, Fasshauer M, Klein J, Asano T, Cantley LC, Kahn CR. Positive and negative roles of p85 alpha and p85 beta regulatory subunits of phosphoinositide 3-kinase in insulin signaling. J Biol Chem 2003 278 48453-48466
9. Bandyopadhyay GK, Yu JG, Ofrecio J, Olefsky JM. Increased p85/55/50 Expression and Decreased Phosphotidylinositol 3-Kinase Activity in Insulin-Resistant Human Skeletal Muscle. Diabetes 2005 54 2351-2359
10. Friedman JE, Ishizuka T, Shao J, Huston L, Highman T, Catalano P. Impaired glucose transport and insulin receptor tyrosine phosphorylation in skeletal muscle from obese women with gestational diabetes. Diabetes 1999 48 1807-1814
11. Cornier MA, Bessesen DH, Gurevich I, Leitner JW, Draznin B. Nutritional upregulation of p85alpha expression is an early molecular manifestation of insulin resistance. Diabetologia 2006 49 748-754
12. Del Rincon JP, Iida K, Gaylinn BD, McCurdy CE, Leitner JW, Barbour LA, Kopchick JJ, Friedman JE, Draznin B, Thorner MO. Growth hormone regulation of p85alpha expression and phosphoinositide 3-kinase activity in adipose tissue: mechanism for growth hormone-mediated insulin resistance. Diabetes 2007 56 1638-1646
13. Ueki K, Yballe CM, Brachmann SM, Vicent D, Watt JM, Kahn CR, Cantley LC. Increased insulin sensitivity in mice lacking p85beta subunit of phosphoinositide 3-kinase. Proc Natl Acad Sci USA 2002 99 419-424
14. Choi CS, Savage DB, Kulkarni A, Yu XX, Liu ZX, Morino K, Kim S, Distefano A, Samuel VT, Neschen S, Zhang D, Wang A, Zhang XM, Kahn M, Cline GW, Pandey SK, Geisler JG, Bhanot S, Monia BP, Shulman GI. Suppression of diacylglycerol acyltransferase-2 (DGAT2), but not DGAT1, with antisense oligonucleotides reverses diet-induced hepatic steatosis and insulin resistance. J Biol Chem 2007 282 22678-22688
15. Yu XX, Murray SF, Watts L, Booten SL, Tokorcheck J, Monia BP, Bhanot S. Reduction of JNK1 expression with antisense oligonucleotide improves adiposity in obese mice. Am J Physiol Endocrinol Metab 2008 295 E436-E445
16. Yu XX, Pandey SK, Booten SL, Murray SF, Monia BP, Bhanot S. Reduced adiposity and improved insulin sensitivity in obese mice with antisense suppression of 4E-BP2 expression. Am J Physiol Endocrinol Metab 2008 294 E530-E539
17. Dean NM, Butler M, Monia BP, Manoharan M. Pharmacology of 2-O-(2-methoxy)ethyl-modified antisense oligonucleotide. In: Crooke ST (ed). Antisense Drug Technology: Principles, Strategies and Applications New York: Dekker 2001 319-338
18. Pandey SK, Yu XX, Watts LM, Michael MD, Sloop KW, Rivard AR, Leedom TA, Manchem VP, Samadzadeh L, McKay RA, Monia BP, Bhanot S. Reduction of low molecular weight protein-tyrosine phosphatase expression improves hyperglycemia and insulin sensitivity in obese mice. J Biol Chem 2007 282 14291-14299
19. Rahman F, Heller T, Sobao Y, Mizukoshi E, Nascimbeni M, Alter H, Herrine S, Hoofnagle J, Liang TJ, Rehermann B. Effects of antiviral therapy on the cellular immune response in acute hepatitis C. Hepatology 2004 40 87-97
20. Barbour LA, Mizanoor RS, Gurevich I, Leitner JW, Fischer SJ, Roper MD, Knotts TA, Vo Y, McCurdy CE, Yakar S, Leroith D, Kahn CR, Cantley LC, Friedman JE, Draznin B. Increased P85alpha is a potent negative regulator of skeletal muscle insulin signaling and induces in vivo insulin resistance associated with growth hormone excess. J Biol Chem 2005 280 37489-37494
21. Draznin B. Molecular mechanisms of insulin resistance: serine phosphorylation of insulin receptor substrate-1 and increased expression of p85alpha: the two sides of a coin. Diabetes 2006 55 2392-2397
22. Li P, Koike T, Qin B, Kubota M, Kawata Y, Jia YJ, Oshida Y. A high-fructose diet impairs Akt and PKC phophorylation and GLUT4 translocation in rat skeletal muscle. Horm Met Res 2008 40 528-532