Engineering a ubiquitin ligase reveals conformational flexibility required for ubiquitin transfer

Journal of Biological Chemistry

Volumn 284, Issue 39, 2009, Pages 26797-26802

  Qian, Shu Bing ^a,b   Waldron, Lauren ^b   Choudhary, Neelima ^b   Klevit, Rachel E ^c   Chazin, Walter J ^d   Patterson, Cam ^b  

^a Department of Obstetrics Gynecology   (United States)

^b UNIVERSITY OF NORTH CAROLINA SCHOOL OF MEDICINE   (United States)

^c UNIVERSITY OF WASHINGTON SCHOOL OF MEDICINE   (United States)

^d VANDERBILT UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CARBOXYL TERMINUS; CELLULAR FUNCTION; COMPUTATIONAL STRUCTURE; CONFORMATIONAL CHANGE; CONFORMATIONAL FLEXIBILITY; DIRECT CONTACT; PRECISE POSITIONING; PROTEIN ENGINEERING; RESEARCH TOOLS; SUBSTRATE SPECIFICITY; TARGET-SUBSTRATE; UBIQUITIN; UBIQUITIN LIGASES; UBIQUITIN-CONJUGATING ENZYMES; UBIQUITINATION;

BIOCHEMICAL ENGINEERING; ENZYMES; GENETIC ENGINEERING;

SUBSTRATES;

HEAT SHOCK PROTEIN 70; UBIQUITIN; UBIQUITIN PROTEIN LIGASE; UBIQUITIN PROTEIN LIGASE E2; UBIQUITIN PROTEIN LIGASE E3; UNCLASSIFIED DRUG;

ARTICLE; BIOENGINEERING; CARBOXY TERMINAL SEQUENCE; ENZYME CONFORMATION; ENZYME SPECIFICITY; ENZYME SUBSTRATE; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN DEGRADATION; PROTEIN PROTEIN INTERACTION; PROTEIN STRUCTURE; PROTEIN TARGETING; PROTEIN TRANSPORT; UBIQUITINATION;

BINDING SITES; CATALYSIS; CRYSTALLIZATION; CRYSTALLOGRAPHY, X-RAY; HUMANS; MODELS, MOLECULAR; MUTATION; PROTEIN CONFORMATION; PROTEIN ENGINEERING; PROTEIN MULTIMERIZATION; PROTEIN STRUCTURE, TERTIARY; SUBSTRATE SPECIFICITY; UBIQUITIN; UBIQUITIN-CONJUGATING ENZYMES; UBIQUITIN-PROTEIN LIGASES; UBIQUITINATION;

EUKARYOTA;

EID: 70350365502     PISSN: 00219258     EISSN: 1083351X     Source Type: Journal    
DOI: 10.1074/jbc.M109.032334     Document Type: Article

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