Structure of the Cul1-Rbx1-Skp1-F boxSkp2 SCF ubiquitin ligase complex

Nature

Volumn 416, Issue 6882, 2002, Pages 703-709

  Zheng, Ning ^a,b   Schulman, Brenda A ^a   Song, Langzhou ^a,b   Miller, Julie J ^a,b   Jeffrey, Philip D ^a   Wang, Ping ^a   Chu, Claire ^b,c   Koepp, Deanna M ^c   Elledge, Stephen J ^b,c   Pagano, Michele ^d   Conaway, Ronald C ^e   Conaway, Joan W ^e   Harper, J Wade ^c   Pavletich, Nikola P ^a,b  

^a Cell Biochem and Biophys Program   (United States)

^b MEMORIAL SLOAN KETTERING CANCER CENTER   (United States)

^c BAYLOR COLLEGE OF MEDICINE   (United States)

^d NEW YORK UNIVERSITY MEDICAL CENTER   (United States)

^e STOWERS INSTITUTE FOR MEDICAL RESEARCH   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CATALYSIS; CRYSTAL STRUCTURE; ENZYMES; MUTAGENESIS; SUBSTRATES;

UBIQUITINATION;

BIOCHEMISTRY;

CULLIN; F BOX PROTEIN; PROTEIN P27; RBX1 PROTEIN; S PHASE KINASE ASSOCIATED PROTEIN 2; SKP1 PROTEIN; UBIQUITIN CONJUGATING ENZYME; UBIQUITIN PROTEIN LIGASE; UNCLASSIFIED DRUG;

PROTEIN;

ARTICLE; BINDING SITE; COMPLEX FORMATION; CRYSTAL STRUCTURE; CRYSTALLIZATION; ENZYME STRUCTURE; HUMAN; HYDROGEN BOND; MOLECULAR INTERACTION; NONHUMAN; PRIORITY JOURNAL; STRUCTURE ANALYSIS;

AMINO ACID MOTIFS; AMINO ACID SEQUENCE; BINDING SITES; CELL CYCLE PROTEINS; CRYSTALLOGRAPHY, X-RAY; CULLIN PROTEINS; CYCLIN-DEPENDENT KINASE INHIBITOR P27; HUMANS; LIGASES; MACROMOLECULAR SUBSTANCES; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; PEPTIDE SYNTHASES; PROTEIN BINDING; PROTEIN STRUCTURE, QUATERNARY; PROTEIN STRUCTURE, TERTIARY; S-PHASE KINASE-ASSOCIATED PROTEINS; SEQUENCE ALIGNMENT; SEQUENCE HOMOLOGY, AMINO ACID; SKP CULLIN F-BOX PROTEIN LIGASES; STRUCTURE-ACTIVITY RELATIONSHIP; TUMOR SUPPRESSOR PROTEINS; UBIQUITIN; UBIQUITIN-CONJUGATING ENZYMES; UBIQUITIN-PROTEIN LIGASES; UBIQUITINS;

EID: 18344391432     PISSN: 00280836     EISSN: None     Source Type: Journal    
DOI: 10.1038/416703a     Document Type: Article

References (50)

1. The ubiquitin system
2. SCF and Cullin/Ring H2-based ubiquitin ligases
3. How the cyclin became a cyclin: Regulated proteolysis in the cell cycle
4. Human F-box protein hCdc4 targets cyclin E for proteolysis and is mutated in a breast cancer cell line
5. Phosphorylation-dependent ubiquitination of cyclin E by the SCFFbw 7 ubiquitin ligase
6. Archipelago regulates Cyclin E levels in Drosophila and is mutated in human cancer cell lines
7. Wnt signaling and cancer
8. Regulation of the edk inhibitor p27 and its deregulation in cancer
9. Role of the F-box protein Skp2 in lymphomagenesis
10. Skp2 is oncogenic and overexpressed in human cancers
11. Identification of the von Hippel-Lindau disease tumor suppressor gene
12. Structure of the VHL-EIonginC-EIonginB complex: Implications for VHL tumor suppressor function
13. Mechanisms underlying ubiquitination
14. RING finger proteins: Mediators of ubiquitin ligase activity
15. SKP1 connects cell cycle regulators to the ubiquitin proteolysis machinery through a novel motif, the F-box
16. F-box proteins are receptors that recruit phosphorylated substrates to the SCF ubiquitin-ligase complex
17. A complex of Cdc4p, Skplp, and Cdc53p/cullin catalyzes ubiquitination of the phosphorylated CDK inhibitor Siclp
18. Reconstitution of G1 cyclin ubiquitination with complexes containing SCFGrr1 and Rbx1
19. Rbx1, a component of the VHL tumor suppressor complex and SCF ubiquitin ligase
20. ROCI, a homolog of APC11, represents a family of cullin partners with an associated ubiquitin ligase activity
21. Cdc53/cullin and the essential Hrt1 RING-H2 subunit of SCF define a ubiquitin ligase module that activates the E2 enzyme Cdc34
22. The F-box protein family
23. The cell-cycle regulatory protein Cksl is required for SCF (Skp2)-mediated ubiquitinylation of p27
24. A CDK-independent function of mammalian Cksl. Targeting of SCF (Skp2) to the CDK inhibitor p27 (Kipl)
25. cul-1 is required for cell cycle exit in C. elegans and identifies a novel gene family
26. The von Hippel-Lindau tumor suppressor protein
27. Mass spectrometric analysis of the anaphase-promoting complex from yeast: Identification of a subunit related to cullins
28. Identification of a cullin homology region in a subunit of the anaphase-promoting complex
29. Insights into SCF ubiquitin ligases from the structure of the Skp1-Skp2 complex
30. Crystal structure of the vesicular transport protein Sec17: Implications for SNAP function in SNARE complex disassembly
31. Cdc53p acts in concert with Cdc4p and Cdc34p to control the G1-to-S-phase transition and identifies a conserved family of proteins
32. Characterization of the DOC1/APC10 subunit of the yeast and the human anaphase-promoting complex
33. Structure of a c-Cbl-UbcH7 complex: RING domain function in ubiquitin-protein ligases
34. The conserved RING-H2 finger of ROC1 is required for ubiquitin ligation
35. Nedd8 modification of cul-1 activates SCF (beta(TrCP))-dependent ubiquitination of IkappaBalpha
36. Modification of cullin-1 by ubiquitin-like protein Nedd8 enhances the activity ofSCF (skp2) toward p27 (kip 1)
37. NEDD8 recruits E2-ubiquitin to SCF E3 ligase
38. The Nedd8-conjugated ROCI-CUL1 core ubiquitin ligase utilizes Nedd8 charged surface residues for efficient polyubiquitin chain assembly catalyzed by Cdc34
39. Down-regulation of p27 (Kip1) by two mechanisms, ubiquitin-mediated degradation and proteolytic processing
40. Cdc53 is a scaffold protein for multiple Cdc34/Skp1/F-box protein complexes that regulate cell division and methionine biosynthesis in yeast
41. Signal-induced degradation of l kappa B alpha requires site-specific ubiquitination
42. Smad3 recruits the anaphase-promoting complex for ubiquitination and degradation of SnoN
43. Processing of x-ray diffraction data collected in oscillation mode
44. Maximum-likelihood heavy-atom parameter refinement for the multiple isomorphous replacement and multiwavelength anomalous diffraction methods
45. Crystallography and NMR system: A new software suite for macromolecular structure determination
46. Improved methods for binding protein models in electron density maps and the location of errors in these models
47. CCP4 The CCP4 suite: Programs for protein crystallography
48. Crystal structure of the p27Kip1 cyclin-dependent-kinase inhibitor bound to the cyclin A-Cdk2 complex
49. MOLSCRIPT: A program to produce both detailed and schematic plots of protein structures
50. Protein folding and association: Insight from the interfacial and thermodynamic properties of hydrocarbons