Novel genes retrieved from environmental DNA by polymerase chain reaction: Current genome-walking techniques for future metagenome applications

Journal of Biotechnology

Volumn 144, Issue 2, 2009, Pages 75-82

  Kotik, Michael ^a  

^a INSTITUTE OF MICROBIOLOGY   (Czech Republic)

Author keywords

Consensus sequence; DNA cassette; Genome walking; Metagenome; PCR

Indexed keywords

ACTIVITY-BASED; BIOCATALYTIC ACTIVITY; CONSENSUS PRIMERS; CONSENSUS SEQUENCE; GENE MINING; GENES ENCODING; GENES ENCODING ENZYMES; GENOME WALKING; GENOMIC DNA; METAGENOME; METAGENOMES; PCR; POLYMERASE CHAIN REACTION; POTENTIAL APPLICATIONS; RECOMBINANT STRAINS;

CLONING; ENCODING (SYMBOLS); ENZYMES; GENE ENCODING; MINING; NUCLEIC ACIDS;

DNA;

DNA FRAGMENT; GENOMIC DNA; RESTRICTION ENDONUCLEASE; SINGLE STRANDED DNA;

BIOCATALYST; BIOTECHNOLOGY; CONSENSUS SEQUENCE; ENZYME ACTIVITY; GENE AMPLIFICATION; GENE CASSETTE; GENE MAPPING; GENETIC SCREENING; GENOME WALKING; METAGENOMICS; NUCLEOTIDE SEQUENCE; PHOSPHORYLATION; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; REVIEW; SENSITIVITY AND SPECIFICITY; SEQUENCE ALIGNMENT; SEQUENCE ANALYSIS;

CHROMOSOME WALKING; DNA; ENVIRONMENT; GENES; METAGENOME; METAGENOMICS; POLYMERASE CHAIN REACTION;

EID: 70350236508     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2009.08.013     Document Type: Review

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